Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)

<p><em>Helicoverpa armigera</em> Hubner (Lepidoptera: Noctuidae) is one of the most important pests on cotton. Yield losses due to this pest can reach up to 70%. Pest control using the NPV has been proven to be effective in the field. However NPV isolates from different geographica...

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Autores principales:	Farriza Diyasti, Teguh Santoso, R. Yayi Munara Kusumah
Formato:	article
Lenguaje:	EN ID
Publicado:	The Entomological Society of Indonesia 2017
Materias:	density, homology, LC, mortality, POB Zoology QL1-991
Acceso en línea:	https://doaj.org/article/88a31505ead54a66821ffb89863b67da
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spelling	oai:doaj.org-article:88a31505ead54a66821ffb89863b67da2021-12-02T04:12:44ZKarakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)1829-77222089-025710.5994/jei.13.3.117https://doaj.org/article/88a31505ead54a66821ffb89863b67da2017-06-01T00:00:00Zhttp://jurnal.pei-pusat.org/index.php/jei/article/view/116https://doaj.org/toc/1829-7722https://doaj.org/toc/2089-0257<p><em>Helicoverpa armigera</em> Hubner (Lepidoptera: Noctuidae) is one of the most important pests on cotton. Yield losses due to this pest can reach up to 70%. Pest control using the NPV has been proven to be effective in the field. However NPV isolates from different geographical locations could be showed variation in genetic and their biology. The purpose of this study was to compare morphology, biology, and molecular characteristics between three <em>Hear</em>NPV isolates from cotton field in South Sulawesi, Central Java, and East Java. Scanning electron microscope (SEM) was used to look at morphological characters, whereas biological characters were observed by dipping shelled of young corn cob on each isolate suspension with a density of 2.5 x 10<sup>3</sup>, 2.5 x 10<sup>4</sup>, 2.5 x 10<sup>5</sup>, 2.5 x 10<sup>6</sup>, 2.5 x 10<sup>7</sup> POBs /ml and control then fed to the first instar larvae of <em>H. armigera</em>. Probit analysis was used to determine LC and LT. The molecular assay used polymerase chain reaction<em> </em>(PCR) with lef-8 gene. The results showed that the three isolates had the shape and size of polyhedra which was not significantly different with the mean diameter were 0.86 μm. The result showed that in 7 days post infection, <em>Hear</em>NPV isolate from East Java was the most virulent isolates with the lowest LC<sub>50</sub>, which is 6.38 POBs/ml, but lowest LT<sub>50</sub> value in 2.5 x 10<sup>7 </sup>POBs/ml were showed both from East Java and South Sulawesi, which are 2.7 days post infection. Meanwhile, the DNA sequencing of the three isolates showed a high similarity (100% homology).</p>Farriza DiyastiTeguh SantosoR. Yayi Munara KusumahThe Entomological Society of Indonesiaarticledensity, homology, LC, mortality, POBZoologyQL1-991ENIDJurnal Entomologi Indonesia, Vol 13, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN ID
topic	density, homology, LC, mortality, POB Zoology QL1-991
spellingShingle	density, homology, LC, mortality, POB Zoology QL1-991 Farriza Diyasti Teguh Santoso R. Yayi Munara Kusumah Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
description	<p><em>Helicoverpa armigera</em> Hubner (Lepidoptera: Noctuidae) is one of the most important pests on cotton. Yield losses due to this pest can reach up to 70%. Pest control using the NPV has been proven to be effective in the field. However NPV isolates from different geographical locations could be showed variation in genetic and their biology. The purpose of this study was to compare morphology, biology, and molecular characteristics between three <em>Hear</em>NPV isolates from cotton field in South Sulawesi, Central Java, and East Java. Scanning electron microscope (SEM) was used to look at morphological characters, whereas biological characters were observed by dipping shelled of young corn cob on each isolate suspension with a density of 2.5 x 10<sup>3</sup>, 2.5 x 10<sup>4</sup>, 2.5 x 10<sup>5</sup>, 2.5 x 10<sup>6</sup>, 2.5 x 10<sup>7</sup> POBs /ml and control then fed to the first instar larvae of <em>H. armigera</em>. Probit analysis was used to determine LC and LT. The molecular assay used polymerase chain reaction<em> </em>(PCR) with lef-8 gene. The results showed that the three isolates had the shape and size of polyhedra which was not significantly different with the mean diameter were 0.86 μm. The result showed that in 7 days post infection, <em>Hear</em>NPV isolate from East Java was the most virulent isolates with the lowest LC<sub>50</sub>, which is 6.38 POBs/ml, but lowest LT<sub>50</sub> value in 2.5 x 10<sup>7 </sup>POBs/ml were showed both from East Java and South Sulawesi, which are 2.7 days post infection. Meanwhile, the DNA sequencing of the three isolates showed a high similarity (100% homology).</p>
format	article
author	Farriza Diyasti Teguh Santoso R. Yayi Munara Kusumah
author_facet	Farriza Diyasti Teguh Santoso R. Yayi Munara Kusumah
author_sort	Farriza Diyasti
title	Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
title_short	Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
title_full	Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
title_fullStr	Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
title_full_unstemmed	Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)
title_sort	karakter morfologi, biologi, dan molekuler tiga isolat helicoverpa armigera nucleopolyhedrovirus (hearnpv) terhadap penggerek buah kapas helicoverpa armigera hubner (lepidoptera: noctuidae)
publisher	The Entomological Society of Indonesia
publishDate	2017
url	https://doaj.org/article/88a31505ead54a66821ffb89863b67da
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Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae)

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