Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing

Severe acute respiratory syndrome coronavirus 2 is an RNA virus currently causing a pandemic. Due to errors during replication, mutations can occur and result in cell adaptation by the virus or in the rise of new variants. This can change the attachment receptors' usage, result in different mor...

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Autores principales:	Gregory Mathez, Valeria Cagno
Formato:	article
Lenguaje:	EN
Publicado:	SAGE Publishing 2021
Materias:	Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/888abe7d857f46f1bfc1677795b6f372
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spelling	oai:doaj.org-article:888abe7d857f46f1bfc1677795b6f3722021-11-21T01:33:40ZClinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing2040-206610.1177/20402066211061063https://doaj.org/article/888abe7d857f46f1bfc1677795b6f3722021-11-01T00:00:00Zhttps://doi.org/10.1177/20402066211061063https://doaj.org/toc/2040-2066Severe acute respiratory syndrome coronavirus 2 is an RNA virus currently causing a pandemic. Due to errors during replication, mutations can occur and result in cell adaptation by the virus or in the rise of new variants. This can change the attachment receptors' usage, result in different morphology of plaques, and can affect as well antiviral development. Indeed, a molecule can be active on laboratory strains but not necessarily on circulating strains or be effective only against some viral variants. Experiments with clinical samples with limited cell adaptation should be performed to confirm the efficiency of drugs of interest. In this protocol, we present a method to culture severe acute respiratory syndrome coronavirus 2 from nasopharyngeal swabs, obtain a high viral titer while limiting cell adaptation, and assess antiviral efficiency.Gregory MathezValeria CagnoSAGE PublishingarticleInfectious and parasitic diseasesRC109-216ENAntiviral Chemistry & Chemotherapy, Vol 29 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Infectious and parasitic diseases RC109-216
spellingShingle	Infectious and parasitic diseases RC109-216 Gregory Mathez Valeria Cagno Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
description	Severe acute respiratory syndrome coronavirus 2 is an RNA virus currently causing a pandemic. Due to errors during replication, mutations can occur and result in cell adaptation by the virus or in the rise of new variants. This can change the attachment receptors' usage, result in different morphology of plaques, and can affect as well antiviral development. Indeed, a molecule can be active on laboratory strains but not necessarily on circulating strains or be effective only against some viral variants. Experiments with clinical samples with limited cell adaptation should be performed to confirm the efficiency of drugs of interest. In this protocol, we present a method to culture severe acute respiratory syndrome coronavirus 2 from nasopharyngeal swabs, obtain a high viral titer while limiting cell adaptation, and assess antiviral efficiency.
format	article
author	Gregory Mathez Valeria Cagno
author_facet	Gregory Mathez Valeria Cagno
author_sort	Gregory Mathez
title	Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
title_short	Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
title_full	Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
title_fullStr	Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
title_full_unstemmed	Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
title_sort	clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing
publisher	SAGE Publishing
publishDate	2021
url	https://doaj.org/article/888abe7d857f46f1bfc1677795b6f372
work_keys_str_mv	AT gregorymathez clinicalsevereacuterespiratorysyndromecoronavirus2isolationandantiviraltesting AT valeriacagno clinicalsevereacuterespiratorysyndromecoronavirus2isolationandantiviraltesting
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Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing

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