A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus

OCD20015-V009 is an herbal mix of water-extracted Ginseng Radix, Poria (Hoelen), Rehmanniae Radix, Adenophorae Radix, Platycodi Radix, Crataegii Fructus, and Astragali Radix. In this study, its in vitro and in vivo antiviral activity and mechanisms against the influenza A virus were evaluated using...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Eun-Bin Kwon, You-Chang Oh, Youn-Hwan Hwang, Wei Li, Seok-Man Park, Ryong Kong, Young Soo Kim, Jang-Gi Choi
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	OCD20015-V009 influenza A virus antiviral activity interferon herbal complex medicine Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/88b3faabc9a04343bf982cd7977dcf38
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:88b3faabc9a04343bf982cd7977dcf38
record_format	dspace
spelling	oai:doaj.org-article:88b3faabc9a04343bf982cd7977dcf382021-11-30T17:49:08ZA Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus1663-981210.3389/fphar.2021.764297https://doaj.org/article/88b3faabc9a04343bf982cd7977dcf382021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.764297/fullhttps://doaj.org/toc/1663-9812OCD20015-V009 is an herbal mix of water-extracted Ginseng Radix, Poria (Hoelen), Rehmanniae Radix, Adenophorae Radix, Platycodi Radix, Crataegii Fructus, and Astragali Radix. In this study, its in vitro and in vivo antiviral activity and mechanisms against the influenza A virus were evaluated using a GFP-tagged influenza A virus (A/PR/8/34-GFP) to infect murine macrophages. We found that OCD20015-V009 pre-treatment substantially reduced A/PR/8/34-GFP replication. Also, OCD20015-V009 pre-treatment increased the phosphorylation of type-I IFN-related proteins TBK-1 and STAT1 and the secretion of pro-inflammatory cytokines TNF-α and IL-6 by murine macrophages. Moreover, OCD20015-V009 prophylactic administration increased IFN-stimulated genes-related 15, 20, and 56 and IFN-β mRNA in vitro. Thus, OCD20015-V009 likely modulates murine innate immune response via macrophages. This finding is potentially useful for developing prophylactics or therapeutics against the influenza A virus. Furthermore, pre-treatment with OCD20015-V009 decreased the mortality of the mice exposed to A/PR/8/34-GFP by 20% compared to that in the untreated animals. Thus, OCD20015-V009 stimulates the antiviral response in murine macrophages and mice to viral infections. Additionally, we identified chlorogenic acid and ginsenoside Rd as the antiviral components in OCD20015-V009. Further investigations are needed to elucidate the protective effects of active components of OCD20015-V009 against influenza A viruses.Eun-Bin KwonYou-Chang OhYoun-Hwan HwangWei LiSeok-Man ParkRyong KongYoung Soo KimJang-Gi ChoiFrontiers Media S.A.articleOCD20015-V009influenza A virusantiviral activityinterferonherbal complex medicineTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	OCD20015-V009 influenza A virus antiviral activity interferon herbal complex medicine Therapeutics. Pharmacology RM1-950
spellingShingle	OCD20015-V009 influenza A virus antiviral activity interferon herbal complex medicine Therapeutics. Pharmacology RM1-950 Eun-Bin Kwon You-Chang Oh Youn-Hwan Hwang Wei Li Seok-Man Park Ryong Kong Young Soo Kim Jang-Gi Choi A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
description	OCD20015-V009 is an herbal mix of water-extracted Ginseng Radix, Poria (Hoelen), Rehmanniae Radix, Adenophorae Radix, Platycodi Radix, Crataegii Fructus, and Astragali Radix. In this study, its in vitro and in vivo antiviral activity and mechanisms against the influenza A virus were evaluated using a GFP-tagged influenza A virus (A/PR/8/34-GFP) to infect murine macrophages. We found that OCD20015-V009 pre-treatment substantially reduced A/PR/8/34-GFP replication. Also, OCD20015-V009 pre-treatment increased the phosphorylation of type-I IFN-related proteins TBK-1 and STAT1 and the secretion of pro-inflammatory cytokines TNF-α and IL-6 by murine macrophages. Moreover, OCD20015-V009 prophylactic administration increased IFN-stimulated genes-related 15, 20, and 56 and IFN-β mRNA in vitro. Thus, OCD20015-V009 likely modulates murine innate immune response via macrophages. This finding is potentially useful for developing prophylactics or therapeutics against the influenza A virus. Furthermore, pre-treatment with OCD20015-V009 decreased the mortality of the mice exposed to A/PR/8/34-GFP by 20% compared to that in the untreated animals. Thus, OCD20015-V009 stimulates the antiviral response in murine macrophages and mice to viral infections. Additionally, we identified chlorogenic acid and ginsenoside Rd as the antiviral components in OCD20015-V009. Further investigations are needed to elucidate the protective effects of active components of OCD20015-V009 against influenza A viruses.
format	article
author	Eun-Bin Kwon You-Chang Oh Youn-Hwan Hwang Wei Li Seok-Man Park Ryong Kong Young Soo Kim Jang-Gi Choi
author_facet	Eun-Bin Kwon You-Chang Oh Youn-Hwan Hwang Wei Li Seok-Man Park Ryong Kong Young Soo Kim Jang-Gi Choi
author_sort	Eun-Bin Kwon
title	A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
title_short	A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
title_full	A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
title_fullStr	A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
title_full_unstemmed	A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus
title_sort	herbal mixture formula of ocd20015-v009 prophylactic administration to enhance interferon-mediated antiviral activity against influenza a virus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/88b3faabc9a04343bf982cd7977dcf38
work_keys_str_mv	AT eunbinkwon aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT youchangoh aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT younhwanhwang aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT weili aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT seokmanpark aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT ryongkong aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT youngsookim aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT janggichoi aherbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT eunbinkwon herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT youchangoh herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT younhwanhwang herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT weili herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT seokmanpark herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT ryongkong herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT youngsookim herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus AT janggichoi herbalmixtureformulaofocd20015v009prophylacticadministrationtoenhanceinterferonmediatedantiviralactivityagainstinfluenzaavirus
_version_	1718406391267328000

A Herbal Mixture Formula of OCD20015-V009 Prophylactic Administration to Enhance Interferon-Mediated Antiviral Activity Against Influenza A Virus

Ejemplares similares