ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS

Nanotechnology in immunology is a prospectively developing area in fundamental and practical medicine. Cucurbiturils are macrocyclic cavitands with a definite amount of glycoluril fragments (n) that determine the size of the cavity of these compounds. Nowadays, there are six synthesized homologues:...

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Autores principales:	A. A. Aktanova, E. A. Pashkina, V. A. Kozlov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	cucurbiturils macrocyclic compounds immunity innate immune system monocytes nk-cells nkt-cells flow cytometry Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/88e1dbc0f3a54f18afe21be79e04e1a1
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spelling	oai:doaj.org-article:88e1dbc0f3a54f18afe21be79e04e1a12021-11-18T08:03:51ZASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS1563-06252313-741X10.15789/1563-0625-AEO-2244https://doaj.org/article/88e1dbc0f3a54f18afe21be79e04e1a12021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2244https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XNanotechnology in immunology is a prospectively developing area in fundamental and practical medicine. Cucurbiturils are macrocyclic cavitands with a definite amount of glycoluril fragments (n) that determine the size of the cavity of these compounds. Nowadays, there are six synthesized homologues: 5, 6, 7, 8, 10 and 14. They differ from each other in the portal size and the size of the cavities. They are characterized by special physicochemical and biological properties, such as biocompatibility, stability, high ability to encapsulate chemical compounds. It is known that cucurbiturils encapsulate molecules by forming guest-host complexes, which allow the substance to be released from the complex and increase the solubility of the compounds. These advantages allow using cucurbiturils as drug delivery systems. Immunomodulatory activity of cucurbiturils depends on its specific nanoscale characteristics: functional groups, shape, size, surface, solubility in various media. Each nanoparticle depending on these properties has different effects on cells. The effects of cucurbiturils can be different even for one subpopulation of cells, depending on the homologue or dosage. The interaction of innate immune cells with cucurbiturils are not yet sufficiently characterized.The aim of this study was to assess the effects of cucurbit[n]urils (n = 6, n = 7, n = 8) on innate immune cells – monocytes, NK-cells, NKT-cells.The immunological recearch included the isolation of peripheral blood mononuclear cells from healthy donors (n = 8) on the density gradient of ficoll-urografin and flow cytometry with the determination of the amount of immunocompetent cells according to the classic markers of differentiation of these cells – CD3- CD16+CD56+ for NK-cells, CD3+CD16+CD56+ for NKT-cells and CD3- CD14+ for monocytes. Monocyte activation was determined by the expression of surface HLA-DR.The cells were cultured for 72 hours with the addition of cucurbiturils CB[6], CB[7] at concentrations of 0.1 mM, 0.3 mM, 0.5 mM and CB[8] at concentration of 0.01 mM, due to its poor solubility.There were a significant decrease in the quantity of NK-cells (p < 0.01 for the test concentrations of CB[7]), an increase in the quantity of NKT-cells (p < 0.04 and p < 0.02 respectively for the concentrations of CB[6] and CB[7]). There was a tendency to increase the expression of HLA-DR on monocytes (p = 0.06 for CB[6]).Considering a variative effects of cucurbiturils, in the future it is possible to consider a possibility of using cucurbiturils as an immunomodulators, antitumor agents, in autoimmune diseases.A. A. AktanovaE. A. PashkinaV. A. KozlovSPb RAACIarticlecucurbiturilsmacrocyclic compoundsimmunityinnate immune systemmonocytesnk-cellsnkt-cellsflow cytometryImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 635-640 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	cucurbiturils macrocyclic compounds immunity innate immune system monocytes nk-cells nkt-cells flow cytometry Immunologic diseases. Allergy RC581-607
spellingShingle	cucurbiturils macrocyclic compounds immunity innate immune system monocytes nk-cells nkt-cells flow cytometry Immunologic diseases. Allergy RC581-607 A. A. Aktanova E. A. Pashkina V. A. Kozlov ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
description	Nanotechnology in immunology is a prospectively developing area in fundamental and practical medicine. Cucurbiturils are macrocyclic cavitands with a definite amount of glycoluril fragments (n) that determine the size of the cavity of these compounds. Nowadays, there are six synthesized homologues: 5, 6, 7, 8, 10 and 14. They differ from each other in the portal size and the size of the cavities. They are characterized by special physicochemical and biological properties, such as biocompatibility, stability, high ability to encapsulate chemical compounds. It is known that cucurbiturils encapsulate molecules by forming guest-host complexes, which allow the substance to be released from the complex and increase the solubility of the compounds. These advantages allow using cucurbiturils as drug delivery systems. Immunomodulatory activity of cucurbiturils depends on its specific nanoscale characteristics: functional groups, shape, size, surface, solubility in various media. Each nanoparticle depending on these properties has different effects on cells. The effects of cucurbiturils can be different even for one subpopulation of cells, depending on the homologue or dosage. The interaction of innate immune cells with cucurbiturils are not yet sufficiently characterized.The aim of this study was to assess the effects of cucurbit[n]urils (n = 6, n = 7, n = 8) on innate immune cells – monocytes, NK-cells, NKT-cells.The immunological recearch included the isolation of peripheral blood mononuclear cells from healthy donors (n = 8) on the density gradient of ficoll-urografin and flow cytometry with the determination of the amount of immunocompetent cells according to the classic markers of differentiation of these cells – CD3- CD16+CD56+ for NK-cells, CD3+CD16+CD56+ for NKT-cells and CD3- CD14+ for monocytes. Monocyte activation was determined by the expression of surface HLA-DR.The cells were cultured for 72 hours with the addition of cucurbiturils CB[6], CB[7] at concentrations of 0.1 mM, 0.3 mM, 0.5 mM and CB[8] at concentration of 0.01 mM, due to its poor solubility.There were a significant decrease in the quantity of NK-cells (p < 0.01 for the test concentrations of CB[7]), an increase in the quantity of NKT-cells (p < 0.04 and p < 0.02 respectively for the concentrations of CB[6] and CB[7]). There was a tendency to increase the expression of HLA-DR on monocytes (p = 0.06 for CB[6]).Considering a variative effects of cucurbiturils, in the future it is possible to consider a possibility of using cucurbiturils as an immunomodulators, antitumor agents, in autoimmune diseases.
format	article
author	A. A. Aktanova E. A. Pashkina V. A. Kozlov
author_facet	A. A. Aktanova E. A. Pashkina V. A. Kozlov
author_sort	A. A. Aktanova
title	ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
title_short	ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
title_full	ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
title_fullStr	ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
title_full_unstemmed	ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS
title_sort	assessing effects of cucurbiturils on monocytes and nk-cells in healthy volunteers
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/88e1dbc0f3a54f18afe21be79e04e1a1
work_keys_str_mv	AT aaaktanova assessingeffectsofcucurbiturilsonmonocytesandnkcellsinhealthyvolunteers AT eapashkina assessingeffectsofcucurbiturilsonmonocytesandnkcellsinhealthyvolunteers AT vakozlov assessingeffectsofcucurbiturilsonmonocytesandnkcellsinhealthyvolunteers
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ASSESSING EFFECTS OF CUCURBITURILS ON MONOCYTES AND NK-CELLS IN HEALTHY VOLUNTEERS

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