Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System
<i>Dittrichia viscosa</i> plants were grown hydroponically with different concentrations of Sb. There was preferential accumulation of Sb in roots. Fe and Cu decreased, while Mn decreased in roots but not in leaves. Chlorophyll content declined, but the carotenoid content increased, and...
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2021
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oai:doaj.org-article:88d066a638944c2687daebf791402e072021-11-25T16:26:29ZResponse to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System10.3390/antiox101116982076-3921https://doaj.org/article/88d066a638944c2687daebf791402e072021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1698https://doaj.org/toc/2076-3921<i>Dittrichia viscosa</i> plants were grown hydroponically with different concentrations of Sb. There was preferential accumulation of Sb in roots. Fe and Cu decreased, while Mn decreased in roots but not in leaves. Chlorophyll content declined, but the carotenoid content increased, and photosynthetic efficiency was unaltered. O<sub>2</sub><sup>●−</sup> generation increased slightly, while lipid peroxidation increased only in roots. H<sub>2</sub>O<sub>2</sub>, NO, ONOO<sup>−</sup>, S-nitrosothiols, and H<sub>2</sub>S showed significant increases, and the enzymatic antioxidant system was altered. In roots, superoxide dismutase (SOD) and monodehydroascorbate reductase (MDAR) activities declined, dehydroscorbate reductase (DHAR) rose, and ascorbate peroxidase (APX), peroxidase (POX), and glutathione reductase (GR) were unaffected. In leaves, SOD and POX increased, MDAR decreased, and APX was unaltered, while GR increased. S-nitrosoglutathione reductase (GSNOR) and <span style="font-variant: small-caps;">l</span>-cysteine desulfhydrilase (<span style="font-variant: small-caps;">l</span>-DES) increased in activity, while glutathione S-transferase (GST) decreased in leaves but was enhanced in roots. Components of the AsA/GSH cycle decreased. The great capacity of <i>Dittrichia</i> roots to accumulate Sb is the reason for the differing behaviour observed in the enzymatic antioxidant systems of the two organs. Sb appears to act by binding to thiol groups, which can alter free GSH content and SOD and GST activities. The coniferyl alcohol peroxidase activity increased, possibly to lignify the roots’ cell walls. Sb altered the ROS balance, especially with respect to H<sub>2</sub>O<sub>2</sub>. This led to an increase in NO and H<sub>2</sub>S acting on the antioxidant system to limit that Sb-induced redox imbalance. The interaction NO, H<sub>2</sub>S and H<sub>2</sub>O<sub>2</sub> appears key to the response to stress induced by Sb. The interaction between ROS, NO, and H<sub>2</sub>S appears to be involved in the response to Sb.Francisco Luis Espinosa-VellarinoInmaculada GarridoAlfonso OrtegaIlda CasimiroFrancisco EspinosaMDPI AGarticleantimonyAsA/GSH<i>Dittrichia</i>hydrogen sulfidenitric oxidereactive oxygen speciesTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1698, p 1698 (2021) |
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DOAJ |
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EN |
| topic |
antimony AsA/GSH <i>Dittrichia</i> hydrogen sulfide nitric oxide reactive oxygen species Therapeutics. Pharmacology RM1-950 |
| spellingShingle |
antimony AsA/GSH <i>Dittrichia</i> hydrogen sulfide nitric oxide reactive oxygen species Therapeutics. Pharmacology RM1-950 Francisco Luis Espinosa-Vellarino Inmaculada Garrido Alfonso Ortega Ilda Casimiro Francisco Espinosa Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| description |
<i>Dittrichia viscosa</i> plants were grown hydroponically with different concentrations of Sb. There was preferential accumulation of Sb in roots. Fe and Cu decreased, while Mn decreased in roots but not in leaves. Chlorophyll content declined, but the carotenoid content increased, and photosynthetic efficiency was unaltered. O<sub>2</sub><sup>●−</sup> generation increased slightly, while lipid peroxidation increased only in roots. H<sub>2</sub>O<sub>2</sub>, NO, ONOO<sup>−</sup>, S-nitrosothiols, and H<sub>2</sub>S showed significant increases, and the enzymatic antioxidant system was altered. In roots, superoxide dismutase (SOD) and monodehydroascorbate reductase (MDAR) activities declined, dehydroscorbate reductase (DHAR) rose, and ascorbate peroxidase (APX), peroxidase (POX), and glutathione reductase (GR) were unaffected. In leaves, SOD and POX increased, MDAR decreased, and APX was unaltered, while GR increased. S-nitrosoglutathione reductase (GSNOR) and <span style="font-variant: small-caps;">l</span>-cysteine desulfhydrilase (<span style="font-variant: small-caps;">l</span>-DES) increased in activity, while glutathione S-transferase (GST) decreased in leaves but was enhanced in roots. Components of the AsA/GSH cycle decreased. The great capacity of <i>Dittrichia</i> roots to accumulate Sb is the reason for the differing behaviour observed in the enzymatic antioxidant systems of the two organs. Sb appears to act by binding to thiol groups, which can alter free GSH content and SOD and GST activities. The coniferyl alcohol peroxidase activity increased, possibly to lignify the roots’ cell walls. Sb altered the ROS balance, especially with respect to H<sub>2</sub>O<sub>2</sub>. This led to an increase in NO and H<sub>2</sub>S acting on the antioxidant system to limit that Sb-induced redox imbalance. The interaction NO, H<sub>2</sub>S and H<sub>2</sub>O<sub>2</sub> appears key to the response to stress induced by Sb. The interaction between ROS, NO, and H<sub>2</sub>S appears to be involved in the response to Sb. |
| format |
article |
| author |
Francisco Luis Espinosa-Vellarino Inmaculada Garrido Alfonso Ortega Ilda Casimiro Francisco Espinosa |
| author_facet |
Francisco Luis Espinosa-Vellarino Inmaculada Garrido Alfonso Ortega Ilda Casimiro Francisco Espinosa |
| author_sort |
Francisco Luis Espinosa-Vellarino |
| title |
Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| title_short |
Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| title_full |
Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| title_fullStr |
Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| title_full_unstemmed |
Response to Antimony Toxicity in <i>Dittrichia viscosa</i> Plants: ROS, NO, H<sub>2</sub>S, and the Antioxidant System |
| title_sort |
response to antimony toxicity in <i>dittrichia viscosa</i> plants: ros, no, h<sub>2</sub>s, and the antioxidant system |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/88d066a638944c2687daebf791402e07 |
| work_keys_str_mv |
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| _version_ |
1718413210595360768 |