Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.

Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.

We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 p...

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Autores principales: Mikko Hölttä, Henrik Zetterberg, Ekaterina Mirgorodskaya, Niklas Mattsson, Kaj Blennow, Johan Gobom
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/8857f26691db425798f4acbee8a4347e
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spelling oai:doaj.org-article:8857f26691db425798f4acbee8a4347e2021-11-18T07:09:34ZPeptidome analysis of cerebrospinal fluid by LC-MALDI MS.1932-620310.1371/journal.pone.0042555https://doaj.org/article/8857f26691db425798f4acbee8a4347e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22880031/?tool=EBIhttps://doaj.org/toc/1932-6203We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.Mikko HölttäHenrik ZetterbergEkaterina MirgorodskayaNiklas MattssonKaj BlennowJohan GobomPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42555 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mikko Hölttä
Henrik Zetterberg
Ekaterina Mirgorodskaya
Niklas Mattsson
Kaj Blennow
Johan Gobom
Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
description We report on the analysis of endogenous peptides in cerebrospinal fluid (CSF) by mass spectrometry. A method was developed for preparation of peptide extracts from CSF. Analysis of the extracts by offline LC-MALDI MS resulted in the detection of 3,000-4,000 peptide-like features. Out of these, 730 peptides were identified by MS/MS. The majority of these peptides have not been previously reported in CSF. The identified peptides were found to originate from 104 proteins, of which several have been reported to be involved in different disorders of the central nervous system. These results support the notion that CSF peptidomics may be viable complement to proteomics in the search of biomarkers of CNS disorders.
format article
author Mikko Hölttä
Henrik Zetterberg
Ekaterina Mirgorodskaya
Niklas Mattsson
Kaj Blennow
Johan Gobom
author_facet Mikko Hölttä
Henrik Zetterberg
Ekaterina Mirgorodskaya
Niklas Mattsson
Kaj Blennow
Johan Gobom
author_sort Mikko Hölttä
title Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
title_short Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
title_full Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
title_fullStr Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
title_full_unstemmed Peptidome analysis of cerebrospinal fluid by LC-MALDI MS.
title_sort peptidome analysis of cerebrospinal fluid by lc-maldi ms.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/8857f26691db425798f4acbee8a4347e
work_keys_str_mv AT mikkoholtta peptidomeanalysisofcerebrospinalfluidbylcmaldims
AT henrikzetterberg peptidomeanalysisofcerebrospinalfluidbylcmaldims
AT ekaterinamirgorodskaya peptidomeanalysisofcerebrospinalfluidbylcmaldims
AT niklasmattsson peptidomeanalysisofcerebrospinalfluidbylcmaldims
AT kajblennow peptidomeanalysisofcerebrospinalfluidbylcmaldims
AT johangobom peptidomeanalysisofcerebrospinalfluidbylcmaldims
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