In TFIIH, XPD helicase is exclusively devoted to DNA repair.

The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations in human XPD are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. We performed a comparativ...

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Autores principales:	Jochen Kuper, Cathy Braun, Agnes Elias, Gudrun Michels, Florian Sauer, Dominik R Schmitt, Arnaud Poterszman, Jean-Marc Egly, Caroline Kisker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/88cc70ed5c364099a3152604a61d9dd6
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spelling	oai:doaj.org-article:88cc70ed5c364099a3152604a61d9dd62021-11-25T05:32:56ZIn TFIIH, XPD helicase is exclusively devoted to DNA repair.1544-91731545-788510.1371/journal.pbio.1001954https://doaj.org/article/88cc70ed5c364099a3152604a61d9dd62014-09-01T00:00:00Zhttps://doi.org/10.1371/journal.pbio.1001954https://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations in human XPD are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. We performed a comparative analysis of XPD from Homo sapiens and Chaetomium thermophilum (a closely related thermostable fungal orthologue) to decipher the different molecular prerequisites necessary for either transcription or DNA repair. In vitro and in vivo assays demonstrate that mutations in the 4Fe4S cluster domain of XPD abrogate the NER function of TFIIH and do not affect its transcriptional activity. We show that the p44-dependent activation of XPD is promoted by the stimulation of its ATPase activity. Furthermore, we clearly demonstrate that XPD requires DNA binding, ATPase, and helicase activity to function in NER. In contrast, these enzymatic properties are dispensable for transcription initiation. XPD helicase is thus exclusively devoted to NER and merely acts as a structural scaffold to maintain TFIIH integrity during transcription.Jochen KuperCathy BraunAgnes EliasGudrun MichelsFlorian SauerDominik R SchmittArnaud PoterszmanJean-Marc EglyCaroline KiskerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 9, p e1001954 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jochen Kuper Cathy Braun Agnes Elias Gudrun Michels Florian Sauer Dominik R Schmitt Arnaud Poterszman Jean-Marc Egly Caroline Kisker In TFIIH, XPD helicase is exclusively devoted to DNA repair.
description	The eukaryotic XPD helicase is an essential subunit of TFIIH involved in both transcription and nucleotide excision repair (NER). Mutations in human XPD are associated with several inherited diseases such as xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. We performed a comparative analysis of XPD from Homo sapiens and Chaetomium thermophilum (a closely related thermostable fungal orthologue) to decipher the different molecular prerequisites necessary for either transcription or DNA repair. In vitro and in vivo assays demonstrate that mutations in the 4Fe4S cluster domain of XPD abrogate the NER function of TFIIH and do not affect its transcriptional activity. We show that the p44-dependent activation of XPD is promoted by the stimulation of its ATPase activity. Furthermore, we clearly demonstrate that XPD requires DNA binding, ATPase, and helicase activity to function in NER. In contrast, these enzymatic properties are dispensable for transcription initiation. XPD helicase is thus exclusively devoted to NER and merely acts as a structural scaffold to maintain TFIIH integrity during transcription.
format	article
author	Jochen Kuper Cathy Braun Agnes Elias Gudrun Michels Florian Sauer Dominik R Schmitt Arnaud Poterszman Jean-Marc Egly Caroline Kisker
author_facet	Jochen Kuper Cathy Braun Agnes Elias Gudrun Michels Florian Sauer Dominik R Schmitt Arnaud Poterszman Jean-Marc Egly Caroline Kisker
author_sort	Jochen Kuper
title	In TFIIH, XPD helicase is exclusively devoted to DNA repair.
title_short	In TFIIH, XPD helicase is exclusively devoted to DNA repair.
title_full	In TFIIH, XPD helicase is exclusively devoted to DNA repair.
title_fullStr	In TFIIH, XPD helicase is exclusively devoted to DNA repair.
title_full_unstemmed	In TFIIH, XPD helicase is exclusively devoted to DNA repair.
title_sort	in tfiih, xpd helicase is exclusively devoted to dna repair.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/88cc70ed5c364099a3152604a61d9dd6
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In TFIIH, XPD helicase is exclusively devoted to DNA repair.

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