Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin

Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin

Seyin Zou,1,3,† Nuo Cao,1,† Du Cheng,2 Rongqin Zheng,4 Jin Wang,4 Kangshun Zhu,4 Xintao Shuai1,2 1Center of Biomedical Engineering, Zhongshan School of Medicine, 2PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, 3Department of Laboratory...

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Autores principales: Zou S, Cao N, Cheng D, Zheng R, Wang J, Zhu K, Shuai X
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/88ca8cf3cedb470b8861e16672125350
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spelling oai:doaj.org-article:88ca8cf3cedb470b8861e166721253502021-12-02T02:43:34ZEnhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin1176-91141178-2013https://doaj.org/article/88ca8cf3cedb470b8861e166721253502012-07-01T00:00:00Zhttp://www.dovepress.com/enhanced-apoptosis-of-ovarian-cancer-cells-via-nanocarrier-mediated-co-a10445https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Seyin Zou,1,3,† Nuo Cao,1,† Du Cheng,2 Rongqin Zheng,4 Jin Wang,4 Kangshun Zhu,4 Xintao Shuai1,2 1Center of Biomedical Engineering, Zhongshan School of Medicine, 2PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, 3Department of Laboratory Medicine,The Third Affiliated Hospital, Guangzhou Medical University, 4The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China †These authors contributed equally to this workAbstract: A folate conjugated ternary copolymer, FA–PEG–PEI–PCL, of poly(ethylene glycol) (PEG), poly(ethylene imine) (PEI), and poly(ε-caprolactone) (PCL) was synthesized. The copolymer self-assembled into cationic micelles capable of co-delivering siRNA and the anticancer drug doxorubicin (DOX). This dual functional nanocarrier demonstrated low cytotoxicity and high performance in drug/siRNA delivery. Upon the codelivery of siRNA, targeting the Bcl-2 gene, and DOX, using the folate-targeted nanocarrier, DOX-induced apoptosis in the skov-3 cells overexpressing folate receptor was significantly enhanced through a mechanism of downregulating the antiapoptotic protein Bcl-2, while simultaneously upregulating the proapoptotic protein Bax. This work suggested that the combination of Bcl-2 siRNA and DOX therapies is feasible, based on our dual functional nanocarrier, which set up a good basis for a future in vivo test.Keywords: codelivery, gene silencing, chemotherapy, apoptosis, tumor targetingZou SCao NCheng DZheng RWang JZhu KShuai XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3823-3835 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Zou S
Cao N
Cheng D
Zheng R
Wang J
Zhu K
Shuai X
Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
description Seyin Zou,1,3,† Nuo Cao,1,† Du Cheng,2 Rongqin Zheng,4 Jin Wang,4 Kangshun Zhu,4 Xintao Shuai1,2 1Center of Biomedical Engineering, Zhongshan School of Medicine, 2PCFM Lab of Ministry of Education, School of Chemistry and Chemical Engineering, 3Department of Laboratory Medicine,The Third Affiliated Hospital, Guangzhou Medical University, 4The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China †These authors contributed equally to this workAbstract: A folate conjugated ternary copolymer, FA–PEG–PEI–PCL, of poly(ethylene glycol) (PEG), poly(ethylene imine) (PEI), and poly(ε-caprolactone) (PCL) was synthesized. The copolymer self-assembled into cationic micelles capable of co-delivering siRNA and the anticancer drug doxorubicin (DOX). This dual functional nanocarrier demonstrated low cytotoxicity and high performance in drug/siRNA delivery. Upon the codelivery of siRNA, targeting the Bcl-2 gene, and DOX, using the folate-targeted nanocarrier, DOX-induced apoptosis in the skov-3 cells overexpressing folate receptor was significantly enhanced through a mechanism of downregulating the antiapoptotic protein Bcl-2, while simultaneously upregulating the proapoptotic protein Bax. This work suggested that the combination of Bcl-2 siRNA and DOX therapies is feasible, based on our dual functional nanocarrier, which set up a good basis for a future in vivo test.Keywords: codelivery, gene silencing, chemotherapy, apoptosis, tumor targeting
format article
author Zou S
Cao N
Cheng D
Zheng R
Wang J
Zhu K
Shuai X
author_facet Zou S
Cao N
Cheng D
Zheng R
Wang J
Zhu K
Shuai X
author_sort Zou S
title Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
title_short Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
title_full Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
title_fullStr Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
title_full_unstemmed Enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of siRNA and doxorubicin
title_sort enhanced apoptosis of ovarian cancer cells via nanocarrier-mediated codelivery of sirna and doxorubicin
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/88ca8cf3cedb470b8861e16672125350
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AT caon enhancedapoptosisofovariancancercellsviananocarriermediatedcodeliveryofsirnaanddoxorubicin
AT chengd enhancedapoptosisofovariancancercellsviananocarriermediatedcodeliveryofsirnaanddoxorubicin
AT zhengr enhancedapoptosisofovariancancercellsviananocarriermediatedcodeliveryofsirnaanddoxorubicin
AT wangj enhancedapoptosisofovariancancercellsviananocarriermediatedcodeliveryofsirnaanddoxorubicin
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