How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.

<h4>Background</h4>Mammalian sperms are activated in the oviduct. This process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (IVF).<h4>Methodology/principal findings</h4>Here we d...

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Autores principales:	Pei-Shiue Tsai, Núria Garcia-Gil, Theo van Haeften, Bart M Gadella
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88ab85e42f7742e4b2c73d65edb44c3f
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spelling	oai:doaj.org-article:88ab85e42f7742e4b2c73d65edb44c3f2021-12-02T20:20:41ZHow pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.1932-620310.1371/journal.pone.0011204https://doaj.org/article/88ab85e42f7742e4b2c73d65edb44c3f2010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20585455/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Mammalian sperms are activated in the oviduct. This process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (IVF).<h4>Methodology/principal findings</h4>Here we demonstrate that such treatments caused stable docking and priming of the acrosome membrane to the apical sperm head surface without the emergence of exocytotic membrane fusion. The interacting membranes could be isolated as bilamellar membrane structures after cell disruption. These membrane structures as well as whole capacitated sperm contained stable ternary trans-SNARE complexes that were composed of VAMP 3 and syntaxin 1B from the plasma membrane and SNAP 23 from the acrosomal membrane. This trans-SNARE complex was not observed in control sperm.<h4>Conclusions/significance</h4>We propose that this capacitation driven membrane docking and stability thereof is a preparative step prior to the multipoint membrane fusions characteristic for the acrosome reaction induced by sperm-zona binding. Thus, sperm can be considered a valuable model for studying exocytosis.Pei-Shiue TsaiNúria Garcia-GilTheo van HaeftenBart M GadellaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11204 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pei-Shiue Tsai Núria Garcia-Gil Theo van Haeften Bart M Gadella How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
description	<h4>Background</h4>Mammalian sperms are activated in the oviduct. This process, which involves extensive sperm surface remodelling, is required for fertilization and can be mimicked under in vitro fertilization conditions (IVF).<h4>Methodology/principal findings</h4>Here we demonstrate that such treatments caused stable docking and priming of the acrosome membrane to the apical sperm head surface without the emergence of exocytotic membrane fusion. The interacting membranes could be isolated as bilamellar membrane structures after cell disruption. These membrane structures as well as whole capacitated sperm contained stable ternary trans-SNARE complexes that were composed of VAMP 3 and syntaxin 1B from the plasma membrane and SNAP 23 from the acrosomal membrane. This trans-SNARE complex was not observed in control sperm.<h4>Conclusions/significance</h4>We propose that this capacitation driven membrane docking and stability thereof is a preparative step prior to the multipoint membrane fusions characteristic for the acrosome reaction induced by sperm-zona binding. Thus, sperm can be considered a valuable model for studying exocytosis.
format	article
author	Pei-Shiue Tsai Núria Garcia-Gil Theo van Haeften Bart M Gadella
author_facet	Pei-Shiue Tsai Núria Garcia-Gil Theo van Haeften Bart M Gadella
author_sort	Pei-Shiue Tsai
title	How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
title_short	How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
title_full	How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
title_fullStr	How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
title_full_unstemmed	How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
title_sort	how pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/88ab85e42f7742e4b2c73d65edb44c3f
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_version_	1718374175439060992

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.

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