Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.

Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cel...

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Autores principales:	Soumya Roy Chowdhury, Handanahal S Savithri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88480e7feb34496e877e4761a06f9ba4
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spelling	oai:doaj.org-article:88480e7feb34496e877e4761a06f9ba42021-11-18T07:00:46ZInteraction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.1932-620310.1371/journal.pone.0015609https://doaj.org/article/88480e7feb34496e877e4761a06f9ba42011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21246040/?tool=EBIhttps://doaj.org/toc/1932-6203Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cell movement of the virus, all the proteins encoded by SeMV genome were cloned into yeast Matchmaker system 3 and interaction studies were performed. Two proteins namely, viral protein genome linked (VPg) and a 10-kDa protein (P10) c v gft encoded by OFR 2a, were identified as possible interacting partners in addition to the viral coat protein (CP). Further characterization of these interactions revealed that the movement protein (MP) recognizes cognate RNA through interaction with VPg, which is covalently linked to the 5' end of the RNA. Analysis of the deletion mutants delineated the domains of MP involved in the interaction with VPg and P10. This study implicates for the first time that VPg might play an important role in specific recognition of viral genome by MP in SeMV and shed light on the possible role of P10 in the viral movement.Soumya Roy ChowdhuryHandanahal S SavithriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15609 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soumya Roy Chowdhury Handanahal S Savithri Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
description	Sesbania mosaic virus (SeMV) is a single strand positive-sense RNA plant virus that belongs to the genus Sobemovirus. The mechanism of cell-to-cell movement in sobemoviruses has not been well studied. With a view to identify the viral encoded ancillary proteins of SeMV that may assist in cell-to-cell movement of the virus, all the proteins encoded by SeMV genome were cloned into yeast Matchmaker system 3 and interaction studies were performed. Two proteins namely, viral protein genome linked (VPg) and a 10-kDa protein (P10) c v gft encoded by OFR 2a, were identified as possible interacting partners in addition to the viral coat protein (CP). Further characterization of these interactions revealed that the movement protein (MP) recognizes cognate RNA through interaction with VPg, which is covalently linked to the 5' end of the RNA. Analysis of the deletion mutants delineated the domains of MP involved in the interaction with VPg and P10. This study implicates for the first time that VPg might play an important role in specific recognition of viral genome by MP in SeMV and shed light on the possible role of P10 in the viral movement.
format	article
author	Soumya Roy Chowdhury Handanahal S Savithri
author_facet	Soumya Roy Chowdhury Handanahal S Savithri
author_sort	Soumya Roy Chowdhury
title	Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
title_short	Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
title_full	Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
title_fullStr	Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
title_full_unstemmed	Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.
title_sort	interaction of sesbania mosaic virus movement protein with vpg and p10: implication to specificity of genome recognition.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/88480e7feb34496e877e4761a06f9ba4
work_keys_str_mv	AT soumyaroychowdhury interactionofsesbaniamosaicvirusmovementproteinwithvpgandp10implicationtospecificityofgenomerecognition AT handanahalssavithri interactionofsesbaniamosaicvirusmovementproteinwithvpgandp10implicationtospecificityofgenomerecognition
_version_	1718424054563602432

Interaction of Sesbania mosaic virus movement protein with VPg and P10: implication to specificity of genome recognition.

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