Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings

Dan Huang,1 Kena Ma,1,2 Xinjie Cai,1,2 Xu Yang,3 Yinghui Hu,1 Pin Huang,1 Fushi Wang,1 Tao Jiang,1,2 Yining Wang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei- MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of...

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Autores principales:	Huang D, Ma K, Cai X, Yang X, Hu Y, Huang P, Wang F, Jiang T, Wang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	copper nanocomposite antibacterial angiogenic osteogenic Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8899cefb8cec451a90f61c082606fbd8
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spelling	oai:doaj.org-article:8899cefb8cec451a90f61c082606fbd82021-12-02T04:23:12ZEvaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings1178-2013https://doaj.org/article/8899cefb8cec451a90f61c082606fbd82017-10-01T00:00:00Zhttps://www.dovepress.com/evaluation-of-antibacterial-angiogenic-and-osteogenic-activities-of-gr-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Dan Huang,1 Kena Ma,1,2 Xinjie Cai,1,2 Xu Yang,3 Yinghui Hu,1 Pin Huang,1 Fushi Wang,1 Tao Jiang,1,2 Yining Wang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei- MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, China; 3Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA Abstract: Titanium (Ti) and its alloys have been widely used in clinics for years. However, their bio-inert surface challenges application in patients with compromised surgical conditions. Numerous studies were conducted to modify the surface topography and chemical composition of Ti substrates, for the purpose of obtaining antibacterial, angiogenic, and osteogenic activities. In this study, using green electrophoretic deposition method, we fabricated gap-bridging chitosan-gelatin (CSG) nanocomposite coatings incorporated with different amounts of copper (Cu; 0.01, 0.1, 1, and 10 mM for Cu I, II, III, and IV groups, respectively) on the Ti substrates. Physicochemical characterization of these coatings confirmed that Cu ions were successfully deposited into the coatings in a metallic status. After rehydration, the coatings swelled by 850% in weight. Mechanical tests verified the excellent tensile bond strength between Ti substrates and deposited coatings. All Cu-containing CSG coatings showed antibacterial property against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. The antibacterial property was positively correlated with the Cu concentration. In vitro cytocompatibility evaluation demonstrated that activities of bone marrow stromal cells were not impaired on Cu-doped coatings except for the Cu IV group. Moreover, enhanced angiogenic and osteogenic activities were observed on Cu II and Cu III groups. Overall, our results suggested that Cu-doped CSG nanocomposite coating is a promising candidate to functionalize Ti materials with antibacterial, angiogenic, and osteogenic properties. Keywords: copper, gap-bridging, antibacterial, angiogenic, osteogenic  Huang DMa KCai XYang XHu YHuang PWang FJiang TWang YDove Medical PressarticlecoppernanocompositeantibacterialangiogenicosteogenicMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7483-7500 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	copper nanocomposite antibacterial angiogenic osteogenic Medicine (General) R5-920
spellingShingle	copper nanocomposite antibacterial angiogenic osteogenic Medicine (General) R5-920 Huang D Ma K Cai X Yang X Hu Y Huang P Wang F Jiang T Wang Y Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
description	Dan Huang,1 Kena Ma,1,2 Xinjie Cai,1,2 Xu Yang,3 Yinghui Hu,1 Pin Huang,1 Fushi Wang,1 Tao Jiang,1,2 Yining Wang1,2 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei- MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 2Department of Prosthodontics, Hospital of Stomatology, Wuhan University, Wuhan, China; 3Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA Abstract: Titanium (Ti) and its alloys have been widely used in clinics for years. However, their bio-inert surface challenges application in patients with compromised surgical conditions. Numerous studies were conducted to modify the surface topography and chemical composition of Ti substrates, for the purpose of obtaining antibacterial, angiogenic, and osteogenic activities. In this study, using green electrophoretic deposition method, we fabricated gap-bridging chitosan-gelatin (CSG) nanocomposite coatings incorporated with different amounts of copper (Cu; 0.01, 0.1, 1, and 10 mM for Cu I, II, III, and IV groups, respectively) on the Ti substrates. Physicochemical characterization of these coatings confirmed that Cu ions were successfully deposited into the coatings in a metallic status. After rehydration, the coatings swelled by 850% in weight. Mechanical tests verified the excellent tensile bond strength between Ti substrates and deposited coatings. All Cu-containing CSG coatings showed antibacterial property against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus. The antibacterial property was positively correlated with the Cu concentration. In vitro cytocompatibility evaluation demonstrated that activities of bone marrow stromal cells were not impaired on Cu-doped coatings except for the Cu IV group. Moreover, enhanced angiogenic and osteogenic activities were observed on Cu II and Cu III groups. Overall, our results suggested that Cu-doped CSG nanocomposite coating is a promising candidate to functionalize Ti materials with antibacterial, angiogenic, and osteogenic properties. Keywords: copper, gap-bridging, antibacterial, angiogenic, osteogenic
format	article
author	Huang D Ma K Cai X Yang X Hu Y Huang P Wang F Jiang T Wang Y
author_facet	Huang D Ma K Cai X Yang X Hu Y Huang P Wang F Jiang T Wang Y
author_sort	Huang D
title	Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
title_short	Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
title_full	Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
title_fullStr	Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
title_full_unstemmed	Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
title_sort	evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8899cefb8cec451a90f61c082606fbd8
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Evaluation of antibacterial, angiogenic, and osteogenic activities of green synthesized gap-bridging copper-doped nanocomposite coatings

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