Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability

The present study aimed to prepare a kind of controlled-releasing insulin-like growth factor 1 (IGF-1)/spider silk protein nanofibrous membrane using a electrostatic spinning method and evaluated its effect on the cell viability of endothelial progenitor cells (EPCs). Recombinant spidroin named as G...

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Autores principales:	Lifang Chen, Yulang Huang, Rongfeng Yang, Jian Xiao, Jiajia Gao, Debao Zhang, Duanwen Cao, Xiao Ke
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	controlled-releasing nanofibrous membrane degradation igf-1 endothelial progenitor cells tev protease Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/887d833be3474f648b1506af236b5a57
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spelling	oai:doaj.org-article:887d833be3474f648b1506af236b5a572021-11-11T14:23:43ZPreparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability2165-59792165-598710.1080/21655979.2021.1982270https://doaj.org/article/887d833be3474f648b1506af236b5a572021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982270https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987The present study aimed to prepare a kind of controlled-releasing insulin-like growth factor 1 (IGF-1)/spider silk protein nanofibrous membrane using a electrostatic spinning method and evaluated its effect on the cell viability of endothelial progenitor cells (EPCs). Recombinant spidroin named as GMCDRSSP-IgF-1 was electro-spun into nanofibrous membrane which can be degraded by protease and be capable of sustained-release of IGF-1. The membrane can be degraded after being treated with thrombin. The release assay results showed that IGF-1 concentration could be maintained at 20 ng/ml for a long time with treatment of Tobacco Etch Virus (TEV) protease. The viability of EPCs on GMCDRSSP-IgF-1 nanofibrous membrane was significantly increased with the presence of TEV protease. The controlled and sustained release of IGF-1 from the nanofibrous membrane could promote the adhesion and viability of EPCs. In summary, the nanofibrous membrane that exhibits controlled degradation and sustained release of IGF-1 was prepared with electrostatic spinning from genetically modified recombinant spider silk protein. The nanofibrous membrane exhibited good blood compatibility and cytocompatibility. With the presence of TEV protease, the sustained-release of IGF-1 significantly promoted the adhesion and viability of EPCs. The new nanofibrous membrane can be potentially used as a scaffold for EPCs culture in vitro and future in vivo studies.Lifang ChenYulang HuangRongfeng YangJian XiaoJiajia GaoDebao ZhangDuanwen CaoXiao KeTaylor & Francis Grouparticlecontrolled-releasingnanofibrous membranedegradationigf-1endothelial progenitor cellstev proteaseBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8031-8042 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	controlled-releasing nanofibrous membrane degradation igf-1 endothelial progenitor cells tev protease Biotechnology TP248.13-248.65
spellingShingle	controlled-releasing nanofibrous membrane degradation igf-1 endothelial progenitor cells tev protease Biotechnology TP248.13-248.65 Lifang Chen Yulang Huang Rongfeng Yang Jian Xiao Jiajia Gao Debao Zhang Duanwen Cao Xiao Ke Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
description	The present study aimed to prepare a kind of controlled-releasing insulin-like growth factor 1 (IGF-1)/spider silk protein nanofibrous membrane using a electrostatic spinning method and evaluated its effect on the cell viability of endothelial progenitor cells (EPCs). Recombinant spidroin named as GMCDRSSP-IgF-1 was electro-spun into nanofibrous membrane which can be degraded by protease and be capable of sustained-release of IGF-1. The membrane can be degraded after being treated with thrombin. The release assay results showed that IGF-1 concentration could be maintained at 20 ng/ml for a long time with treatment of Tobacco Etch Virus (TEV) protease. The viability of EPCs on GMCDRSSP-IgF-1 nanofibrous membrane was significantly increased with the presence of TEV protease. The controlled and sustained release of IGF-1 from the nanofibrous membrane could promote the adhesion and viability of EPCs. In summary, the nanofibrous membrane that exhibits controlled degradation and sustained release of IGF-1 was prepared with electrostatic spinning from genetically modified recombinant spider silk protein. The nanofibrous membrane exhibited good blood compatibility and cytocompatibility. With the presence of TEV protease, the sustained-release of IGF-1 significantly promoted the adhesion and viability of EPCs. The new nanofibrous membrane can be potentially used as a scaffold for EPCs culture in vitro and future in vivo studies.
format	article
author	Lifang Chen Yulang Huang Rongfeng Yang Jian Xiao Jiajia Gao Debao Zhang Duanwen Cao Xiao Ke
author_facet	Lifang Chen Yulang Huang Rongfeng Yang Jian Xiao Jiajia Gao Debao Zhang Duanwen Cao Xiao Ke
author_sort	Lifang Chen
title	Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
title_short	Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
title_full	Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
title_fullStr	Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
title_full_unstemmed	Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
title_sort	preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/887d833be3474f648b1506af236b5a57
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Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability

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