TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER

To identify new effective targets for immunotherapy of breast cancer, based on cancer-testis antigens (CTA), the associations were studied between CTA gene expression, clinical and pathological characteristics of breast cancer. Therefore, the aim of the study was to perform screening of CTAs specifi...

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Autores principales:	D. I. Vodolazhskiy, D. S. Kutilin, Kh. A. Mogushkova, O. I. Kit
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	gene expression real-time qpcr cancer-testicular antigens (cta) breast cancer immunotherapy Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/88cf9366e4b0431181328b35e86d63c2
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spelling	oai:doaj.org-article:88cf9366e4b0431181328b35e86d63c22021-11-18T08:03:47ZTRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER1563-06252313-741X10.15789/1563-0625-2018-3-383-390https://doaj.org/article/88cf9366e4b0431181328b35e86d63c22018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1542https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XTo identify new effective targets for immunotherapy of breast cancer, based on cancer-testis antigens (CTA), the associations were studied between CTA gene expression, clinical and pathological characteristics of breast cancer. Therefore, the aim of the study was to perform screening of CTAs specific to breast tissue tumors (luminal types A and B) based on assessment of the transcriptional profile of cancer-testis genes in the patients of different ages. To evaluate these relations, paired surgical biopsies (normal and tumor) of breast tissue of 32 patients (64 samples) aged 38-86 years were used. Relative expression of 16 genetic loci (MAGEA1, MAGEA2, MAGEA3, MAGEA4, MAGEB1, MAGEB2, GAGE1, GAGE3, GAGE4, MAGEC1, BAGE, XAGE3, NY-ESO1, SSX2, SYCP1 and PRAME1) was determined by the RT-qPCR. It was found that the transcriptional profile of CTA differs in different age groups of patients (at the age of < 55 years, overexpression of MAGEA3 was noted; after 55 years, MAGEA1, MAGEB1, BAGE, NY-ESO1, GAGE1 and GAGE3 were more expressed). In the luminal type A cancer, overexpression of MAGEA1, MAGEA2, MAGEA4, MAGEB1, MAGEB2, GAGE3, GAGE4, MAGEC1 and PRAME1, whereas in B type cancer, the genes with increased expression were not detected. These differences should be considered when planning immunotherapy, can be used as biomarkers for both breast cancer as clinical entity, and, in particular, for its individual subtypes.D. I. VodolazhskiyD. S. KutilinKh. A. MogushkovaO. I. KitSPb RAACIarticlegene expressionreal-time qpcrcancer-testicular antigens (cta)breast cancerimmunotherapyImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 3, Pp 383-390 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	gene expression real-time qpcr cancer-testicular antigens (cta) breast cancer immunotherapy Immunologic diseases. Allergy RC581-607
spellingShingle	gene expression real-time qpcr cancer-testicular antigens (cta) breast cancer immunotherapy Immunologic diseases. Allergy RC581-607 D. I. Vodolazhskiy D. S. Kutilin Kh. A. Mogushkova O. I. Kit TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
description	To identify new effective targets for immunotherapy of breast cancer, based on cancer-testis antigens (CTA), the associations were studied between CTA gene expression, clinical and pathological characteristics of breast cancer. Therefore, the aim of the study was to perform screening of CTAs specific to breast tissue tumors (luminal types A and B) based on assessment of the transcriptional profile of cancer-testis genes in the patients of different ages. To evaluate these relations, paired surgical biopsies (normal and tumor) of breast tissue of 32 patients (64 samples) aged 38-86 years were used. Relative expression of 16 genetic loci (MAGEA1, MAGEA2, MAGEA3, MAGEA4, MAGEB1, MAGEB2, GAGE1, GAGE3, GAGE4, MAGEC1, BAGE, XAGE3, NY-ESO1, SSX2, SYCP1 and PRAME1) was determined by the RT-qPCR. It was found that the transcriptional profile of CTA differs in different age groups of patients (at the age of < 55 years, overexpression of MAGEA3 was noted; after 55 years, MAGEA1, MAGEB1, BAGE, NY-ESO1, GAGE1 and GAGE3 were more expressed). In the luminal type A cancer, overexpression of MAGEA1, MAGEA2, MAGEA4, MAGEB1, MAGEB2, GAGE3, GAGE4, MAGEC1 and PRAME1, whereas in B type cancer, the genes with increased expression were not detected. These differences should be considered when planning immunotherapy, can be used as biomarkers for both breast cancer as clinical entity, and, in particular, for its individual subtypes.
format	article
author	D. I. Vodolazhskiy D. S. Kutilin Kh. A. Mogushkova O. I. Kit
author_facet	D. I. Vodolazhskiy D. S. Kutilin Kh. A. Mogushkova O. I. Kit
author_sort	D. I. Vodolazhskiy
title	TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
title_short	TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
title_full	TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
title_fullStr	TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
title_full_unstemmed	TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER
title_sort	transcriptional profile of cancer-testicular antigens in patients with breast cancer
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/88cf9366e4b0431181328b35e86d63c2
work_keys_str_mv	AT divodolazhskiy transcriptionalprofileofcancertesticularantigensinpatientswithbreastcancer AT dskutilin transcriptionalprofileofcancertesticularantigensinpatientswithbreastcancer AT khamogushkova transcriptionalprofileofcancertesticularantigensinpatientswithbreastcancer AT oikit transcriptionalprofileofcancertesticularantigensinpatientswithbreastcancer
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TRANSCRIPTIONAL PROFILE OF CANCER-TESTICULAR ANTIGENS IN PATIENTS WITH BREAST CANCER

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