Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)

Drought is among the most important natural disasters with severe effects on animals and plants. MicroRNAs are a class of noncoding RNAs that play a crucial role in plant growth, development, and response to stress factors, including drought. However, the microRNAs in drought responses in common vet...

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Auteurs principaux:	Zhu Yongqun, Liu Qiuxu, Xu Wenzhi, Yao Li, Wang Xie, Wang Hong, Xu Yalin, Li Linxiang, Duan Chunhua, Yi Zhixin, Lin Chaowen
Format:	article
Langue:	EN
Publié:	De Gruyter 2021
Sujets:	common vetch vicia sativa microrna drought stress high-throughput sequencing target gene Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/883826184c2447fe83eeba10a56ddcdb
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spelling	oai:doaj.org-article:883826184c2447fe83eeba10a56ddcdb2021-12-05T14:10:42ZIdentification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)2391-541210.1515/biol-2021-0109https://doaj.org/article/883826184c2447fe83eeba10a56ddcdb2021-10-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0109https://doaj.org/toc/2391-5412Drought is among the most important natural disasters with severe effects on animals and plants. MicroRNAs are a class of noncoding RNAs that play a crucial role in plant growth, development, and response to stress factors, including drought. However, the microRNAs in drought responses in common vetch (Vicia sativa), an annual herbaceous leguminous plant commonly used for forage by including it in mixed seeding during winter and spring, have not been characterized. To explore the microRNAs’ response to drought in common vetch, we sequenced 10 small RNA (sRNA) libraries by the next-generation sequencing technology. We obtained 379 known miRNAs belonging to 38 families and 47 novel miRNAs. The two groups had varying numbers of differentially expressed miRNAs: 85 in the comparison group D5 vs C5 and 38 in the comparison group D3 vs C3. Combined analysis of mRNA and miRNA in the same samples under drought treatment identified 318 different target genes of 123 miRNAs. Functional annotation of the target genes revealed that the miRNAs regulate drought-responsive genes, such as leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs), ABC transporter G family member 1 (ABCG1), and MAG2-interacting protein 2 (MIP2). The genes were involved in various pathways, including cell wall biosynthesis, reactive oxygen removal, and protein transport. The findings in this study provide new insights into the miRNA-mediated regulatory networks of drought stress response in common vetch.Zhu YongqunLiu QiuxuXu WenzhiYao LiWang XieWang HongXu YalinLi LinxiangDuan ChunhuaYi ZhixinLin ChaowenDe Gruyterarticlecommon vetchvicia sativamicrornadrought stresshigh-throughput sequencingtarget geneBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1111-1121 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	common vetch vicia sativa microrna drought stress high-throughput sequencing target gene Biology (General) QH301-705.5
spellingShingle	common vetch vicia sativa microrna drought stress high-throughput sequencing target gene Biology (General) QH301-705.5 Zhu Yongqun Liu Qiuxu Xu Wenzhi Yao Li Wang Xie Wang Hong Xu Yalin Li Linxiang Duan Chunhua Yi Zhixin Lin Chaowen Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
description	Drought is among the most important natural disasters with severe effects on animals and plants. MicroRNAs are a class of noncoding RNAs that play a crucial role in plant growth, development, and response to stress factors, including drought. However, the microRNAs in drought responses in common vetch (Vicia sativa), an annual herbaceous leguminous plant commonly used for forage by including it in mixed seeding during winter and spring, have not been characterized. To explore the microRNAs’ response to drought in common vetch, we sequenced 10 small RNA (sRNA) libraries by the next-generation sequencing technology. We obtained 379 known miRNAs belonging to 38 families and 47 novel miRNAs. The two groups had varying numbers of differentially expressed miRNAs: 85 in the comparison group D5 vs C5 and 38 in the comparison group D3 vs C3. Combined analysis of mRNA and miRNA in the same samples under drought treatment identified 318 different target genes of 123 miRNAs. Functional annotation of the target genes revealed that the miRNAs regulate drought-responsive genes, such as leucine-rich repeat receptor-like kinase-encoding genes (LRR-RLKs), ABC transporter G family member 1 (ABCG1), and MAG2-interacting protein 2 (MIP2). The genes were involved in various pathways, including cell wall biosynthesis, reactive oxygen removal, and protein transport. The findings in this study provide new insights into the miRNA-mediated regulatory networks of drought stress response in common vetch.
format	article
author	Zhu Yongqun Liu Qiuxu Xu Wenzhi Yao Li Wang Xie Wang Hong Xu Yalin Li Linxiang Duan Chunhua Yi Zhixin Lin Chaowen
author_facet	Zhu Yongqun Liu Qiuxu Xu Wenzhi Yao Li Wang Xie Wang Hong Xu Yalin Li Linxiang Duan Chunhua Yi Zhixin Lin Chaowen
author_sort	Zhu Yongqun
title	Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
title_short	Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
title_full	Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
title_fullStr	Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
title_full_unstemmed	Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)
title_sort	identification of novel drought-responsive mirna regulatory network of drought stress response in common vetch (vicia sativa)
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/883826184c2447fe83eeba10a56ddcdb
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Identification of novel drought-responsive miRNA regulatory network of drought stress response in common vetch (Vicia sativa)

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