PCR based method for sex estimation from bone samples of unidentified South African fetal remains

Fragmentary bone in disaster victim identification is reliant on a robust and objective method. Current methods employing traditional phenotypic analysis for sex estimation of forensic fetal remains are limited and often not possible due to the lack of distinctive cranial and pelvic traits. Thus, a...

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Autores principales:	R. Thornton, E.F. Hutchinson, A.L. Edkins
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	G6PD SRY PCR Sex estimation Forensic Identification Genomic DNA isolation Perinatal remains Criminal law and procedure K5000-5582
Acceso en línea:	https://doaj.org/article/889f450093fd495b94ab1c6a2ae2088f
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spelling	oai:doaj.org-article:889f450093fd495b94ab1c6a2ae2088f2021-12-04T04:35:54ZPCR based method for sex estimation from bone samples of unidentified South African fetal remains2665-910710.1016/j.fsir.2021.100248https://doaj.org/article/889f450093fd495b94ab1c6a2ae2088f2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2665910721000797https://doaj.org/toc/2665-9107Fragmentary bone in disaster victim identification is reliant on a robust and objective method. Current methods employing traditional phenotypic analysis for sex estimation of forensic fetal remains are limited and often not possible due to the lack of distinctive cranial and pelvic traits. Thus, a molecular sex estimation method optimized for bone samples would enhance current techniques. Sternal rib samples were dissected from unclaimed perinatal decedents, presenting at varied stages of decomposition and unconfirmed circumstances of death, who were admitted to the Johannesburg Forensic Pathology Service (JFPS) and included in the Johannesburg Forensic Paediatric Collection (JFPC). A molecular protocol was designed and optimized using genomic DNA from SW480 cells and five individuals of unknown provenance and known sex (estimated during medicolegal autopsy) by targeting the X-linked G6PD and Y-linked SRY genes. The sex estimated at autopsy was confirmed using the optimized protocol. Nine (9) unidentified individuals were included and assigned sex based on the method described. The development of a sex typing protocol targeting the G6PD and SRY loci provides a new PCR method for sex estimation of human remains from 25 mg of bone fragment.R. ThorntonE.F. HutchinsonA.L. EdkinsElsevierarticleG6PDSRYPCR Sex estimationForensic IdentificationGenomic DNA isolationPerinatal remainsCriminal law and procedureK5000-5582ENForensic Science International: Reports, Vol 4, Iss , Pp 100248- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	G6PD SRY PCR Sex estimation Forensic Identification Genomic DNA isolation Perinatal remains Criminal law and procedure K5000-5582
spellingShingle	G6PD SRY PCR Sex estimation Forensic Identification Genomic DNA isolation Perinatal remains Criminal law and procedure K5000-5582 R. Thornton E.F. Hutchinson A.L. Edkins PCR based method for sex estimation from bone samples of unidentified South African fetal remains
description	Fragmentary bone in disaster victim identification is reliant on a robust and objective method. Current methods employing traditional phenotypic analysis for sex estimation of forensic fetal remains are limited and often not possible due to the lack of distinctive cranial and pelvic traits. Thus, a molecular sex estimation method optimized for bone samples would enhance current techniques. Sternal rib samples were dissected from unclaimed perinatal decedents, presenting at varied stages of decomposition and unconfirmed circumstances of death, who were admitted to the Johannesburg Forensic Pathology Service (JFPS) and included in the Johannesburg Forensic Paediatric Collection (JFPC). A molecular protocol was designed and optimized using genomic DNA from SW480 cells and five individuals of unknown provenance and known sex (estimated during medicolegal autopsy) by targeting the X-linked G6PD and Y-linked SRY genes. The sex estimated at autopsy was confirmed using the optimized protocol. Nine (9) unidentified individuals were included and assigned sex based on the method described. The development of a sex typing protocol targeting the G6PD and SRY loci provides a new PCR method for sex estimation of human remains from 25 mg of bone fragment.
format	article
author	R. Thornton E.F. Hutchinson A.L. Edkins
author_facet	R. Thornton E.F. Hutchinson A.L. Edkins
author_sort	R. Thornton
title	PCR based method for sex estimation from bone samples of unidentified South African fetal remains
title_short	PCR based method for sex estimation from bone samples of unidentified South African fetal remains
title_full	PCR based method for sex estimation from bone samples of unidentified South African fetal remains
title_fullStr	PCR based method for sex estimation from bone samples of unidentified South African fetal remains
title_full_unstemmed	PCR based method for sex estimation from bone samples of unidentified South African fetal remains
title_sort	pcr based method for sex estimation from bone samples of unidentified south african fetal remains
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/889f450093fd495b94ab1c6a2ae2088f
work_keys_str_mv	AT rthornton pcrbasedmethodforsexestimationfrombonesamplesofunidentifiedsouthafricanfetalremains AT efhutchinson pcrbasedmethodforsexestimationfrombonesamplesofunidentifiedsouthafricanfetalremains AT aledkins pcrbasedmethodforsexestimationfrombonesamplesofunidentifiedsouthafricanfetalremains
_version_	1718372924605333504

PCR based method for sex estimation from bone samples of unidentified South African fetal remains

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