MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae

The process of protein translocation into the endoplasmic reticulum (ER) is the initial and decisive step in the biosynthesis of all secretory proteins and many soluble organelle proteins. In this process, the Sec61 complex is the protein-conducting channel for transport. In this study, we identifie...

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Autores principales:	Yun-Yun Wei, Shuang Liang, Yun-Ran Zhang, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	sec61β development pathogenesis plant immunity er-phagy Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/88ac822df5844166899a2439876bb641
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spelling	oai:doaj.org-article:88ac822df5844166899a2439876bb6412021-11-17T14:21:59ZMoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae2150-55942150-560810.1080/21505594.2020.1848983https://doaj.org/article/88ac822df5844166899a2439876bb6412020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1848983https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The process of protein translocation into the endoplasmic reticulum (ER) is the initial and decisive step in the biosynthesis of all secretory proteins and many soluble organelle proteins. In this process, the Sec61 complex is the protein-conducting channel for transport. In this study, we identified and characterized the β subunit of the Sec61 complex in Magnaporthe oryzae (MoSec61β). Compared with the wild-type strain Guy11, the ΔMosec61β mutant exhibited highly branched mycelial morphology, reduced conidiation, high sensitivity to cell wall integrity stress, severely reduced virulence to rice and barley, and restricted biotrophic invasion. The turgor pressure of ΔMosec61β was notably reduced, which affected the function of appressoria. Moreover, ΔMosec61β was also sensitive to oxidative stress and exhibited a reduced ability to overcome plant immunity. Further examination demonstrated that MoSec61β affected the normal secretion of the apoplastic effectors Bas4 and Slp1. In addition, ΔMosec61β upregulated the level of ER-phagy. In conclusion, our results demonstrate the importance of the roles played by MoSec61β in the fungal development and pathogenesis of M. oryzae.Yun-Yun WeiShuang LiangYun-Ran ZhangJian-Ping LuFu-Cheng LinXiao-Hong LiuTaylor & Francis Grouparticlesec61βdevelopmentpathogenesisplant immunityer-phagyInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1685-1700 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	sec61β development pathogenesis plant immunity er-phagy Infectious and parasitic diseases RC109-216
spellingShingle	sec61β development pathogenesis plant immunity er-phagy Infectious and parasitic diseases RC109-216 Yun-Yun Wei Shuang Liang Yun-Ran Zhang Jian-Ping Lu Fu-Cheng Lin Xiao-Hong Liu MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
description	The process of protein translocation into the endoplasmic reticulum (ER) is the initial and decisive step in the biosynthesis of all secretory proteins and many soluble organelle proteins. In this process, the Sec61 complex is the protein-conducting channel for transport. In this study, we identified and characterized the β subunit of the Sec61 complex in Magnaporthe oryzae (MoSec61β). Compared with the wild-type strain Guy11, the ΔMosec61β mutant exhibited highly branched mycelial morphology, reduced conidiation, high sensitivity to cell wall integrity stress, severely reduced virulence to rice and barley, and restricted biotrophic invasion. The turgor pressure of ΔMosec61β was notably reduced, which affected the function of appressoria. Moreover, ΔMosec61β was also sensitive to oxidative stress and exhibited a reduced ability to overcome plant immunity. Further examination demonstrated that MoSec61β affected the normal secretion of the apoplastic effectors Bas4 and Slp1. In addition, ΔMosec61β upregulated the level of ER-phagy. In conclusion, our results demonstrate the importance of the roles played by MoSec61β in the fungal development and pathogenesis of M. oryzae.
format	article
author	Yun-Yun Wei Shuang Liang Yun-Ran Zhang Jian-Ping Lu Fu-Cheng Lin Xiao-Hong Liu
author_facet	Yun-Yun Wei Shuang Liang Yun-Ran Zhang Jian-Ping Lu Fu-Cheng Lin Xiao-Hong Liu
author_sort	Yun-Yun Wei
title	MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
title_short	MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
title_full	MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
title_fullStr	MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
title_full_unstemmed	MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
title_sort	mosec61β, the beta subunit of sec61, is involved in fungal development and pathogenicity, plant immunity, and er-phagy in magnaporthe oryzae
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/88ac822df5844166899a2439876bb641
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MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae

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