Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production

Abstract Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate–po...

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Autores principales: Asmaa Abdella, Samah Ramadan, Ragaa A. Hamouda, Amna A. Saddiq, Nuha M. Alhazmi, Mahmoud A. Al-Saman
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:8838e157c8704bc397a86679f29ad6372021-12-02T18:51:00ZPaecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production10.1038/s41598-021-95965-w2045-2322https://doaj.org/article/8838e157c8704bc397a86679f29ad6372021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95965-whttps://doaj.org/toc/2045-2322Abstract Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirming its monomeric nature as single band at 32 KDa. Zymography showed xylan hydrolysis activity at the same band. The purified enzyme had optimum activity at 60 °C and pH 5.0. The pH stability range was 5–9 and the temperature stability was up 70 °C. Fe2+and Fe3+ exhibited inhibition of xylanase enzyme while Cu2+, Ca2+, Mg2+ and Mn2+ stimulated its activity. Mercaptoethanol stimulated its activity; however, Na2-EDTA and SDS inhibited its activity. The purified xylanase could hydrolyze beechwood xylan but not carboxymethyl cellulose (CMC), avicel or soluble starch. Paecilomyces variotii xylanase Km and Vmax for beechwood were determined to be 3.33 mg/mL and 5555 U/mg, respectively. The produced xylanase enzyme applied on beech xylan resulted in different types of XOS. The antioxidant activity of xylo-oligosaccharides increased from 15.22 to 70.57% when the extract concentration was increased from 0.1 to 1.5 mg/mL. The enzyme characteristics and kinetic parameters indicated its high efficiency in the hydrolysis of xylan and its potential effectiveness in lignocellulosic hydrolysis and other industrial application. It also suggests the potential of xylanase enzyme for production of XOS from biomass which are useful in food and pharmaceutical industries.Asmaa AbdellaSamah RamadanRagaa A. HamoudaAmna A. SaddiqNuha M. AlhazmiMahmoud A. Al-SamanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Asmaa Abdella
Samah Ramadan
Ragaa A. Hamouda
Amna A. Saddiq
Nuha M. Alhazmi
Mahmoud A. Al-Saman
Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
description Abstract Paecilomyces variotii xylanase was, produced in stirred tank bioreactor with yield of 760 U/mL and purified using 70% ammonium sulfate precipitation and ultra-filtration causing 3.29-fold purification with 34.47% activity recovery. The enzyme purity was analyzed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirming its monomeric nature as single band at 32 KDa. Zymography showed xylan hydrolysis activity at the same band. The purified enzyme had optimum activity at 60 °C and pH 5.0. The pH stability range was 5–9 and the temperature stability was up 70 °C. Fe2+and Fe3+ exhibited inhibition of xylanase enzyme while Cu2+, Ca2+, Mg2+ and Mn2+ stimulated its activity. Mercaptoethanol stimulated its activity; however, Na2-EDTA and SDS inhibited its activity. The purified xylanase could hydrolyze beechwood xylan but not carboxymethyl cellulose (CMC), avicel or soluble starch. Paecilomyces variotii xylanase Km and Vmax for beechwood were determined to be 3.33 mg/mL and 5555 U/mg, respectively. The produced xylanase enzyme applied on beech xylan resulted in different types of XOS. The antioxidant activity of xylo-oligosaccharides increased from 15.22 to 70.57% when the extract concentration was increased from 0.1 to 1.5 mg/mL. The enzyme characteristics and kinetic parameters indicated its high efficiency in the hydrolysis of xylan and its potential effectiveness in lignocellulosic hydrolysis and other industrial application. It also suggests the potential of xylanase enzyme for production of XOS from biomass which are useful in food and pharmaceutical industries.
format article
author Asmaa Abdella
Samah Ramadan
Ragaa A. Hamouda
Amna A. Saddiq
Nuha M. Alhazmi
Mahmoud A. Al-Saman
author_facet Asmaa Abdella
Samah Ramadan
Ragaa A. Hamouda
Amna A. Saddiq
Nuha M. Alhazmi
Mahmoud A. Al-Saman
author_sort Asmaa Abdella
title Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
title_short Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
title_full Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
title_fullStr Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
title_full_unstemmed Paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
title_sort paecilomyces variotii xylanase production, purification and characterization with antioxidant xylo-oligosaccharides production
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/8838e157c8704bc397a86679f29ad637
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AT ragaaahamouda paecilomycesvariotiixylanaseproductionpurificationandcharacterizationwithantioxidantxylooligosaccharidesproduction
AT amnaasaddiq paecilomycesvariotiixylanaseproductionpurificationandcharacterizationwithantioxidantxylooligosaccharidesproduction
AT nuhamalhazmi paecilomycesvariotiixylanaseproductionpurificationandcharacterizationwithantioxidantxylooligosaccharidesproduction
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