Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring

Background: The treatment of symptoms of prostatic hyperplasia is among the traditional uses of Achyrocline bogotensis (Kunth) [N.V. “Vira Vira”, Compositae] in Colombia. Pharmacological therapy for this disorder depends mainly on alpha-1 antiadrenergic agents, and the mechanism has not been studie...

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Autores principales:	Nidya LARA, Javier RINCÓN, Mario F. GUERRERO
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2017
Materias:	Achyroclines antiadrenergics male lower urinary tract symptoms (LUTS) phenylephrine aorta flavonoids Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/883d8021b91144b281079c73825d4780
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spelling	oai:doaj.org-article:883d8021b91144b281079c73825d47802021-11-19T04:09:08ZAlpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring0121-40042145-2660https://doaj.org/article/883d8021b91144b281079c73825d47802017-04-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/325075https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: The treatment of symptoms of prostatic hyperplasia is among the traditional uses of Achyrocline bogotensis (Kunth) [N.V. “Vira Vira”, Compositae] in Colombia. Pharmacological therapy for this disorder depends mainly on alpha-1 antiadrenergic agents, and the mechanism has not been studied previously using A. bogotensis. Objectives: To assess the alpha-1 antiadrenergic effect of the extract obtained from the aerial parts of A. bogotensis in isolated aortic rings from Wistar rats. Methods: The study compared the effects of the ethanol extract of A. bogotensis, prazosin (reference) and DMSO (control) in rings stimulated with phenylephrine (PE) or KCl. The capacity to reduce the PE pressor effect by the ethanol extract (pD2’ value) was determined. To quantify the A. bogotensis relaxant potency, increasing concentrations of the ethanol extract (0.1 µg/mL-0.1 mg/mL), were added cumulatively to isolated aortic rings pre-contracted with PE (0.1 µM) or KCl (80 mM). To explore the possible participation of nitric oxide (NO), L-NAME (100 µM) was administered to aortic rings exposed to cumulatively increasing concentrations of PE in isolated aortic rings in the presence of the extract (10 µg/mL). Aqueous, butanol and dichloromethane fractions (10 µg/mL) obtained from the ethanol extract were assayed. Phytochemical screening was also performed. Results: Prazosin and A. bogotensis extract notably reduced the contraction induced by PE whereas their inhibitory effect in rings contracted with KCl were lower. A. bogotensis ethanol extract showed a high capacity for reducing the PE pressor response (pD´2 : 5.51) as well as total efficacy for relaxing rings previously precontracted with PE. The relaxant efficacy and potency of A. bogotensis extract against rings previously contracted with KCl were notably lower. L-NAME partly reverted the inhibitory effect of A. bogotensis. Aqueous, butanol and dichloromethane fractions gave inhibitory responses lower than that obtained with the ethanol extract. Phytochemical screening of A. bogotensis extract revealed the significant presence of flavonoid and triterpene metabolites. Conclusions: These results suggest that A. bogotensis elicits a smooth muscle relaxant effect related to the alpha-1 antiadrenergic mechanism. This response is partially NO dependent and seems to be due to interactions among active metabolites likely to be of flavonoid and/or terpenoid nature. Nidya LARAJavier RINCÓNMario F. GUERREROUniversidad de AntioquiaarticleAchyroclinesantiadrenergicsmale lower urinary tract symptoms (LUTS)phenylephrineaortaflavonoidsFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 24, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Achyroclines antiadrenergics male lower urinary tract symptoms (LUTS) phenylephrine aorta flavonoids Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Achyroclines antiadrenergics male lower urinary tract symptoms (LUTS) phenylephrine aorta flavonoids Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Nidya LARA Javier RINCÓN Mario F. GUERRERO Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
description	Background: The treatment of symptoms of prostatic hyperplasia is among the traditional uses of Achyrocline bogotensis (Kunth) [N.V. “Vira Vira”, Compositae] in Colombia. Pharmacological therapy for this disorder depends mainly on alpha-1 antiadrenergic agents, and the mechanism has not been studied previously using A. bogotensis. Objectives: To assess the alpha-1 antiadrenergic effect of the extract obtained from the aerial parts of A. bogotensis in isolated aortic rings from Wistar rats. Methods: The study compared the effects of the ethanol extract of A. bogotensis, prazosin (reference) and DMSO (control) in rings stimulated with phenylephrine (PE) or KCl. The capacity to reduce the PE pressor effect by the ethanol extract (pD2’ value) was determined. To quantify the A. bogotensis relaxant potency, increasing concentrations of the ethanol extract (0.1 µg/mL-0.1 mg/mL), were added cumulatively to isolated aortic rings pre-contracted with PE (0.1 µM) or KCl (80 mM). To explore the possible participation of nitric oxide (NO), L-NAME (100 µM) was administered to aortic rings exposed to cumulatively increasing concentrations of PE in isolated aortic rings in the presence of the extract (10 µg/mL). Aqueous, butanol and dichloromethane fractions (10 µg/mL) obtained from the ethanol extract were assayed. Phytochemical screening was also performed. Results: Prazosin and A. bogotensis extract notably reduced the contraction induced by PE whereas their inhibitory effect in rings contracted with KCl were lower. A. bogotensis ethanol extract showed a high capacity for reducing the PE pressor response (pD´2 : 5.51) as well as total efficacy for relaxing rings previously precontracted with PE. The relaxant efficacy and potency of A. bogotensis extract against rings previously contracted with KCl were notably lower. L-NAME partly reverted the inhibitory effect of A. bogotensis. Aqueous, butanol and dichloromethane fractions gave inhibitory responses lower than that obtained with the ethanol extract. Phytochemical screening of A. bogotensis extract revealed the significant presence of flavonoid and triterpene metabolites. Conclusions: These results suggest that A. bogotensis elicits a smooth muscle relaxant effect related to the alpha-1 antiadrenergic mechanism. This response is partially NO dependent and seems to be due to interactions among active metabolites likely to be of flavonoid and/or terpenoid nature.
format	article
author	Nidya LARA Javier RINCÓN Mario F. GUERRERO
author_facet	Nidya LARA Javier RINCÓN Mario F. GUERRERO
author_sort	Nidya LARA
title	Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
title_short	Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
title_full	Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
title_fullStr	Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
title_full_unstemmed	Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring
title_sort	alpha antiadrenergic effect of achyrocline bogotensis extract (“vira vira”) in isolated rat aortic ring
publisher	Universidad de Antioquia
publishDate	2017
url	https://doaj.org/article/883d8021b91144b281079c73825d4780
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Alpha antiadrenergic effect of Achyrocline bogotensis extract (“Vira Vira”) in isolated rat aortic ring

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