Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus

Haiyan Jiao,1,* Zhiyi Yan,1,* Qingyu Ma,2 Xiaojuan Li,2 Youming Jiang,1 Yueyun Liu,1 Jiaxu Chen1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; 2Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guan...

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Autores principales:	Jiao H, Yan Z, Ma Q, Li X, Jiang Y, Liu Y, Chen J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Xiaoyaosan Depression Chronic immobilization stress Tryptophan TPH2 IDO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/88948e5a1ef94c8ab816aa1dac32727d
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spelling	oai:doaj.org-article:88948e5a1ef94c8ab816aa1dac32727d2021-12-02T07:25:42ZInfluence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus1178-2021https://doaj.org/article/88948e5a1ef94c8ab816aa1dac32727d2018-12-01T00:00:00Zhttps://www.dovepress.com/influence-of-xiaoyaosan-on-depressive-like-behaviors-in-chronic-stress-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Haiyan Jiao,1,* Zhiyi Yan,1,* Qingyu Ma,2 Xiaojuan Li,2 Youming Jiang,1 Yueyun Liu,1 Jiaxu Chen1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; 2Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China *These authors contributed equally to this work Background: Tryptophan metabolism has always been considered to play a vital role in mental disorder diseases, and how traditional Chinese formula Xiaoyaosan regulates the tryptophan metabolism is a complement to the pathogenesis of depression. This study established a depression rat model by the chronic immobilization stress (CIS) method and observed the change in tryptophan metabolism in hippocampus and the effects of Xiaoyaosan.Methods: Forty-eight male Sprague Dawley (SD) rats were randomly divided into the following four groups: control group, CIS group, Xiaoyaosan group, and fluoxetine group. The depression model was established by the 21-day CIS. The food intake and body weight were recorded, and the sucrose preference test (SPT), novelty suppressed feeding (NSF) test and open field test (OFT) were also used to evaluate the model. Then, the contents of tryptophan and 5-hydroxytryptamine (5-HT) in hippocampus were detected by the ELISA method, and the expression levels of tryptophan hydrogenase 2 (TPH2) and indoleamine 2,3-dioxygenase 1 (IDO1) in hippocampus were determined by quantitative reverse transcriptase polymerase chain reaction reaction (qRT-PCR) and Western blot methods.Results: The behavioral data showed a significant difference between the model group and the normal group. The 5-HT content in the hippocampi of CIS rats was significantly reduced, whereas the tryptophan content in the hippocampi of model rats was significantly increased. The TPH2 level in hippocampus of the model group was significantly decreased, and the IDO1 level was significantly increased. Xiaoyaosan and fluoxetine could significantly reverse these changes and had obvious curative effects.Conclusion: The abnormal tryptophan metabolism existed in the hippocampi of chronic stress-depressed rats, which was closely related to the pathogenesis of depression. Xiaoyaosan could improve the tryptophan metabolism by regulating the expression levels of TPH2 and IDO1, thus exerting an antidepressant-like effect. Keywords: Xiaoyaosan, depression, chronic immobilization stress, tryptophan, TPH2, IDO1, traditional Chinese formula, traditional Chinese medicine Jiao HYan ZMa QLi XJiang YLiu YChen JDove Medical PressarticleXiaoyaosanDepressionChronic immobilization stressTryptophanTPH2IDO1Neurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 21-31 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Xiaoyaosan Depression Chronic immobilization stress Tryptophan TPH2 IDO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Xiaoyaosan Depression Chronic immobilization stress Tryptophan TPH2 IDO1 Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Jiao H Yan Z Ma Q Li X Jiang Y Liu Y Chen J Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
description	Haiyan Jiao,1,* Zhiyi Yan,1,* Qingyu Ma,2 Xiaojuan Li,2 Youming Jiang,1 Yueyun Liu,1 Jiaxu Chen1,2 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China; 2Formula-Pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China *These authors contributed equally to this work Background: Tryptophan metabolism has always been considered to play a vital role in mental disorder diseases, and how traditional Chinese formula Xiaoyaosan regulates the tryptophan metabolism is a complement to the pathogenesis of depression. This study established a depression rat model by the chronic immobilization stress (CIS) method and observed the change in tryptophan metabolism in hippocampus and the effects of Xiaoyaosan.Methods: Forty-eight male Sprague Dawley (SD) rats were randomly divided into the following four groups: control group, CIS group, Xiaoyaosan group, and fluoxetine group. The depression model was established by the 21-day CIS. The food intake and body weight were recorded, and the sucrose preference test (SPT), novelty suppressed feeding (NSF) test and open field test (OFT) were also used to evaluate the model. Then, the contents of tryptophan and 5-hydroxytryptamine (5-HT) in hippocampus were detected by the ELISA method, and the expression levels of tryptophan hydrogenase 2 (TPH2) and indoleamine 2,3-dioxygenase 1 (IDO1) in hippocampus were determined by quantitative reverse transcriptase polymerase chain reaction reaction (qRT-PCR) and Western blot methods.Results: The behavioral data showed a significant difference between the model group and the normal group. The 5-HT content in the hippocampi of CIS rats was significantly reduced, whereas the tryptophan content in the hippocampi of model rats was significantly increased. The TPH2 level in hippocampus of the model group was significantly decreased, and the IDO1 level was significantly increased. Xiaoyaosan and fluoxetine could significantly reverse these changes and had obvious curative effects.Conclusion: The abnormal tryptophan metabolism existed in the hippocampi of chronic stress-depressed rats, which was closely related to the pathogenesis of depression. Xiaoyaosan could improve the tryptophan metabolism by regulating the expression levels of TPH2 and IDO1, thus exerting an antidepressant-like effect. Keywords: Xiaoyaosan, depression, chronic immobilization stress, tryptophan, TPH2, IDO1, traditional Chinese formula, traditional Chinese medicine
format	article
author	Jiao H Yan Z Ma Q Li X Jiang Y Liu Y Chen J
author_facet	Jiao H Yan Z Ma Q Li X Jiang Y Liu Y Chen J
author_sort	Jiao H
title	Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
title_short	Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
title_full	Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
title_fullStr	Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
title_full_unstemmed	Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
title_sort	influence of xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/88948e5a1ef94c8ab816aa1dac32727d
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Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus

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