Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia

Binur R, Pancoro A. 2017. Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia. Biodiversitas 18: 609-618. Inbreeding accumulation will tend to reduce genetic variation or depressed of the prawn fry produced. This problem...

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Autores principales:	ROBI BINUR, ADI PANCORO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	freshwater prawn macrobrachium rosenbergii post-larvae microsatellite inbreeding depression heterozygosity genetic diversity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/88518682ae4f4bd59ddb6b1b9504b498
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spelling	oai:doaj.org-article:88518682ae4f4bd59ddb6b1b9504b4982021-11-16T13:52:31ZInbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia1412-033X2085-472210.13057/biodiv/d180223https://doaj.org/article/88518682ae4f4bd59ddb6b1b9504b4982017-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/1678https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Binur R, Pancoro A. 2017. Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia. Biodiversitas 18: 609-618. Inbreeding accumulation will tend to reduce genetic variation or depressed of the prawn fry produced. This problem has caused a decrease in production and quality of prawns culture in Indonesia. The purpose of this study is to measure the level of inbreeding depression prawn fry generated from several hatcheries in Java by microsatellite markers. There is four microsatellite locus to be used i.e Prk9A/T1, Prk4G/T1, TGFP16, and Mr8-88. The amplification of fourth locus using PCR with 6-carboxy-fluorescine (6-FAM) label. The number of alleles (Na) from fourth locus is Mr8-88 (11 alleles), TGFP16 (10 alleles), Prk4G/T1 (9 alleles), dan Prk9A/T1 (5 alleles), respectively. The level of polymorphism locus from highest to lowest is locus Prk4G/T1 (0.703), Prk9A/T1 (0.507), TGFP16 (0.410), and Mr8-88 (0.370), respectively. Inbreeding depression level of postlarvae (PL) M. rosenbergii tend to moderate with BBI Ciamis (He 0.444), BBUG Samas (He 0.514), LRPTBPAT Sukamandi (He 0.519), and UPBL Probolinggo (He 0.530), respectively. AMOVA analysis showed about 8.0% genetic variation among populations. From these results, it can be concluded that the PL produced indicated have been depressed. Post-larvae prawns produced from fourth hatcheries is not recommended to be a broodstock but can be used for cultivation for farmers.ROBI BINURADI PANCOROMBI & UNS Soloarticlefreshwater prawnmacrobrachium rosenbergiipost-larvaemicrosatelliteinbreeding depressionheterozygositygenetic diversityBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 2, Pp 609-618 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	freshwater prawn macrobrachium rosenbergii post-larvae microsatellite inbreeding depression heterozygosity genetic diversity Biology (General) QH301-705.5
spellingShingle	freshwater prawn macrobrachium rosenbergii post-larvae microsatellite inbreeding depression heterozygosity genetic diversity Biology (General) QH301-705.5 ROBI BINUR ADI PANCORO Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
description	Binur R, Pancoro A. 2017. Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia. Biodiversitas 18: 609-618. Inbreeding accumulation will tend to reduce genetic variation or depressed of the prawn fry produced. This problem has caused a decrease in production and quality of prawns culture in Indonesia. The purpose of this study is to measure the level of inbreeding depression prawn fry generated from several hatcheries in Java by microsatellite markers. There is four microsatellite locus to be used i.e Prk9A/T1, Prk4G/T1, TGFP16, and Mr8-88. The amplification of fourth locus using PCR with 6-carboxy-fluorescine (6-FAM) label. The number of alleles (Na) from fourth locus is Mr8-88 (11 alleles), TGFP16 (10 alleles), Prk4G/T1 (9 alleles), dan Prk9A/T1 (5 alleles), respectively. The level of polymorphism locus from highest to lowest is locus Prk4G/T1 (0.703), Prk9A/T1 (0.507), TGFP16 (0.410), and Mr8-88 (0.370), respectively. Inbreeding depression level of postlarvae (PL) M. rosenbergii tend to moderate with BBI Ciamis (He 0.444), BBUG Samas (He 0.514), LRPTBPAT Sukamandi (He 0.519), and UPBL Probolinggo (He 0.530), respectively. AMOVA analysis showed about 8.0% genetic variation among populations. From these results, it can be concluded that the PL produced indicated have been depressed. Post-larvae prawns produced from fourth hatcheries is not recommended to be a broodstock but can be used for cultivation for farmers.
format	article
author	ROBI BINUR ADI PANCORO
author_facet	ROBI BINUR ADI PANCORO
author_sort	ROBI BINUR
title	Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
title_short	Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
title_full	Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
title_fullStr	Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
title_full_unstemmed	Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia
title_sort	inbreeding depression level of post-larvae freshwater prawn (macrobrachium rosenbergii) from several hatcheries in java, indonesia
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/88518682ae4f4bd59ddb6b1b9504b498
work_keys_str_mv	AT robibinur inbreedingdepressionlevelofpostlarvaefreshwaterprawnmacrobrachiumrosenbergiifromseveralhatcheriesinjavaindonesia AT adipancoro inbreedingdepressionlevelofpostlarvaefreshwaterprawnmacrobrachiumrosenbergiifromseveralhatcheriesinjavaindonesia
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Inbreeding depression level of post-larvae freshwater prawn (Macrobrachium rosenbergii) from several hatcheries in Java, Indonesia

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