Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12

Abstract Due to their capacity to skew T cell responses towards Th1 oriented immunity, oligonucleotides containing unmethylated CpG motifs (CpG) have emerged as interesting adjuvants for vaccination. Whereas the signalling pathways in response to CpG mediated TLR9 activation have been extensively do...

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Autores principales:	S. De Koker, L. Van Hoecke, A. De Beuckelaer, K. Roose, K. Deswarte, M. A. Willart, P. Bogaert, T. Naessens, B. G. De Geest, X. Saelens, B. N. Lambrecht, J. Grooten
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88807313776544b8b57f2db47992b929
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spelling	oai:doaj.org-article:88807313776544b8b57f2db47992b9292021-12-02T16:08:21ZInflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-1210.1038/s41598-017-06236-62045-2322https://doaj.org/article/88807313776544b8b57f2db47992b9292017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06236-6https://doaj.org/toc/2045-2322Abstract Due to their capacity to skew T cell responses towards Th1 oriented immunity, oligonucleotides containing unmethylated CpG motifs (CpG) have emerged as interesting adjuvants for vaccination. Whereas the signalling pathways in response to CpG mediated TLR9 activation have been extensively documented at the level of the individual cell, little is however known on the precise identity of the innate immune cells that govern T cell priming and polarisation to CpG adjuvanted protein antigens in vivo. In this study, we demonstrate that optimal induction of Th1 oriented immunity to CpG adjuvanted protein vaccines requires the coordinated actions of conventional DCs and of monocytes. Whilst conventional DCs were required for antigen presentation and initial T cell priming, monocytes constitute the main source of the Th1 polarising cytokine IL-12.S. De KokerL. Van HoeckeA. De BeuckelaerK. RooseK. DeswarteM. A. WillartP. BogaertT. NaessensB. G. De GeestX. SaelensB. N. LambrechtJ. GrootenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. De Koker L. Van Hoecke A. De Beuckelaer K. Roose K. Deswarte M. A. Willart P. Bogaert T. Naessens B. G. De Geest X. Saelens B. N. Lambrecht J. Grooten Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
description	Abstract Due to their capacity to skew T cell responses towards Th1 oriented immunity, oligonucleotides containing unmethylated CpG motifs (CpG) have emerged as interesting adjuvants for vaccination. Whereas the signalling pathways in response to CpG mediated TLR9 activation have been extensively documented at the level of the individual cell, little is however known on the precise identity of the innate immune cells that govern T cell priming and polarisation to CpG adjuvanted protein antigens in vivo. In this study, we demonstrate that optimal induction of Th1 oriented immunity to CpG adjuvanted protein vaccines requires the coordinated actions of conventional DCs and of monocytes. Whilst conventional DCs were required for antigen presentation and initial T cell priming, monocytes constitute the main source of the Th1 polarising cytokine IL-12.
format	article
author	S. De Koker L. Van Hoecke A. De Beuckelaer K. Roose K. Deswarte M. A. Willart P. Bogaert T. Naessens B. G. De Geest X. Saelens B. N. Lambrecht J. Grooten
author_facet	S. De Koker L. Van Hoecke A. De Beuckelaer K. Roose K. Deswarte M. A. Willart P. Bogaert T. Naessens B. G. De Geest X. Saelens B. N. Lambrecht J. Grooten
author_sort	S. De Koker
title	Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
title_short	Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
title_full	Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
title_fullStr	Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
title_full_unstemmed	Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12
title_sort	inflammatory monocytes regulate th1 oriented immunity to cpg adjuvanted protein vaccines through production of il-12
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/88807313776544b8b57f2db47992b929
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Inflammatory monocytes regulate Th1 oriented immunity to CpG adjuvanted protein vaccines through production of IL-12

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