Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.

The worldwide rise in the rates of antibiotic resistance of bacteria underlines the need for alternative antibacterial agents. A promising approach to kill antibiotic-resistant bacteria uses light in combination with a photosensitizer to induce a phototoxic reaction. Concentrations of 1, 10 and 100m...

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Autores principales:	Stanislaw Schastak, Svitlana Ziganshyna, Burkhard Gitter, Peter Wiedemann, Thomas Claudepierre
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88d0c6090f934260beaf83f1cfd9f9bd
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spelling	oai:doaj.org-article:88d0c6090f934260beaf83f1cfd9f9bd2021-12-02T20:19:56ZEfficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.1932-620310.1371/journal.pone.0011674https://doaj.org/article/88d0c6090f934260beaf83f1cfd9f9bd2010-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20652031/?tool=EBIhttps://doaj.org/toc/1932-6203The worldwide rise in the rates of antibiotic resistance of bacteria underlines the need for alternative antibacterial agents. A promising approach to kill antibiotic-resistant bacteria uses light in combination with a photosensitizer to induce a phototoxic reaction. Concentrations of 1, 10 and 100microM of tetrahydroporphyrin-tetratosylat (THPTS) and different incubation times (30, 90 and 180min) were used to measure photodynamic efficiency against two Gram-positive strains of S.aureus (MSSA and MRSA), and two Gram-negative strains of E.coli and P.aeruginosa. We found that phototoxicity of the drug is independent of the antibiotic resistance pattern when incubated in PBS for the investigated strains. Also, an incubation with 100microM THPTS followed by illumination, yielded a 6lg (> or =99.999%) decrease in the viable numbers of all bacteria strains tested, indicating that the THPTS drug has a high degree of photodynamic inactivation. We then modulated incubation time, photosensitizer concentration and monitored the effect of serum on the THPTS activity. In doing so, we established the conditions to obtain the strongest bactericidal effect. Our results suggest that this new and highly pure synthetic compound should improve the efficiency of photodynamic therapy against multiresistant bacteria and has a significant potential for clinical applications in the treatment of nosocomial infections.Stanislaw SchastakSvitlana ZiganshynaBurkhard GitterPeter WiedemannThomas ClaudepierrePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 7, p e11674 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stanislaw Schastak Svitlana Ziganshyna Burkhard Gitter Peter Wiedemann Thomas Claudepierre Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
description	The worldwide rise in the rates of antibiotic resistance of bacteria underlines the need for alternative antibacterial agents. A promising approach to kill antibiotic-resistant bacteria uses light in combination with a photosensitizer to induce a phototoxic reaction. Concentrations of 1, 10 and 100microM of tetrahydroporphyrin-tetratosylat (THPTS) and different incubation times (30, 90 and 180min) were used to measure photodynamic efficiency against two Gram-positive strains of S.aureus (MSSA and MRSA), and two Gram-negative strains of E.coli and P.aeruginosa. We found that phototoxicity of the drug is independent of the antibiotic resistance pattern when incubated in PBS for the investigated strains. Also, an incubation with 100microM THPTS followed by illumination, yielded a 6lg (> or =99.999%) decrease in the viable numbers of all bacteria strains tested, indicating that the THPTS drug has a high degree of photodynamic inactivation. We then modulated incubation time, photosensitizer concentration and monitored the effect of serum on the THPTS activity. In doing so, we established the conditions to obtain the strongest bactericidal effect. Our results suggest that this new and highly pure synthetic compound should improve the efficiency of photodynamic therapy against multiresistant bacteria and has a significant potential for clinical applications in the treatment of nosocomial infections.
format	article
author	Stanislaw Schastak Svitlana Ziganshyna Burkhard Gitter Peter Wiedemann Thomas Claudepierre
author_facet	Stanislaw Schastak Svitlana Ziganshyna Burkhard Gitter Peter Wiedemann Thomas Claudepierre
author_sort	Stanislaw Schastak
title	Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
title_short	Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
title_full	Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
title_fullStr	Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
title_full_unstemmed	Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.
title_sort	efficient photodynamic therapy against gram-positive and gram-negative bacteria using thpts, a cationic photosensitizer excited by infrared wavelength.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/88d0c6090f934260beaf83f1cfd9f9bd
work_keys_str_mv	AT stanislawschastak efficientphotodynamictherapyagainstgrampositiveandgramnegativebacteriausingthptsacationicphotosensitizerexcitedbyinfraredwavelength AT svitlanaziganshyna efficientphotodynamictherapyagainstgrampositiveandgramnegativebacteriausingthptsacationicphotosensitizerexcitedbyinfraredwavelength AT burkhardgitter efficientphotodynamictherapyagainstgrampositiveandgramnegativebacteriausingthptsacationicphotosensitizerexcitedbyinfraredwavelength AT peterwiedemann efficientphotodynamictherapyagainstgrampositiveandgramnegativebacteriausingthptsacationicphotosensitizerexcitedbyinfraredwavelength AT thomasclaudepierre efficientphotodynamictherapyagainstgrampositiveandgramnegativebacteriausingthptsacationicphotosensitizerexcitedbyinfraredwavelength
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Efficient photodynamic therapy against gram-positive and gram-negative bacteria using THPTS, a cationic photosensitizer excited by infrared wavelength.

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