Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>

Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>

<i>Alternaria alternata</i> is the main pathogenic species of various crops, including kiwifruit (<i>Actinidia cinensis</i>). In this study, an antagonistic fungus, J-1, with high antifungal activity against <i>A. alternata</i> was isolated from <i>A. cinens...

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Autores principales: Wenzhi Li, Youhua Long, Feixu Mo, Ran Shu, Xianhui Yin, Xiaomao Wu, Rongquan Zhang, Zhuzhu Zhang, Linan He, Tingting Chen, Jia Chen
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
antagonistic fungus
<i>Fusicolla violacea</i>
<i>Alternaria alternata</i>
kiwifruit
biocontrol
Biology (General)
QH301-705.5
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spelling oai:doaj.org-article:88eb313f74554cb6a490d8fec76851602021-11-25T18:05:57ZAntifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>10.3390/jof71109372309-608Xhttps://doaj.org/article/88eb313f74554cb6a490d8fec76851602021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/937https://doaj.org/toc/2309-608X<i>Alternaria alternata</i> is the main pathogenic species of various crops, including kiwifruit (<i>Actinidia cinensis</i>). In this study, an antagonistic fungus, J-1, with high antifungal activity against <i>A. alternata</i> was isolated from <i>A. cinensis</i> “Hongyang.” The strain J-1 was identified as <i>Fusicolla violacea</i> via morphological identification and DNA sequencing. This study aimed to evaluate the antifungal activity and potential mechanism of the strain J-1 against <i>A. alternata</i>. The strain J-1 exhibited antifungal activity against <i>A. alternata</i>, with an inhibition rate of 66.1% in vitro. Aseptic filtrate (AF) produced by the strain J-1 could suppress the mycelial growth and conidia germination of <i>A. alternata</i> at the inhibition rates of 66.8% and 80%, respectively, as well as suppress the spread of <i>Alternaria</i> rot in fresh kiwifruit. We observed that many clusters of spherical protrusions appeared at the mycelial tips of <i>A. alternata</i> after treatment with 200 mL L<sup>−1</sup> AF of J-1. Scanning electron microscopy analysis results showed that the mycelial structures were bent and/or malformed and the surfaces were rough and protuberant. Variations in temperature, pH, and storage time had little effect on the antifungal activity of the AF. Moreover, the AF could damage the integrity of cell membranes and cause intracellular content leakage. Meanwhile, the chitinase and β-1,3-glucanase enzyme activities increased significantly, indicating that the function of <i>A. alternata</i> cell wall was seriously injured. Eleven antimicrobial metabolites were identified by gas chromatography–mass spectrometry (GC–MS). The strain J-I and its AF exhibited well broad-spectrum antifungal activity against <i>Diaporthe eres</i>, <i>Epicoccum sorghinum</i>, <i>Fusarium graminearum</i>, <i>Phomopsis</i> sp., and <i>Botryosphaeria dothidea</i>, with inhibition rates ranging from 34.4% to 75.1% and 42.7% to 75.2%, respectively. <i>Fusicolla violacea</i> J-1 is a potential biocontrol agent against <i>A. alternata</i> and other fungal phytopathogens.Wenzhi LiYouhua LongFeixu MoRan ShuXianhui YinXiaomao WuRongquan ZhangZhuzhu ZhangLinan HeTingting ChenJia ChenMDPI AGarticleantagonistic fungus<i>Fusicolla violacea</i><i>Alternaria alternata</i>kiwifruitbiocontrolBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 937, p 937 (2021)
institution DOAJ
collection DOAJ
language EN
topic antagonistic fungus
<i>Fusicolla violacea</i>
<i>Alternaria alternata</i>
kiwifruit
biocontrol
Biology (General)
QH301-705.5
spellingShingle antagonistic fungus
<i>Fusicolla violacea</i>
<i>Alternaria alternata</i>
kiwifruit
biocontrol
Biology (General)
QH301-705.5
Wenzhi Li
Youhua Long
Feixu Mo
Ran Shu
Xianhui Yin
Xiaomao Wu
Rongquan Zhang
Zhuzhu Zhang
Linan He
Tingting Chen
Jia Chen
Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
description <i>Alternaria alternata</i> is the main pathogenic species of various crops, including kiwifruit (<i>Actinidia cinensis</i>). In this study, an antagonistic fungus, J-1, with high antifungal activity against <i>A. alternata</i> was isolated from <i>A. cinensis</i> “Hongyang.” The strain J-1 was identified as <i>Fusicolla violacea</i> via morphological identification and DNA sequencing. This study aimed to evaluate the antifungal activity and potential mechanism of the strain J-1 against <i>A. alternata</i>. The strain J-1 exhibited antifungal activity against <i>A. alternata</i>, with an inhibition rate of 66.1% in vitro. Aseptic filtrate (AF) produced by the strain J-1 could suppress the mycelial growth and conidia germination of <i>A. alternata</i> at the inhibition rates of 66.8% and 80%, respectively, as well as suppress the spread of <i>Alternaria</i> rot in fresh kiwifruit. We observed that many clusters of spherical protrusions appeared at the mycelial tips of <i>A. alternata</i> after treatment with 200 mL L<sup>−1</sup> AF of J-1. Scanning electron microscopy analysis results showed that the mycelial structures were bent and/or malformed and the surfaces were rough and protuberant. Variations in temperature, pH, and storage time had little effect on the antifungal activity of the AF. Moreover, the AF could damage the integrity of cell membranes and cause intracellular content leakage. Meanwhile, the chitinase and β-1,3-glucanase enzyme activities increased significantly, indicating that the function of <i>A. alternata</i> cell wall was seriously injured. Eleven antimicrobial metabolites were identified by gas chromatography–mass spectrometry (GC–MS). The strain J-I and its AF exhibited well broad-spectrum antifungal activity against <i>Diaporthe eres</i>, <i>Epicoccum sorghinum</i>, <i>Fusarium graminearum</i>, <i>Phomopsis</i> sp., and <i>Botryosphaeria dothidea</i>, with inhibition rates ranging from 34.4% to 75.1% and 42.7% to 75.2%, respectively. <i>Fusicolla violacea</i> J-1 is a potential biocontrol agent against <i>A. alternata</i> and other fungal phytopathogens.
format article
author Wenzhi Li
Youhua Long
Feixu Mo
Ran Shu
Xianhui Yin
Xiaomao Wu
Rongquan Zhang
Zhuzhu Zhang
Linan He
Tingting Chen
Jia Chen
author_facet Wenzhi Li
Youhua Long
Feixu Mo
Ran Shu
Xianhui Yin
Xiaomao Wu
Rongquan Zhang
Zhuzhu Zhang
Linan He
Tingting Chen
Jia Chen
author_sort Wenzhi Li
title Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
title_short Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
title_full Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
title_fullStr Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
title_full_unstemmed Antifungal Activity and Biocontrol Mechanism of <i>Fusicolla violacea</i> J-1 against Soft Rot in Kiwifruit Caused by <i>Alternaria alternata</i>
title_sort antifungal activity and biocontrol mechanism of <i>fusicolla violacea</i> j-1 against soft rot in kiwifruit caused by <i>alternaria alternata</i>
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/88eb313f74554cb6a490d8fec7685160
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