Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans

Shuang Liang,1,2,* Junchao Duan,1,2,* Hejing Hu,1,2 Jingyi Zhang,1,2 Shan Gao,2,3 Haiming Jing,2,3 Guojun Li,2,3 Zhiwei Sun1,2 1Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, People’s Republic of China; 2Beijing Key Lab...

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Autores principales:	Liang S, Duan J, Hu H, Zhang J, Gao S, Jing H, Li G, Sun Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	sinps genome microarrays longevity development c. elegans Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/88e8d3de30da41cabd92d5b3172f57f3
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spelling	oai:doaj.org-article:88e8d3de30da41cabd92d5b3172f57f32021-12-02T12:21:55ZComprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans1178-2013https://doaj.org/article/88e8d3de30da41cabd92d5b3172f57f32020-07-01T00:00:00Zhttps://www.dovepress.com/comprehensive-analysis-of-sinps-on-the-genome-wide-transcriptional-cha-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shuang Liang,1,2,* Junchao Duan,1,2,* Hejing Hu,1,2 Jingyi Zhang,1,2 Shan Gao,2,3 Haiming Jing,2,3 Guojun Li,2,3 Zhiwei Sun1,2 1Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, People’s Republic of China; 2Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, People’s Republic of China; 3Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiwei SunSchool of Public Health, Capital Medical University, Beijing 100069, People’s Republic of ChinaEmail zwsun@ccmu.edu.cnGuojun LiBeijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of ChinaEmail ligj@bjcdc.orgBackground: Large-scale production and application of amorphous silica nanoparticles (SiNPs) have enhanced the risk of human exposure to SiNPs. However, the toxic effects and the underlying biological mechanisms of SiNPs on Caenorhabditis elegans remain largely unclear.Purpose: This study was to investigate the genome-wide transcriptional alteration of SiNPs on C. elegans.Methods and Results: In this study, a total number of 3105 differentially expressed genes were identified in C. elegans. Among them, 1398 genes were significantly upregulated and 1707 genes were notably downregulated in C. elegans. Gene ontology analysis revealed that the significant change of gene functional categories triggered by SiNPs was focused on locomotion, determination of adult lifespan, reproduction, body morphogenesis, multicellular organism development, endoplasmic reticulum unfolded protein response, oocyte development, and nematode larval development. Meanwhile, we explored the regulated effects between microRNA and genes or signaling pathways. Pathway enrichment analysis and miRNA-gene-pathway-network displayed that 23 differential expression microRNA including cel-miR-85-3p, cel-miR-793, cel-miR-241-5p, and cel-miR-5549-5p could regulate the longevity-related pathways and inflammation signaling pathways, etc. Additionally, our data confirmed that SiNPs could disrupt the locomotion behavior and reduce the longevity by activating ins-7, daf-16, ftt-2, fat-5, and rho-1 genes in C. elegans.Conclusion: Our study showed that SiNPs induced the change of the whole transcriptome in C. elegans, and triggered negative effects on longevity, development, reproduction, and body morphogenesis. These data provide abundant clues to understand the molecular mechanisms of SiNPs in C. elegans.Keywords: SiNPs, genome microarrays, longevity, development, Caenorhabditis elegansLiang SDuan JHu HZhang JGao SJing HLi GSun ZDove Medical Pressarticlesinpsgenome microarrayslongevitydevelopmentc. elegansMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 5227-5237 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	sinps genome microarrays longevity development c. elegans Medicine (General) R5-920
spellingShingle	sinps genome microarrays longevity development c. elegans Medicine (General) R5-920 Liang S Duan J Hu H Zhang J Gao S Jing H Li G Sun Z Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
description	Shuang Liang,1,2,* Junchao Duan,1,2,* Hejing Hu,1,2 Jingyi Zhang,1,2 Shan Gao,2,3 Haiming Jing,2,3 Guojun Li,2,3 Zhiwei Sun1,2 1Department of Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing 100069, People’s Republic of China; 2Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, People’s Republic of China; 3Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhiwei SunSchool of Public Health, Capital Medical University, Beijing 100069, People’s Republic of ChinaEmail zwsun@ccmu.edu.cnGuojun LiBeijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, Beijing 100013, People’s Republic of ChinaEmail ligj@bjcdc.orgBackground: Large-scale production and application of amorphous silica nanoparticles (SiNPs) have enhanced the risk of human exposure to SiNPs. However, the toxic effects and the underlying biological mechanisms of SiNPs on Caenorhabditis elegans remain largely unclear.Purpose: This study was to investigate the genome-wide transcriptional alteration of SiNPs on C. elegans.Methods and Results: In this study, a total number of 3105 differentially expressed genes were identified in C. elegans. Among them, 1398 genes were significantly upregulated and 1707 genes were notably downregulated in C. elegans. Gene ontology analysis revealed that the significant change of gene functional categories triggered by SiNPs was focused on locomotion, determination of adult lifespan, reproduction, body morphogenesis, multicellular organism development, endoplasmic reticulum unfolded protein response, oocyte development, and nematode larval development. Meanwhile, we explored the regulated effects between microRNA and genes or signaling pathways. Pathway enrichment analysis and miRNA-gene-pathway-network displayed that 23 differential expression microRNA including cel-miR-85-3p, cel-miR-793, cel-miR-241-5p, and cel-miR-5549-5p could regulate the longevity-related pathways and inflammation signaling pathways, etc. Additionally, our data confirmed that SiNPs could disrupt the locomotion behavior and reduce the longevity by activating ins-7, daf-16, ftt-2, fat-5, and rho-1 genes in C. elegans.Conclusion: Our study showed that SiNPs induced the change of the whole transcriptome in C. elegans, and triggered negative effects on longevity, development, reproduction, and body morphogenesis. These data provide abundant clues to understand the molecular mechanisms of SiNPs in C. elegans.Keywords: SiNPs, genome microarrays, longevity, development, Caenorhabditis elegans
format	article
author	Liang S Duan J Hu H Zhang J Gao S Jing H Li G Sun Z
author_facet	Liang S Duan J Hu H Zhang J Gao S Jing H Li G Sun Z
author_sort	Liang S
title	Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
title_short	Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
title_full	Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
title_fullStr	Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
title_full_unstemmed	Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans
title_sort	comprehensive analysis of sinps on the genome-wide transcriptional changes in caenorhabditis elegans
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/88e8d3de30da41cabd92d5b3172f57f3
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Comprehensive Analysis of SiNPs on the Genome-Wide Transcriptional Changes in Caenorhabditis elegans

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