Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models

Scientists often model complex biological phenomena in vitro, mimicking conditions found in living organisms. Understanding the power and limitations of biological models is an important topic in undergraduate science. In this activity, students develop their own in vitro model for testing the survi...

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Autor principal:	Srebrenka Robic
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/884476bfa8d34d859465da2fe1cdc208
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spelling	oai:doaj.org-article:884476bfa8d34d859465da2fe1cdc2082021-11-15T15:04:04ZLaboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models10.1128/jmbe.v11.i1.1391935-78851935-7877https://doaj.org/article/884476bfa8d34d859465da2fe1cdc2082010-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v11.i1.139https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Scientists often model complex biological phenomena in vitro, mimicking conditions found in living organisms. Understanding the power and limitations of biological models is an important topic in undergraduate science. In this activity, students develop their own in vitro model for testing the survival of bacteria from commercial probiotic supplements. Students work in groups to decide which factors are important for survival of bacteria in a chosen portion of the human digestive tract. Groups of students create their own in vitro models of organs such as stomach and/or intestines. Students expose a probiotic supplement to conditions mimicking the chosen portion of the human digestive tract, and measure the effect of those conditions on the survival of bacteria found in the supplement. Students choose to focus on conditions such as low pH found in stomach or pancreatic enzymes found in the upper intestine. Through this activity, students gain experience with serial dilutions and calculations of colony forming units (CFUs). This project also provides the students with the valuable experience of designing experiments in small groups. Students present their findings in a poster session, which provides a venue for discussing the validity and limitation of various models.Srebrenka RobicAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 11, Iss 1, Pp 50-55 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Srebrenka Robic Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
description	Scientists often model complex biological phenomena in vitro, mimicking conditions found in living organisms. Understanding the power and limitations of biological models is an important topic in undergraduate science. In this activity, students develop their own in vitro model for testing the survival of bacteria from commercial probiotic supplements. Students work in groups to decide which factors are important for survival of bacteria in a chosen portion of the human digestive tract. Groups of students create their own in vitro models of organs such as stomach and/or intestines. Students expose a probiotic supplement to conditions mimicking the chosen portion of the human digestive tract, and measure the effect of those conditions on the survival of bacteria found in the supplement. Students choose to focus on conditions such as low pH found in stomach or pancreatic enzymes found in the upper intestine. Through this activity, students gain experience with serial dilutions and calculations of colony forming units (CFUs). This project also provides the students with the valuable experience of designing experiments in small groups. Students present their findings in a poster session, which provides a venue for discussing the validity and limitation of various models.
format	article
author	Srebrenka Robic
author_facet	Srebrenka Robic
author_sort	Srebrenka Robic
title	Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
title_short	Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
title_full	Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
title_fullStr	Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
title_full_unstemmed	Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models
title_sort	laboratory exploration of survival of probiotic cultures inside the human digestive tract using <italic toggle="yes">in vitro</italic> models
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/884476bfa8d34d859465da2fe1cdc208
work_keys_str_mv	AT srebrenkarobic laboratoryexplorationofsurvivalofprobioticculturesinsidethehumandigestivetractusingitalictoggleyesinvitroitalicmodels
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Laboratory Exploration of Survival of Probiotic Cultures Inside the Human Digestive Tract Using <italic toggle="yes">In Vitro</italic> Models

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