Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.

The bean bug Riptortus pedestris possesses a specialized symbiotic organ in a posterior region of the midgut, where numerous crypts harbor extracellular betaproteobacterial symbionts of the genus Burkholderia. Second instar nymphs orally acquire the symbiont from the environment, and the symbiont in...

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Autores principales:	Ryo Futahashi, Kohjiro Tanaka, Masahiko Tanahashi, Naruo Nikoh, Yoshitomo Kikuchi, Bok Luel Lee, Takema Fukatsu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88e2d6fb13c84a90bb62dd2eec8b680f
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spelling	oai:doaj.org-article:88e2d6fb13c84a90bb62dd2eec8b680f2021-11-18T07:45:44ZGene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.1932-620310.1371/journal.pone.0064557https://doaj.org/article/88e2d6fb13c84a90bb62dd2eec8b680f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23691247/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The bean bug Riptortus pedestris possesses a specialized symbiotic organ in a posterior region of the midgut, where numerous crypts harbor extracellular betaproteobacterial symbionts of the genus Burkholderia. Second instar nymphs orally acquire the symbiont from the environment, and the symbiont infection benefits the host by facilitating growth and by occasionally conferring insecticide resistance. Here we performed comparative transcriptomic analyses of insect genes expressed in symbiotic and non-symbiotic regions of the midgut dissected from Burkholderia-infected and uninfected R. pedestris. Expression sequence tag analysis of cDNA libraries and quantitative reverse transcription PCR identified a number of insect genes expressed in symbiosis- or aposymbiosis-associated patterns. For example, genes up-regulated in symbiotic relative to aposymbiotic individuals, including many cysteine-rich secreted protein genes and many cathepsin protease genes, are likely to play a role in regulating the symbiosis. Conversely, genes up-regulated in aposymbiotic relative to symbiotic individuals, including a chicken-type lysozyme gene and a defensin-like protein gene, are possibly involved in regulation of non-symbiotic bacterial infections. Our study presents the first transcriptomic data on gut symbiotic organ of a stinkbug, which provides initial clues to understanding of molecular mechanisms underlying the insect-bacterium gut symbiosis and sheds light on several intriguing commonalities between endocellular and extracellular symbiotic associations.Ryo FutahashiKohjiro TanakaMasahiko TanahashiNaruo NikohYoshitomo KikuchiBok Luel LeeTakema FukatsuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64557 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryo Futahashi Kohjiro Tanaka Masahiko Tanahashi Naruo Nikoh Yoshitomo Kikuchi Bok Luel Lee Takema Fukatsu Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
description	The bean bug Riptortus pedestris possesses a specialized symbiotic organ in a posterior region of the midgut, where numerous crypts harbor extracellular betaproteobacterial symbionts of the genus Burkholderia. Second instar nymphs orally acquire the symbiont from the environment, and the symbiont infection benefits the host by facilitating growth and by occasionally conferring insecticide resistance. Here we performed comparative transcriptomic analyses of insect genes expressed in symbiotic and non-symbiotic regions of the midgut dissected from Burkholderia-infected and uninfected R. pedestris. Expression sequence tag analysis of cDNA libraries and quantitative reverse transcription PCR identified a number of insect genes expressed in symbiosis- or aposymbiosis-associated patterns. For example, genes up-regulated in symbiotic relative to aposymbiotic individuals, including many cysteine-rich secreted protein genes and many cathepsin protease genes, are likely to play a role in regulating the symbiosis. Conversely, genes up-regulated in aposymbiotic relative to symbiotic individuals, including a chicken-type lysozyme gene and a defensin-like protein gene, are possibly involved in regulation of non-symbiotic bacterial infections. Our study presents the first transcriptomic data on gut symbiotic organ of a stinkbug, which provides initial clues to understanding of molecular mechanisms underlying the insect-bacterium gut symbiosis and sheds light on several intriguing commonalities between endocellular and extracellular symbiotic associations.
format	article
author	Ryo Futahashi Kohjiro Tanaka Masahiko Tanahashi Naruo Nikoh Yoshitomo Kikuchi Bok Luel Lee Takema Fukatsu
author_facet	Ryo Futahashi Kohjiro Tanaka Masahiko Tanahashi Naruo Nikoh Yoshitomo Kikuchi Bok Luel Lee Takema Fukatsu
author_sort	Ryo Futahashi
title	Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
title_short	Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
title_full	Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
title_fullStr	Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
title_full_unstemmed	Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
title_sort	gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/88e2d6fb13c84a90bb62dd2eec8b680f
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Gene expression in gut symbiotic organ of stinkbug affected by extracellular bacterial symbiont.

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