Functional analysis of the ComK protein of Bacillus coagulans.

The genes for DNA uptake and recombination in Bacilli are commonly regulated by the transcriptional factor ComK. We have identified a ComK homologue in Bacillus coagulans, an industrial relevant organism that is recalcitrant for transformation. Introduction of B. coagulans comK gene under its own pr...

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Autores principales:	Ákos T Kovács, Tom H Eckhardt, Mariska van Hartskamp, Richard van Kranenburg, Oscar P Kuipers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88826e4dcd6c4f6f89aff9e64a6110e3
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spelling	oai:doaj.org-article:88826e4dcd6c4f6f89aff9e64a6110e32021-11-18T08:02:44ZFunctional analysis of the ComK protein of Bacillus coagulans.1932-620310.1371/journal.pone.0053471https://doaj.org/article/88826e4dcd6c4f6f89aff9e64a6110e32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23301076/?tool=EBIhttps://doaj.org/toc/1932-6203The genes for DNA uptake and recombination in Bacilli are commonly regulated by the transcriptional factor ComK. We have identified a ComK homologue in Bacillus coagulans, an industrial relevant organism that is recalcitrant for transformation. Introduction of B. coagulans comK gene under its own promoter region into Bacillus subtilis comK strain results in low transcriptional induction of the late competence gene comGA, but lacking bistable expression. The promoter regions of B. coagulans comK and the comGA genes are recognized in B. subtilis and expression from these promoters is activated by B. subtilis ComK. Purified ComK protein of B. coagulans showed DNA-binding ability in gel retardation assays with B. subtilis- and B. coagulans-derived probes. These experiments suggest that the function of B. coagulans ComK is similar to that of ComK of B. subtilis. When its own comK is overexpressed in B. coagulans the comGA gene expression increases 40-fold, while the expression of another late competence gene, comC is not elevated and no reproducible DNA-uptake could be observed under these conditions. Our results demonstrate that B. coagulans ComK can recognize several B.subtilis comK-responsive elements, and vice versa, but indicate that the activation of the transcription of complete sets of genes coding for a putative DNA uptake apparatus in B. coagulans might differ from that of B. subtilis.Ákos T KovácsTom H EckhardtMariska van HartskampRichard van KranenburgOscar P KuipersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53471 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ákos T Kovács Tom H Eckhardt Mariska van Hartskamp Richard van Kranenburg Oscar P Kuipers Functional analysis of the ComK protein of Bacillus coagulans.
description	The genes for DNA uptake and recombination in Bacilli are commonly regulated by the transcriptional factor ComK. We have identified a ComK homologue in Bacillus coagulans, an industrial relevant organism that is recalcitrant for transformation. Introduction of B. coagulans comK gene under its own promoter region into Bacillus subtilis comK strain results in low transcriptional induction of the late competence gene comGA, but lacking bistable expression. The promoter regions of B. coagulans comK and the comGA genes are recognized in B. subtilis and expression from these promoters is activated by B. subtilis ComK. Purified ComK protein of B. coagulans showed DNA-binding ability in gel retardation assays with B. subtilis- and B. coagulans-derived probes. These experiments suggest that the function of B. coagulans ComK is similar to that of ComK of B. subtilis. When its own comK is overexpressed in B. coagulans the comGA gene expression increases 40-fold, while the expression of another late competence gene, comC is not elevated and no reproducible DNA-uptake could be observed under these conditions. Our results demonstrate that B. coagulans ComK can recognize several B.subtilis comK-responsive elements, and vice versa, but indicate that the activation of the transcription of complete sets of genes coding for a putative DNA uptake apparatus in B. coagulans might differ from that of B. subtilis.
format	article
author	Ákos T Kovács Tom H Eckhardt Mariska van Hartskamp Richard van Kranenburg Oscar P Kuipers
author_facet	Ákos T Kovács Tom H Eckhardt Mariska van Hartskamp Richard van Kranenburg Oscar P Kuipers
author_sort	Ákos T Kovács
title	Functional analysis of the ComK protein of Bacillus coagulans.
title_short	Functional analysis of the ComK protein of Bacillus coagulans.
title_full	Functional analysis of the ComK protein of Bacillus coagulans.
title_fullStr	Functional analysis of the ComK protein of Bacillus coagulans.
title_full_unstemmed	Functional analysis of the ComK protein of Bacillus coagulans.
title_sort	functional analysis of the comk protein of bacillus coagulans.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/88826e4dcd6c4f6f89aff9e64a6110e3
work_keys_str_mv	AT akostkovacs functionalanalysisofthecomkproteinofbacilluscoagulans AT tomheckhardt functionalanalysisofthecomkproteinofbacilluscoagulans AT mariskavanhartskamp functionalanalysisofthecomkproteinofbacilluscoagulans AT richardvankranenburg functionalanalysisofthecomkproteinofbacilluscoagulans AT oscarpkuipers functionalanalysisofthecomkproteinofbacilluscoagulans
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Functional analysis of the ComK protein of Bacillus coagulans.

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