Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry

Abstract Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of co...

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Autores principales:	Karen Reiser, Patrick Stoller, André Knoesen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88ce07aad942428f80f23068808cb2c9
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spelling	oai:doaj.org-article:88ce07aad942428f80f23068808cb2c92021-12-02T16:06:30ZThree-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry10.1038/s41598-017-02326-72045-2322https://doaj.org/article/88ce07aad942428f80f23068808cb2c92017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02326-7https://doaj.org/toc/2045-2322Abstract Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of collagen fibrils could be determined by modulating the polarization angle of the laser during scanning. We have now extended this method so that out-of-plane orientation angles can be determined at the same time, allowing visualization of the 3-dimensional structure of collagenous tissues. This approach offers advantages compared with other methods for determining out-of-plane orientation. First, the orientation angles are directly calculated from the polarimetry data obtained in a single scan, while other reported methods require data from multiple scans, use of iterative optimization methods, application of fitting algorithms, or extensive post-optical processing. Second, our method does not require highly specialized instrumentation, and thus can be adapted for use in almost any nonlinear optical microscopy setup. It is suitable for both basic and clinical applications. We present three-dimensional images of structurally complex collagenous tissues that illustrate the power of such 3-dimensional analyses to reveal the architecture of biological structures.Karen ReiserPatrick StollerAndré KnoesenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karen Reiser Patrick Stoller André Knoesen Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
description	Abstract Collagen is a biological macromolecule capable of second harmonic generation, allowing label-free detection in tissues; in addition, molecular orientation can be determined from the polarization dependence of the second harmonic signal. Previously we reported that in-plane orientation of collagen fibrils could be determined by modulating the polarization angle of the laser during scanning. We have now extended this method so that out-of-plane orientation angles can be determined at the same time, allowing visualization of the 3-dimensional structure of collagenous tissues. This approach offers advantages compared with other methods for determining out-of-plane orientation. First, the orientation angles are directly calculated from the polarimetry data obtained in a single scan, while other reported methods require data from multiple scans, use of iterative optimization methods, application of fitting algorithms, or extensive post-optical processing. Second, our method does not require highly specialized instrumentation, and thus can be adapted for use in almost any nonlinear optical microscopy setup. It is suitable for both basic and clinical applications. We present three-dimensional images of structurally complex collagenous tissues that illustrate the power of such 3-dimensional analyses to reveal the architecture of biological structures.
format	article
author	Karen Reiser Patrick Stoller André Knoesen
author_facet	Karen Reiser Patrick Stoller André Knoesen
author_sort	Karen Reiser
title	Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
title_short	Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
title_full	Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
title_fullStr	Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
title_full_unstemmed	Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry
title_sort	three-dimensional geometry of collagenous tissues by second harmonic polarimetry
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/88ce07aad942428f80f23068808cb2c9
work_keys_str_mv	AT karenreiser threedimensionalgeometryofcollagenoustissuesbysecondharmonicpolarimetry AT patrickstoller threedimensionalgeometryofcollagenoustissuesbysecondharmonicpolarimetry AT andreknoesen threedimensionalgeometryofcollagenoustissuesbysecondharmonicpolarimetry
_version_	1718384974073167872

Three-Dimensional Geometry of Collagenous Tissues by Second Harmonic Polarimetry

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