The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells

Abstract Graphene quantum dots (GQDs) are a promising next generation nanomaterial with manifold biomedical applications. For real world applications, comprehensive studies on their influence on the functionality of primary human cells are mandatory. Here, we report the effects of GQDs on the transc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Stefan Fasbender, Lisa Zimmermann, Ron-Patrick Cadeddu, Martina Luysberg, Bastian Moll, Christoph Janiak, Thomas Heinzel, Rainer Haas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88ad72d70dcb4bbf89839d764d7aff1e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:88ad72d70dcb4bbf89839d764d7aff1e
record_format	dspace
spelling	oai:doaj.org-article:88ad72d70dcb4bbf89839d764d7aff1e2021-12-02T16:08:05ZThe Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells10.1038/s41598-019-48567-62045-2322https://doaj.org/article/88ad72d70dcb4bbf89839d764d7aff1e2019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48567-6https://doaj.org/toc/2045-2322Abstract Graphene quantum dots (GQDs) are a promising next generation nanomaterial with manifold biomedical applications. For real world applications, comprehensive studies on their influence on the functionality of primary human cells are mandatory. Here, we report the effects of GQDs on the transcriptome of CD34+ hematopoietic stem cells after an incubation time of 36 hours. Of the 20 800 recorded gene expressions, only one, namely the selenoprotein W, 1, is changed by the GQDs in direct comparison to CD34+ hematopoietic stem cells cultivated without GQDs. Only a meta analysis reveals that the expression of 1171 genes is weakly affected, taking into account the more prominent changes just by the cell culture. Eight corresponding, weakly affected signaling pathways are identified, which include, but are not limited to, the triggering of apoptosis. These results suggest that GQDs with sizes in the range of a few nanometers hardly influence the CD34+ cells on the transcriptome level after 36 h of incubation, thereby demonstrating their high usability for in vivo studies, such as fluorescence labeling or delivery protocols, without strong effects on the functional status of the cells.Stefan FasbenderLisa ZimmermannRon-Patrick CadedduMartina LuysbergBastian MollChristoph JaniakThomas HeinzelRainer HaasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan Fasbender Lisa Zimmermann Ron-Patrick Cadeddu Martina Luysberg Bastian Moll Christoph Janiak Thomas Heinzel Rainer Haas The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
description	Abstract Graphene quantum dots (GQDs) are a promising next generation nanomaterial with manifold biomedical applications. For real world applications, comprehensive studies on their influence on the functionality of primary human cells are mandatory. Here, we report the effects of GQDs on the transcriptome of CD34+ hematopoietic stem cells after an incubation time of 36 hours. Of the 20 800 recorded gene expressions, only one, namely the selenoprotein W, 1, is changed by the GQDs in direct comparison to CD34+ hematopoietic stem cells cultivated without GQDs. Only a meta analysis reveals that the expression of 1171 genes is weakly affected, taking into account the more prominent changes just by the cell culture. Eight corresponding, weakly affected signaling pathways are identified, which include, but are not limited to, the triggering of apoptosis. These results suggest that GQDs with sizes in the range of a few nanometers hardly influence the CD34+ cells on the transcriptome level after 36 h of incubation, thereby demonstrating their high usability for in vivo studies, such as fluorescence labeling or delivery protocols, without strong effects on the functional status of the cells.
format	article
author	Stefan Fasbender Lisa Zimmermann Ron-Patrick Cadeddu Martina Luysberg Bastian Moll Christoph Janiak Thomas Heinzel Rainer Haas
author_facet	Stefan Fasbender Lisa Zimmermann Ron-Patrick Cadeddu Martina Luysberg Bastian Moll Christoph Janiak Thomas Heinzel Rainer Haas
author_sort	Stefan Fasbender
title	The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
title_short	The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
title_full	The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
title_fullStr	The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
title_full_unstemmed	The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells
title_sort	low toxicity of graphene quantum dots is reflected by marginal gene expression changes of primary human hematopoietic stem cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/88ad72d70dcb4bbf89839d764d7aff1e
work_keys_str_mv	AT stefanfasbender thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT lisazimmermann thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT ronpatrickcadeddu thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT martinaluysberg thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT bastianmoll thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT christophjaniak thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT thomasheinzel thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT rainerhaas thelowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT stefanfasbender lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT lisazimmermann lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT ronpatrickcadeddu lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT martinaluysberg lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT bastianmoll lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT christophjaniak lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT thomasheinzel lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells AT rainerhaas lowtoxicityofgraphenequantumdotsisreflectedbymarginalgeneexpressionchangesofprimaryhumanhematopoieticstemcells
_version_	1718384608582565888

The Low Toxicity of Graphene Quantum Dots is Reflected by Marginal Gene Expression Changes of Primary Human Hematopoietic Stem Cells

Ejemplares similares