Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname

Abstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from...

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Autores principales:	Sander van Boheemen, Ali Tas, S. Yahya Anvar, Rebecca van Grootveld, Irina C. Albulescu, Martijn P. Bauer, Mariet C. Feltkamp, Peter J. Bredenbeek, Martijn J. van Hemert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/886636b805ea47d68fb3af0a6583c12b
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spelling	oai:doaj.org-article:886636b805ea47d68fb3af0a6583c12b2021-12-02T16:06:50ZQuasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname10.1038/s41598-017-02652-w2045-2322https://doaj.org/article/886636b805ea47d68fb3af0a6583c12b2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02652-whttps://doaj.org/toc/2045-2322Abstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5′ and 3′ untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10−4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate.Sander van BoheemenAli TasS. Yahya AnvarRebecca van GrootveldIrina C. AlbulescuMartijn P. BauerMariet C. FeltkampPeter J. BredenbeekMartijn J. van HemertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
description	Abstract The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5′ and 3′ untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10−4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate.
format	article
author	Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert
author_facet	Sander van Boheemen Ali Tas S. Yahya Anvar Rebecca van Grootveld Irina C. Albulescu Martijn P. Bauer Mariet C. Feltkamp Peter J. Bredenbeek Martijn J. van Hemert
author_sort	Sander van Boheemen
title	Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
title_short	Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
title_full	Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
title_fullStr	Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
title_full_unstemmed	Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname
title_sort	quasispecies composition and evolution of a typical zika virus clinical isolate from suriname
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/886636b805ea47d68fb3af0a6583c12b
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Quasispecies composition and evolution of a typical Zika virus clinical isolate from Suriname

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