Virus versus host plant microRNAs: who determines the outcome of the interaction?

Considering the importance of microRNAs (miRNAs) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant miRNA sequences counteract viral attack via antiviral RNA silencing, viruses in turn have developed antihost defense mechanisms blocking th...

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Autores principales:	Fatemeh Maghuly, Rose C Ramkat, Margit Laimer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/888ea61b97d54c8d9c328da8d0742eaa
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spelling	oai:doaj.org-article:888ea61b97d54c8d9c328da8d0742eaa2021-11-18T08:17:16ZVirus versus host plant microRNAs: who determines the outcome of the interaction?1932-620310.1371/journal.pone.0098263https://doaj.org/article/888ea61b97d54c8d9c328da8d0742eaa2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24896088/?tool=EBIhttps://doaj.org/toc/1932-6203Considering the importance of microRNAs (miRNAs) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant miRNA sequences counteract viral attack via antiviral RNA silencing, viruses in turn have developed antihost defense mechanisms blocking these RNA silencing pathways and establish a counter-defense. In the current study, computational and stem-loop Reverse Transcription - Polymerase Chain Reaction (RT-PCR) approaches were employed to a) predict and validate virus encoded mature miRNAs (miRs) in 39 DNA-A sequences of the bipartite genomes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus-Uganda (EACMV-UG) isolates, b) determine whether virus encoded miRs/miRs* generated from the 5'/3' harpin arms have the capacity to bind to genomic sequences of the host plants Jatropha or cassava and c) investigate whether plant encoded miR/miR* sequences have the potential to bind to the viral genomes. Different viral pre-miRNA hairpin sequences and viral miR/miR* length variants occurring as isomiRs were predicted in both viruses. These miRNAs were located in three Open Reading Frames (ORFs) and in the Intergenic Region (IR). Moreover, various target genes for miRNAs from both viruses were predicted and annotated in the host plant genomes indicating that they are involved in biotic response, metabolic pathways and transcription factors. Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense. This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts. These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.Fatemeh MaghulyRose C RamkatMargit LaimerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e98263 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fatemeh Maghuly Rose C Ramkat Margit Laimer Virus versus host plant microRNAs: who determines the outcome of the interaction?
description	Considering the importance of microRNAs (miRNAs) in the regulation of essential processes in plant pathogen interactions, it is not surprising that, while plant miRNA sequences counteract viral attack via antiviral RNA silencing, viruses in turn have developed antihost defense mechanisms blocking these RNA silencing pathways and establish a counter-defense. In the current study, computational and stem-loop Reverse Transcription - Polymerase Chain Reaction (RT-PCR) approaches were employed to a) predict and validate virus encoded mature miRNAs (miRs) in 39 DNA-A sequences of the bipartite genomes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus-Uganda (EACMV-UG) isolates, b) determine whether virus encoded miRs/miRs* generated from the 5'/3' harpin arms have the capacity to bind to genomic sequences of the host plants Jatropha or cassava and c) investigate whether plant encoded miR/miR* sequences have the potential to bind to the viral genomes. Different viral pre-miRNA hairpin sequences and viral miR/miR* length variants occurring as isomiRs were predicted in both viruses. These miRNAs were located in three Open Reading Frames (ORFs) and in the Intergenic Region (IR). Moreover, various target genes for miRNAs from both viruses were predicted and annotated in the host plant genomes indicating that they are involved in biotic response, metabolic pathways and transcription factors. Plant miRs/miRs* from conserved and highly expressed families were identified, which were shown to have potential targets in the genome of both begomoviruses, representing potential plant miRNAs mediating antiviral defense. This is the first assessment of predicted viral miRs/miRs* of ACMV and EACMV-UG and host plant miRNAs, providing a reference point for miRNA identification in pathogens and their hosts. These findings will improve the understanding of host- pathogen interaction pathways and the function of viral miRNAs in Euphorbiaceous crop plants.
format	article
author	Fatemeh Maghuly Rose C Ramkat Margit Laimer
author_facet	Fatemeh Maghuly Rose C Ramkat Margit Laimer
author_sort	Fatemeh Maghuly
title	Virus versus host plant microRNAs: who determines the outcome of the interaction?
title_short	Virus versus host plant microRNAs: who determines the outcome of the interaction?
title_full	Virus versus host plant microRNAs: who determines the outcome of the interaction?
title_fullStr	Virus versus host plant microRNAs: who determines the outcome of the interaction?
title_full_unstemmed	Virus versus host plant microRNAs: who determines the outcome of the interaction?
title_sort	virus versus host plant micrornas: who determines the outcome of the interaction?
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/888ea61b97d54c8d9c328da8d0742eaa
work_keys_str_mv	AT fatemehmaghuly virusversushostplantmicrornaswhodeterminestheoutcomeoftheinteraction AT rosecramkat virusversushostplantmicrornaswhodeterminestheoutcomeoftheinteraction AT margitlaimer virusversushostplantmicrornaswhodeterminestheoutcomeoftheinteraction
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Virus versus host plant microRNAs: who determines the outcome of the interaction?

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