A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge

Currently, a fatal disease of hepatitis-hydropericardium syndrome (HHS) caused by serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and resulted in tremendous economic losses to the poultry industry. Various vaccines against FAdV-4 were developed to control the disease; however, few live-atte...

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Autores principales:	Yaru Mu, Quan Xie, Weikang Wang, Hao Lu, Mingjun Lian, Wei Gao, Tuofan Li, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	FAdV-4 fiber-1 CRISPR/Cas9 recombinant virus attenuation protection Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/88af31a0542f4d5b8de3b3365a0d63f8
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spelling	oai:doaj.org-article:88af31a0542f4d5b8de3b3365a0d63f82021-11-22T07:27:20ZA Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge2297-176910.3389/fvets.2021.759418https://doaj.org/article/88af31a0542f4d5b8de3b3365a0d63f82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.759418/fullhttps://doaj.org/toc/2297-1769Currently, a fatal disease of hepatitis-hydropericardium syndrome (HHS) caused by serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and resulted in tremendous economic losses to the poultry industry. Various vaccines against FAdV-4 were developed to control the disease; however, few live-attenuated vaccines were available. In this study, we targeted the N-terminal of fiber-1 and rescued a recombinant virus FAdV4-RFP_F1 expressing the fusion protein of RFP and Fiber-1 based on the CRISPR/Cas9 technique. In vitro studies showed that FAdV4-RFP_F1 replicated slower than the wild type FAdV-4, but the peak viral titer of FAdV4-RFP_F1 could still reach 107.0 TCID50/ml with high stability in LMH cells. Animal studies found that FAdV4-RFP_F1 not only was highly attenuated to the 2-week-old SPF chickens, but could also provide efficient protection against lethal challenge of FAdV-4. All these demonstrate that the recombinant virus FAdV4-RFP_F1 could be as an efficient live-attenuated vaccine candidate for FAdV-4, and the N-terminal of fiber-1 could be as a potential insertion site for expressing foreign genes to develop FAdV-4-based vaccine.Yaru MuYaru MuYaru MuYaru MuQuan XieQuan XieQuan XieQuan XieWeikang WangWeikang WangWeikang WangWeikang WangHao LuHao LuHao LuHao LuMingjun LianMingjun LianMingjun LianMingjun LianWei GaoWei GaoWei GaoWei GaoTuofan LiTuofan LiTuofan LiTuofan LiZhimin WanZhimin WanZhimin WanZhimin WanHongxia ShaoHongxia ShaoHongxia ShaoHongxia ShaoAijian QinAijian QinAijian QinAijian QinJianqiang YeJianqiang YeJianqiang YeJianqiang YeFrontiers Media S.A.articleFAdV-4fiber-1CRISPR/Cas9recombinant virusattenuationprotectionVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	FAdV-4 fiber-1 CRISPR/Cas9 recombinant virus attenuation protection Veterinary medicine SF600-1100
spellingShingle	FAdV-4 fiber-1 CRISPR/Cas9 recombinant virus attenuation protection Veterinary medicine SF600-1100 Yaru Mu Yaru Mu Yaru Mu Yaru Mu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Hao Lu Hao Lu Hao Lu Hao Lu Mingjun Lian Mingjun Lian Mingjun Lian Mingjun Lian Wei Gao Wei Gao Wei Gao Wei Gao Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
description	Currently, a fatal disease of hepatitis-hydropericardium syndrome (HHS) caused by serotype 4 fowl adenovirus (FAdV-4) has spread worldwide and resulted in tremendous economic losses to the poultry industry. Various vaccines against FAdV-4 were developed to control the disease; however, few live-attenuated vaccines were available. In this study, we targeted the N-terminal of fiber-1 and rescued a recombinant virus FAdV4-RFP_F1 expressing the fusion protein of RFP and Fiber-1 based on the CRISPR/Cas9 technique. In vitro studies showed that FAdV4-RFP_F1 replicated slower than the wild type FAdV-4, but the peak viral titer of FAdV4-RFP_F1 could still reach 107.0 TCID50/ml with high stability in LMH cells. Animal studies found that FAdV4-RFP_F1 not only was highly attenuated to the 2-week-old SPF chickens, but could also provide efficient protection against lethal challenge of FAdV-4. All these demonstrate that the recombinant virus FAdV4-RFP_F1 could be as an efficient live-attenuated vaccine candidate for FAdV-4, and the N-terminal of fiber-1 could be as a potential insertion site for expressing foreign genes to develop FAdV-4-based vaccine.
format	article
author	Yaru Mu Yaru Mu Yaru Mu Yaru Mu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Hao Lu Hao Lu Hao Lu Hao Lu Mingjun Lian Mingjun Lian Mingjun Lian Mingjun Lian Wei Gao Wei Gao Wei Gao Wei Gao Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye
author_facet	Yaru Mu Yaru Mu Yaru Mu Yaru Mu Quan Xie Quan Xie Quan Xie Quan Xie Weikang Wang Weikang Wang Weikang Wang Weikang Wang Hao Lu Hao Lu Hao Lu Hao Lu Mingjun Lian Mingjun Lian Mingjun Lian Mingjun Lian Wei Gao Wei Gao Wei Gao Wei Gao Tuofan Li Tuofan Li Tuofan Li Tuofan Li Zhimin Wan Zhimin Wan Zhimin Wan Zhimin Wan Hongxia Shao Hongxia Shao Hongxia Shao Hongxia Shao Aijian Qin Aijian Qin Aijian Qin Aijian Qin Jianqiang Ye Jianqiang Ye Jianqiang Ye Jianqiang Ye
author_sort	Yaru Mu
title	A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
title_short	A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
title_full	A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
title_fullStr	A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
title_full_unstemmed	A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge
title_sort	novel fiber-1-edited and highly attenuated recombinant serotype 4 fowl adenovirus confers efficient protection against lethal challenge
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/88af31a0542f4d5b8de3b3365a0d63f8
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A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge

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