Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy

Sebastian Aguayo,1 Nikolaos Donos,2 Dave Spratt,3 Laurent Bozec11Department of Biomaterials and Tissue Engineering, 2Periodontology Unit, 3Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UKAbstract: As titanium (Ti) continues to be utilized i...

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Autores principales:	Aguayo S, Donos N, Spratt D, Bozec L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8891a501e604426c9e1993d76d4d55ff
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spelling	oai:doaj.org-article:8891a501e604426c9e1993d76d4d55ff2021-12-02T05:09:55ZProbing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy1178-2013https://doaj.org/article/8891a501e604426c9e1993d76d4d55ff2016-04-01T00:00:00Zhttps://www.dovepress.com/probing-the-nanoadhesion-of-streptococcus-sanguinis-to-titanium-implan-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sebastian Aguayo,1 Nikolaos Donos,2 Dave Spratt,3 Laurent Bozec11Department of Biomaterials and Tissue Engineering, 2Periodontology Unit, 3Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UKAbstract: As titanium (Ti) continues to be utilized in great extent for the fabrication of artificial implants, it is important to understand the crucial bacterium–Ti interaction occurring during the initial phases of biofilm formation. By employing a single-cell force spectroscopy technique, the nanoadhesive interactions between the early-colonizing Streptococcus sanguinis and a clinically analogous smooth Ti substrate were explored. Mean adhesion forces between S. sanguinis and Ti were found to be 0.32±0.00, 1.07±0.06, and 4.85±0.56 nN for 0, 1, and 60 seconds contact times, respectively; while adhesion work values were reported at 19.28±2.38, 104.60±7.02, and 1,317.26±197.69 aJ for 0, 1, and 60 seconds, respectively. At 60 seconds surface delays, minor-rupture events were modeled with the worm-like chain model yielding an average contour length of 668±12 nm. The mean force for S. sanguinis minor-detachment events was 1.84±0.64 nN, and Poisson analysis decoupled this value into a short-range force component of -1.60±0.34 nN and a long-range force component of -0.55±0.47 nN. Furthermore, a solution of 2 mg/mL chlorhexidine was found to increase adhesion between the bacterial probe and substrate. Overall, single-cell force spectroscopy of living S. sanguinis cells proved to be a reliable way to characterize early-bacterial adhesion onto machined Ti implant surfaces at the nanoscale.Keywords: atomic force microscopy, biophysics, bacterial adhesion, dental implants, titaniumAguayo SDonos NSpratt DBozec LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1443-1450 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Aguayo S Donos N Spratt D Bozec L Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
description	Sebastian Aguayo,1 Nikolaos Donos,2 Dave Spratt,3 Laurent Bozec11Department of Biomaterials and Tissue Engineering, 2Periodontology Unit, 3Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, London, UKAbstract: As titanium (Ti) continues to be utilized in great extent for the fabrication of artificial implants, it is important to understand the crucial bacterium–Ti interaction occurring during the initial phases of biofilm formation. By employing a single-cell force spectroscopy technique, the nanoadhesive interactions between the early-colonizing Streptococcus sanguinis and a clinically analogous smooth Ti substrate were explored. Mean adhesion forces between S. sanguinis and Ti were found to be 0.32±0.00, 1.07±0.06, and 4.85±0.56 nN for 0, 1, and 60 seconds contact times, respectively; while adhesion work values were reported at 19.28±2.38, 104.60±7.02, and 1,317.26±197.69 aJ for 0, 1, and 60 seconds, respectively. At 60 seconds surface delays, minor-rupture events were modeled with the worm-like chain model yielding an average contour length of 668±12 nm. The mean force for S. sanguinis minor-detachment events was 1.84±0.64 nN, and Poisson analysis decoupled this value into a short-range force component of -1.60±0.34 nN and a long-range force component of -0.55±0.47 nN. Furthermore, a solution of 2 mg/mL chlorhexidine was found to increase adhesion between the bacterial probe and substrate. Overall, single-cell force spectroscopy of living S. sanguinis cells proved to be a reliable way to characterize early-bacterial adhesion onto machined Ti implant surfaces at the nanoscale.Keywords: atomic force microscopy, biophysics, bacterial adhesion, dental implants, titanium
format	article
author	Aguayo S Donos N Spratt D Bozec L
author_facet	Aguayo S Donos N Spratt D Bozec L
author_sort	Aguayo S
title	Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
title_short	Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
title_full	Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
title_fullStr	Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
title_full_unstemmed	Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
title_sort	probing the nanoadhesion of streptococcus sanguinis to titanium implant surfaces by atomic force microscopy
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/8891a501e604426c9e1993d76d4d55ff
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Probing the nanoadhesion of Streptococcus sanguinis to titanium implant surfaces by atomic force microscopy

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