Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: Aspergillus terreus and Streptomyces rimosus Warfare in Bioreactors

The aim of this study was to quantitatively characterize the morphology of the filamentous microorganisms Aspergillus terreus ATCC 20542 and Streptomyces rimosus ATCC 10970, cocultivated in stirred tank bioreactors, and to characterize their mutual influence wit...

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Autores principales:	Anna Ścigaczewska, Tomasz Boruta, Marcin Bizukojć
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cocultures filamentous microorganisms morphology image analysis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/88d115e533554bc4a3066fc0ff8fd124
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spelling	oai:doaj.org-article:88d115e533554bc4a3066fc0ff8fd1242021-11-25T16:54:45ZQuantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors10.3390/biom111117402218-273Xhttps://doaj.org/article/88d115e533554bc4a3066fc0ff8fd1242021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1740https://doaj.org/toc/2218-273XThe aim of this study was to quantitatively characterize the morphology of the filamentous microorganisms <i>Aspergillus terreus</i> ATCC 20542 and <i>Streptomyces rimosus</i> ATCC 10970, cocultivated in stirred tank bioreactors, and to characterize their mutual influence with the use of quantitative image analysis. Three distinct coculture initiation strategies were applied: preculture versus preculture, spores versus spores and preculture versus preculture with time delay for one of the species. Bioreactor cocultures were accompanied by parallel monoculture controls. The results recorded for the mono- and cocultures were compared in order to investigate the effect of cocultivation on the morphological evolution of <i>A. terreus</i> and <i>S. rimosus</i>. Morphology-related observations were also confronted with the analysis of secondary metabolism. The morphology of the two studied filamentous species strictly depended on the applied coculture initiation strategy. In the cocultures initiated by the simultaneous inoculation, <i>S. rimosus</i> gained domination or advance over <i>A. terreus</i>. The latter microorganism dominated only in these experiments in which <i>S. rimosus</i> was introduced with a delay.Anna ŚcigaczewskaTomasz BorutaMarcin BizukojćMDPI AGarticlecoculturesfilamentous microorganismsmorphologyimage analysisMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1740, p 1740 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cocultures filamentous microorganisms morphology image analysis Microbiology QR1-502
spellingShingle	cocultures filamentous microorganisms morphology image analysis Microbiology QR1-502 Anna Ścigaczewska Tomasz Boruta Marcin Bizukojć Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
description	The aim of this study was to quantitatively characterize the morphology of the filamentous microorganisms <i>Aspergillus terreus</i> ATCC 20542 and <i>Streptomyces rimosus</i> ATCC 10970, cocultivated in stirred tank bioreactors, and to characterize their mutual influence with the use of quantitative image analysis. Three distinct coculture initiation strategies were applied: preculture versus preculture, spores versus spores and preculture versus preculture with time delay for one of the species. Bioreactor cocultures were accompanied by parallel monoculture controls. The results recorded for the mono- and cocultures were compared in order to investigate the effect of cocultivation on the morphological evolution of <i>A. terreus</i> and <i>S. rimosus</i>. Morphology-related observations were also confronted with the analysis of secondary metabolism. The morphology of the two studied filamentous species strictly depended on the applied coculture initiation strategy. In the cocultures initiated by the simultaneous inoculation, <i>S. rimosus</i> gained domination or advance over <i>A. terreus</i>. The latter microorganism dominated only in these experiments in which <i>S. rimosus</i> was introduced with a delay.
format	article
author	Anna Ścigaczewska Tomasz Boruta Marcin Bizukojć
author_facet	Anna Ścigaczewska Tomasz Boruta Marcin Bizukojć
author_sort	Anna Ścigaczewska
title	Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
title_short	Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
title_full	Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
title_fullStr	Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
title_full_unstemmed	Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors
title_sort	quantitative morphological analysis of filamentous microorganisms in cocultures and monocultures: <i>aspergillus terreus</i> and <i>streptomyces rimosus</i> warfare in bioreactors
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/88d115e533554bc4a3066fc0ff8fd124
work_keys_str_mv	AT annascigaczewska quantitativemorphologicalanalysisoffilamentousmicroorganismsincoculturesandmonoculturesiaspergillusterreusiandistreptomycesrimosusiwarfareinbioreactors AT tomaszboruta quantitativemorphologicalanalysisoffilamentousmicroorganismsincoculturesandmonoculturesiaspergillusterreusiandistreptomycesrimosusiwarfareinbioreactors AT marcinbizukojc quantitativemorphologicalanalysisoffilamentousmicroorganismsincoculturesandmonoculturesiaspergillusterreusiandistreptomycesrimosusiwarfareinbioreactors
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Quantitative Morphological Analysis of Filamentous Microorganisms in Cocultures and Monocultures: <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> Warfare in Bioreactors

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