Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma

Background: aldolase A (ALDOA) has been reported to be involved in kinds of cancers. However, the role of ALDOA in lung adenocarcinoma has not been fully elucidated. In this study, we explored the prognostic value and correlation with immune infiltration of ALDOA in lung adenocarcinoma.Methods: The...

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Autores principales:	Guojun Lu, Wen Shi, Yu Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	lung adenocarcinoma AldoA biomarker prognosis immune infiltration Genetics QH426-470
Acceso en línea:	https://doaj.org/article/887212ef084d40b28372db276629e544
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spelling	oai:doaj.org-article:887212ef084d40b28372db276629e5442021-12-03T05:58:45ZPrognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma1664-802110.3389/fgene.2021.721021https://doaj.org/article/887212ef084d40b28372db276629e5442021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.721021/fullhttps://doaj.org/toc/1664-8021Background: aldolase A (ALDOA) has been reported to be involved in kinds of cancers. However, the role of ALDOA in lung adenocarcinoma has not been fully elucidated. In this study, we explored the prognostic value and correlation with immune infiltration of ALDOA in lung adenocarcinoma.Methods: The expression of ALDOA was analyzed with the Oncomine database, the Cancer Genome Atlas (TCGA), and the Human Protein Atlas (HPA). Mann-Whitney U test was performed to examine the relationship between clinicopathological characteristics and ALDOA expression. The receiver operating characteristic (ROC) curve and Kaplan-Meier method were conducted to describe the diagnostic and prognostic importance of ALDOA. The Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape were used to construct PPI networks and identify hub genes. Functional annotations and immune infiltration were conducted.Results: The mRNA and protein expression of ALDOA were higher in lung adenocarcinoma than those in normal tissues. The overexpression of ALDOA was significantly correlated with the high T stage, N stage, M stage, and TNM stage. Kaplan-Meier showed that high expression of ALDOA was correlated with short overall survival (38.9 vs 72.5 months, p < 0.001). Multivariate analysis revealed that ALDOA (HR 1.435, 95%CI, 1.013–2.032, p = 0.042) was an independent poor prognostic factor for overall survival. Functional enrichment analysis showed that positively co-expressed genes of ALDOA were involved in the biological progress of mitochondrial translation, mitochondrial translational elongation, and negative regulation of cell cycle progression. KEGG pathway analysis showed enrichment function in carbon metabolism, the HIF-1 signaling pathway, and glycolysis/gluconeogenesis. The “SCNA” module analysis indicated that the copy number alterations of ALDOA were correlated with three immune cell infiltration levels, including B cells, CD8+ T cells, and CD4+ T cells. The “Gene” module analysis indicated that ALDOA gene expression was negatively correlated with infiltrating levels of B cells, CD8+ T cells, CD4+ T cells, and macrophages.Conclusion: Our study suggested that upregulated ALDOA was significantly correlated with tumor progression, poor survival, and immune infiltrations in lung adenocarcinoma. These results suggest that ALDOA is a potential prognostic biomarker and therapeutic target in lung adenocarcinoma.Guojun LuWen ShiYu ZhangFrontiers Media S.A.articlelung adenocarcinomaAldoAbiomarkerprognosisimmune infiltrationGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lung adenocarcinoma AldoA biomarker prognosis immune infiltration Genetics QH426-470
spellingShingle	lung adenocarcinoma AldoA biomarker prognosis immune infiltration Genetics QH426-470 Guojun Lu Wen Shi Yu Zhang Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
description	Background: aldolase A (ALDOA) has been reported to be involved in kinds of cancers. However, the role of ALDOA in lung adenocarcinoma has not been fully elucidated. In this study, we explored the prognostic value and correlation with immune infiltration of ALDOA in lung adenocarcinoma.Methods: The expression of ALDOA was analyzed with the Oncomine database, the Cancer Genome Atlas (TCGA), and the Human Protein Atlas (HPA). Mann-Whitney U test was performed to examine the relationship between clinicopathological characteristics and ALDOA expression. The receiver operating characteristic (ROC) curve and Kaplan-Meier method were conducted to describe the diagnostic and prognostic importance of ALDOA. The Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape were used to construct PPI networks and identify hub genes. Functional annotations and immune infiltration were conducted.Results: The mRNA and protein expression of ALDOA were higher in lung adenocarcinoma than those in normal tissues. The overexpression of ALDOA was significantly correlated with the high T stage, N stage, M stage, and TNM stage. Kaplan-Meier showed that high expression of ALDOA was correlated with short overall survival (38.9 vs 72.5 months, p < 0.001). Multivariate analysis revealed that ALDOA (HR 1.435, 95%CI, 1.013–2.032, p = 0.042) was an independent poor prognostic factor for overall survival. Functional enrichment analysis showed that positively co-expressed genes of ALDOA were involved in the biological progress of mitochondrial translation, mitochondrial translational elongation, and negative regulation of cell cycle progression. KEGG pathway analysis showed enrichment function in carbon metabolism, the HIF-1 signaling pathway, and glycolysis/gluconeogenesis. The “SCNA” module analysis indicated that the copy number alterations of ALDOA were correlated with three immune cell infiltration levels, including B cells, CD8+ T cells, and CD4+ T cells. The “Gene” module analysis indicated that ALDOA gene expression was negatively correlated with infiltrating levels of B cells, CD8+ T cells, CD4+ T cells, and macrophages.Conclusion: Our study suggested that upregulated ALDOA was significantly correlated with tumor progression, poor survival, and immune infiltrations in lung adenocarcinoma. These results suggest that ALDOA is a potential prognostic biomarker and therapeutic target in lung adenocarcinoma.
format	article
author	Guojun Lu Wen Shi Yu Zhang
author_facet	Guojun Lu Wen Shi Yu Zhang
author_sort	Guojun Lu
title	Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
title_short	Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
title_full	Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
title_fullStr	Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
title_full_unstemmed	Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma
title_sort	prognostic implications and immune infiltration analysis of aldoa in lung adenocarcinoma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/887212ef084d40b28372db276629e544
work_keys_str_mv	AT guojunlu prognosticimplicationsandimmuneinfiltrationanalysisofaldoainlungadenocarcinoma AT wenshi prognosticimplicationsandimmuneinfiltrationanalysisofaldoainlungadenocarcinoma AT yuzhang prognosticimplicationsandimmuneinfiltrationanalysisofaldoainlungadenocarcinoma
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Prognostic Implications and Immune Infiltration Analysis of ALDOA in Lung Adenocarcinoma

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