Mycotoxin Fumonisin B1 Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks

Fumonisin B1 (FB1) is among the most common contaminants produced by Fusarium spp. fungus from corns and animal feeds. Although FB1 has been known to cause physical or functional defects of embryos in humans and...

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Autores principales:	Chompunut Lumsangkul, Ko-Hua Tso, Yang-Kwang Fan, Hsin-I Chiang, Jyh-Cherng Ju
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	duck embryos early embryogenesis fumonisin B<sub>1</sub> neural tube defects Medicine R
Acceso en línea:	https://doaj.org/article/886d4487da3c4f988256056c99af3555
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spelling	oai:doaj.org-article:886d4487da3c4f988256056c99af35552021-11-25T19:08:29ZMycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks10.3390/toxins131107432072-6651https://doaj.org/article/886d4487da3c4f988256056c99af35552021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/743https://doaj.org/toc/2072-6651Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is among the most common contaminants produced by <i>Fusarium</i> spp. fungus from corns and animal feeds. Although FB<sub>1</sub> has been known to cause physical or functional defects of embryos in humans and several animal species such as Syrian hamsters, rabbits, and rodents, little is known about the precise toxicity to the embryos and the underlying mechanisms have not been fully addressed. The present study aimed to investigate its developmental toxicity and potential mechanisms of action on sphingolipid metabolism in Brown Tsaiya Ducks (BTDs) embryos. We examined the effect of various FB<sub>1</sub> dosages (0, 10, 20 and 40 µg/embryo) on BTD embryogenesis 72 h post-incubation. The sphingomyelin content of duck embryos decreased (<i>p</i> < 0.05) in the highest FB<sub>1</sub>-treated group (40 µg). Failure of neural tube closure was observed in treated embryos and the expression levels of a neurulation-related gene, sonic hedgehog (<i>Shh</i>) was abnormally decreased. The sphingolipid metabolism-related genes including <i>N</i>-acylsphingosine amidohydrolase 1 (<i>ASAH1</i>), and ceramide synthase 6 (<i>CERS6</i>) expressions were altered in the treated embryos compared to those in the control embryos. Apparently, FB<sub>1</sub> have interfered sphingolipid metabolisms by inhibiting the functions of ceramide synthase and folate transporters. In conclusion, FB<sub>1</sub>-caused developmental retardation and abnormalities, such as neural tube defects in Brown Tsaiya Duck embryos, as well as are partly mediated by the disruption of sphingolipid metabolisms.Chompunut LumsangkulKo-Hua TsoYang-Kwang FanHsin-I ChiangJyh-Cherng JuMDPI AGarticleduck embryosearly embryogenesisfumonisin B<sub>1</sub>neural tube defectsMedicineRENToxins, Vol 13, Iss 743, p 743 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	duck embryos early embryogenesis fumonisin B<sub>1</sub> neural tube defects Medicine R
spellingShingle	duck embryos early embryogenesis fumonisin B<sub>1</sub> neural tube defects Medicine R Chompunut Lumsangkul Ko-Hua Tso Yang-Kwang Fan Hsin-I Chiang Jyh-Cherng Ju Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
description	Fumonisin B<sub>1</sub> (FB<sub>1</sub>) is among the most common contaminants produced by <i>Fusarium</i> spp. fungus from corns and animal feeds. Although FB<sub>1</sub> has been known to cause physical or functional defects of embryos in humans and several animal species such as Syrian hamsters, rabbits, and rodents, little is known about the precise toxicity to the embryos and the underlying mechanisms have not been fully addressed. The present study aimed to investigate its developmental toxicity and potential mechanisms of action on sphingolipid metabolism in Brown Tsaiya Ducks (BTDs) embryos. We examined the effect of various FB<sub>1</sub> dosages (0, 10, 20 and 40 µg/embryo) on BTD embryogenesis 72 h post-incubation. The sphingomyelin content of duck embryos decreased (<i>p</i> < 0.05) in the highest FB<sub>1</sub>-treated group (40 µg). Failure of neural tube closure was observed in treated embryos and the expression levels of a neurulation-related gene, sonic hedgehog (<i>Shh</i>) was abnormally decreased. The sphingolipid metabolism-related genes including <i>N</i>-acylsphingosine amidohydrolase 1 (<i>ASAH1</i>), and ceramide synthase 6 (<i>CERS6</i>) expressions were altered in the treated embryos compared to those in the control embryos. Apparently, FB<sub>1</sub> have interfered sphingolipid metabolisms by inhibiting the functions of ceramide synthase and folate transporters. In conclusion, FB<sub>1</sub>-caused developmental retardation and abnormalities, such as neural tube defects in Brown Tsaiya Duck embryos, as well as are partly mediated by the disruption of sphingolipid metabolisms.
format	article
author	Chompunut Lumsangkul Ko-Hua Tso Yang-Kwang Fan Hsin-I Chiang Jyh-Cherng Ju
author_facet	Chompunut Lumsangkul Ko-Hua Tso Yang-Kwang Fan Hsin-I Chiang Jyh-Cherng Ju
author_sort	Chompunut Lumsangkul
title	Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
title_short	Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
title_full	Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
title_fullStr	Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
title_full_unstemmed	Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks
title_sort	mycotoxin fumonisin b<sub>1</sub> interferes sphingolipid metabolisms and neural tube closure during early embryogenesis in brown tsaiya ducks
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/886d4487da3c4f988256056c99af3555
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Mycotoxin Fumonisin B<sub>1</sub> Interferes Sphingolipid Metabolisms and Neural Tube Closure during Early Embryogenesis in Brown Tsaiya Ducks

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