Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar

Abstract Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with adv...

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Autores principales:	Maria Gay C. Carrillo, Federico Martin, Mukund Variar, J. C. Bhatt, Alvaro L. Perez-Quintero, Hei Leung, Jan E. Leach, Casiana M. Vera Cruz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88bb277b5c73475891ece9de2585c61d
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spelling	oai:doaj.org-article:88bb277b5c73475891ece9de2585c61d2021-11-08T10:48:55ZAccumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar10.1038/s41598-021-00759-92045-2322https://doaj.org/article/88bb277b5c73475891ece9de2585c61d2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00759-9https://doaj.org/toc/2045-2322Abstract Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with advanced backcross populations, can accelerate the production of more resistant varieties. A candidate gene (CG) approach was used to accumulate different disease QTL from Moroberekan, a blast-resistant rice variety, into Vandana, a drought-tolerant variety. The advanced backcross progeny were evaluated for resistance to blast and tolerance to drought at five sites in India and the Philippines. Gene-based markers were designed to determine introgression of Moroberekan alleles for 11 CGs into the progeny. Six CGs, coding for chitinase, HSP90, oxalate oxidase, germin-like proteins, peroxidase and thaumatin-like protein, and 21 SSR markers were significantly associated with resistance to blast across screening sites. Multiple lines with different combinations, classes and numbers of CGs were associated with significant levels of race non-specific resistance to rice blast and sheath blight. Overall, the level of resistance effective in multiple locations was proportional to the number of CG alleles accumulated in advanced breeding lines. These disease resistant lines maintained tolerance to drought stress at the reproductive stage under blast disease pressure.Maria Gay C. CarrilloFederico MartinMukund VariarJ. C. BhattAlvaro L. Perez-QuinteroHei LeungJan E. LeachCasiana M. Vera CruzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Gay C. Carrillo Federico Martin Mukund Variar J. C. Bhatt Alvaro L. Perez-Quintero Hei Leung Jan E. Leach Casiana M. Vera Cruz Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
description	Abstract Biotic stresses, including diseases, severely affect rice production, compromising producers’ ability to meet increasing global consumption. Understanding quantitative responses for resistance to diverse pathogens can guide development of reliable molecular markers, which, combined with advanced backcross populations, can accelerate the production of more resistant varieties. A candidate gene (CG) approach was used to accumulate different disease QTL from Moroberekan, a blast-resistant rice variety, into Vandana, a drought-tolerant variety. The advanced backcross progeny were evaluated for resistance to blast and tolerance to drought at five sites in India and the Philippines. Gene-based markers were designed to determine introgression of Moroberekan alleles for 11 CGs into the progeny. Six CGs, coding for chitinase, HSP90, oxalate oxidase, germin-like proteins, peroxidase and thaumatin-like protein, and 21 SSR markers were significantly associated with resistance to blast across screening sites. Multiple lines with different combinations, classes and numbers of CGs were associated with significant levels of race non-specific resistance to rice blast and sheath blight. Overall, the level of resistance effective in multiple locations was proportional to the number of CG alleles accumulated in advanced breeding lines. These disease resistant lines maintained tolerance to drought stress at the reproductive stage under blast disease pressure.
format	article
author	Maria Gay C. Carrillo Federico Martin Mukund Variar J. C. Bhatt Alvaro L. Perez-Quintero Hei Leung Jan E. Leach Casiana M. Vera Cruz
author_facet	Maria Gay C. Carrillo Federico Martin Mukund Variar J. C. Bhatt Alvaro L. Perez-Quintero Hei Leung Jan E. Leach Casiana M. Vera Cruz
author_sort	Maria Gay C. Carrillo
title	Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_short	Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_full	Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_fullStr	Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_full_unstemmed	Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
title_sort	accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/88bb277b5c73475891ece9de2585c61d
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Accumulating candidate genes for broad-spectrum resistance to rice blast in a drought-tolerant rice cultivar

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