Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens

Abstract The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O2-limited conditions were studied. NADH respiration in isolated membrane particles were par...

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Autores principales:	Rubén D. Jaramillo-Lanchero, Paola Suarez-Alvarez, Luis Teheran-Sierra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/88e6674af31a41af88ec4179eec36275
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spelling	oai:doaj.org-article:88e6674af31a41af88ec4179eec362752021-12-02T17:39:20ZEffect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens10.1038/s41598-021-88388-02045-2322https://doaj.org/article/88e6674af31a41af88ec4179eec362752021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88388-0https://doaj.org/toc/2045-2322Abstract The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O2-limited conditions were studied. NADH respiration in isolated membrane particles were partially inhibited by rotenone, dicoumarol, quinacrine, flavone, and capsaicin. A similar response was obtained when succinate oxidation was performed in the presence of thenoyltrifluoroacetone and N,N’-dicyclohexylcarbodiimide. NADH respiration was resistant to site II inhibitors and cyanide, indicating that a percentage of the electrons transported can reach O2 without the bc 1 complex. Succinate respiration was sensitive to myxothiazol, antimycin A and 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Juglone, plumbagin and menadione had higher reactivity with NADH dehydrogenase. The membrane particles showed the highest oxidase activities with ascorbate-TCHQ (tetrachlorohydroquinone), TCHQ alone, and NADH-TMPD (N,N,N’,N’-tetramethyl-p-phenylenediamine), and minor activity levels with ascorbate-DCPIP (2,6-dichloro-phenolindophenol) and NADH-DCPIP. The substrates NADH-DCPIP, NADH-TMPD and TCHQ were electron donors to cyanide-sensitive cbb' cytochrome c oxidase. The presence of dissimilatory nitrate reductase in the aerobic respiratory system of E. corrodens ATCC 23834 was demonstrated by first time. Our results indicate that complexes I and II have resistance to their classic inhibitors, that the oxidation of NADH is stimulated by juglone, plumbagin and menadione, and that sensitivity to KCN is stimulated by the substrates TCHQ, NADH-DCPIP and NADH-TMPD.Rubén D. Jaramillo-LancheroPaola Suarez-AlvarezLuis Teheran-SierraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rubén D. Jaramillo-Lanchero Paola Suarez-Alvarez Luis Teheran-Sierra Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
description	Abstract The effects of respiratory inhibitors, quinone analogues and artificial substrates on the membrane-bound electron transport system of the fastidious β-proteobacterium Eikenella corrodens grown under O2-limited conditions were studied. NADH respiration in isolated membrane particles were partially inhibited by rotenone, dicoumarol, quinacrine, flavone, and capsaicin. A similar response was obtained when succinate oxidation was performed in the presence of thenoyltrifluoroacetone and N,N’-dicyclohexylcarbodiimide. NADH respiration was resistant to site II inhibitors and cyanide, indicating that a percentage of the electrons transported can reach O2 without the bc 1 complex. Succinate respiration was sensitive to myxothiazol, antimycin A and 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO). Juglone, plumbagin and menadione had higher reactivity with NADH dehydrogenase. The membrane particles showed the highest oxidase activities with ascorbate-TCHQ (tetrachlorohydroquinone), TCHQ alone, and NADH-TMPD (N,N,N’,N’-tetramethyl-p-phenylenediamine), and minor activity levels with ascorbate-DCPIP (2,6-dichloro-phenolindophenol) and NADH-DCPIP. The substrates NADH-DCPIP, NADH-TMPD and TCHQ were electron donors to cyanide-sensitive cbb' cytochrome c oxidase. The presence of dissimilatory nitrate reductase in the aerobic respiratory system of E. corrodens ATCC 23834 was demonstrated by first time. Our results indicate that complexes I and II have resistance to their classic inhibitors, that the oxidation of NADH is stimulated by juglone, plumbagin and menadione, and that sensitivity to KCN is stimulated by the substrates TCHQ, NADH-DCPIP and NADH-TMPD.
format	article
author	Rubén D. Jaramillo-Lanchero Paola Suarez-Alvarez Luis Teheran-Sierra
author_facet	Rubén D. Jaramillo-Lanchero Paola Suarez-Alvarez Luis Teheran-Sierra
author_sort	Rubén D. Jaramillo-Lanchero
title	Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
title_short	Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
title_full	Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
title_fullStr	Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
title_full_unstemmed	Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens
title_sort	effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of eikenella corrodens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/88e6674af31a41af88ec4179eec36275
work_keys_str_mv	AT rubendjaramillolanchero effectofrespiratoryinhibitorsandquinoneanaloguesontheaerobicelectrontransportsystemofeikenellacorrodens AT paolasuarezalvarez effectofrespiratoryinhibitorsandquinoneanaloguesontheaerobicelectrontransportsystemofeikenellacorrodens AT luisteheransierra effectofrespiratoryinhibitorsandquinoneanaloguesontheaerobicelectrontransportsystemofeikenellacorrodens
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Effect of respiratory inhibitors and quinone analogues on the aerobic electron transport system of Eikenella corrodens

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