Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa

Abstract Multi-drug (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) continues to be a global public health problem especially in high TB burden countries like Nigeria. Many of these cases are undetected and go on to infect high risk individuals. Clinical samples from positive rifampicin...

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Autores principales:	Idowu B. Olawoye, Jessica N. Uwanibe, Chioma N. Kunle-Ope, Olabisi F. Davies-Bolorunduro, Temitope A. Abiodun, Rosemary A. Audu, Babatunde L. Salako, Christian T. Happi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/887ee0fc876b4a20847fa371579068f5
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spelling	oai:doaj.org-article:887ee0fc876b4a20847fa371579068f52021-12-02T19:09:30ZWhole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa10.1038/s41598-021-96956-72045-2322https://doaj.org/article/887ee0fc876b4a20847fa371579068f52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96956-7https://doaj.org/toc/2045-2322Abstract Multi-drug (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) continues to be a global public health problem especially in high TB burden countries like Nigeria. Many of these cases are undetected and go on to infect high risk individuals. Clinical samples from positive rifampicin resistant Xpert®MTB/Rif assay were subjected to direct whole genome sequencing and bioinformatics analysis to identify the full antibiotics resistance and lineage profile. We report two (2) XDR TB samples also belonging to the East-Asian/Beijing family of lineage 2 Mycobacterium tuberculosis complex from clinical samples in Nigeria. Our findings further reveal the presence of mutations that confer resistance to first-line drugs (rifampicin, isoniazid, ethambutol and pyrazanimide), second-line injectables (capreomycin, streptomycin, kanamycin and/or amikacin) and at least one of the fluoroquinolones (ofloxacin, moxifloxacin, levofloxacin and/or ciprofloxacin) in both samples. The genomic sequence data from this study not only provide the first evidence of XDR TB in Nigeria and West Africa, but also emphasize the importance of WGS in accurately detecting MDR and XDR TB, to ensure adequate and proper management treatment regimens for affected individuals. This will greatly aid in preventing the spread of drug resistance TB in high burden countries.Idowu B. OlawoyeJessica N. UwanibeChioma N. Kunle-OpeOlabisi F. Davies-BolorunduroTemitope A. AbiodunRosemary A. AuduBabatunde L. SalakoChristian T. HappiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Idowu B. Olawoye Jessica N. Uwanibe Chioma N. Kunle-Ope Olabisi F. Davies-Bolorunduro Temitope A. Abiodun Rosemary A. Audu Babatunde L. Salako Christian T. Happi Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
description	Abstract Multi-drug (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) continues to be a global public health problem especially in high TB burden countries like Nigeria. Many of these cases are undetected and go on to infect high risk individuals. Clinical samples from positive rifampicin resistant Xpert®MTB/Rif assay were subjected to direct whole genome sequencing and bioinformatics analysis to identify the full antibiotics resistance and lineage profile. We report two (2) XDR TB samples also belonging to the East-Asian/Beijing family of lineage 2 Mycobacterium tuberculosis complex from clinical samples in Nigeria. Our findings further reveal the presence of mutations that confer resistance to first-line drugs (rifampicin, isoniazid, ethambutol and pyrazanimide), second-line injectables (capreomycin, streptomycin, kanamycin and/or amikacin) and at least one of the fluoroquinolones (ofloxacin, moxifloxacin, levofloxacin and/or ciprofloxacin) in both samples. The genomic sequence data from this study not only provide the first evidence of XDR TB in Nigeria and West Africa, but also emphasize the importance of WGS in accurately detecting MDR and XDR TB, to ensure adequate and proper management treatment regimens for affected individuals. This will greatly aid in preventing the spread of drug resistance TB in high burden countries.
format	article
author	Idowu B. Olawoye Jessica N. Uwanibe Chioma N. Kunle-Ope Olabisi F. Davies-Bolorunduro Temitope A. Abiodun Rosemary A. Audu Babatunde L. Salako Christian T. Happi
author_facet	Idowu B. Olawoye Jessica N. Uwanibe Chioma N. Kunle-Ope Olabisi F. Davies-Bolorunduro Temitope A. Abiodun Rosemary A. Audu Babatunde L. Salako Christian T. Happi
author_sort	Idowu B. Olawoye
title	Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
title_short	Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
title_full	Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
title_fullStr	Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
title_full_unstemmed	Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa
title_sort	whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (xdr tb) strains from the beijing lineage in nigeria, west africa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/887ee0fc876b4a20847fa371579068f5
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Whole genome sequencing of clinical samples reveals extensively drug resistant tuberculosis (XDR TB) strains from the Beijing lineage in Nigeria, West Africa

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