Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. H...

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Autores principales:	Gabina Calderón-Rosete, Juan Antonio González-Barrios, Celia Piña-Leyva, Hayde Nallely Moreno-Sandoval, Manuel Lara-Lozano, Leonardo Rodríguez-Sosa
Formato:	article
Lenguaje:	EN
Publicado:	Pensoft Publishers 2021
Materias:	Zoology QL1-991
Acceso en línea:	https://doaj.org/article/88ef2d969ed9487993356fa369f1b164
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spelling	oai:doaj.org-article:88ef2d969ed9487993356fa369f1b1642021-11-21T04:30:18ZTranscriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii10.3897/zookeys.1072.730751313-2970https://doaj.org/article/88ef2d969ed9487993356fa369f1b1642021-11-01T00:00:00Zhttps://zookeys.pensoft.net/article/73075/download/pdf/https://zookeys.pensoft.net/article/73075/download/xml/https://zookeys.pensoft.net/article/73075/https://doaj.org/toc/1313-2970Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarus clarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94–100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P. clarkii use biosynthesis pathways and phototransduction cascades commons.Gabina Calderón-RoseteJuan Antonio González-BarriosCelia Piña-LeyvaHayde Nallely Moreno-SandovalManuel Lara-LozanoLeonardo Rodríguez-SosaPensoft PublishersarticleZoologyQL1-991ENZooKeys, Vol 1072, Iss , Pp 107-127 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zoology QL1-991
spellingShingle	Zoology QL1-991 Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
description	Crayfish serve as a model for studying the effect of environmental lighting on locomotor activity and neuroendocrine functions. The effects of light on this organism are mediated differentially by retinal and extraretinal photoreceptors located in the cerebroid ganglion and the pleonal nerve cord. However, some molecular aspects of the phototransduction cascade in the pleonal extraretinal photoreceptors remain unknown. In this study, transcriptome data from the pleonal nerve cord of the crayfish Procambarus clarkii (Girard,1852) were analyzed to identify transcripts that potentially interact with phototransduction process. The Illumina MiSeq System and the pipeline Phylogenetically Informed Annotation (PIA) were employed, which places uncharacterized genes into pre-calculated phylogenies of gene families. Here, for the first time 62 transcripts identified from the pleonal nerve cord that are related to light-interacting pathways are reported; they can be classified into the following 11 sets: 1) retinoid pathway in vertebrates and invertebrates, 2) photoreceptor specification, 3) rhabdomeric phototransduction, 4) opsins 5) ciliary phototransduction, 6) melanin synthesis, 7) pterin synthesis, 8) ommochrome synthesis, 9) heme synthesis, 10) diurnal clock, and 11) crystallins. Moreover, this analysis comparing the sequences located on the pleonal nerve cord to eyestalk sequences reported in other studies reveals 94–100% similarity between the 55 common proteins identified. These results show that both retinal and pleonal non-visual photoreceptors in the crayfish equally expressed the transcripts involved in light detection. Moreover, they suggest that the genes related to ocular and extraocular light perception in the crayfish P. clarkii use biosynthesis pathways and phototransduction cascades commons.
format	article
author	Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa
author_facet	Gabina Calderón-Rosete Juan Antonio González-Barrios Celia Piña-Leyva Hayde Nallely Moreno-Sandoval Manuel Lara-Lozano Leonardo Rodríguez-Sosa
author_sort	Gabina Calderón-Rosete
title	Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
title_short	Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
title_full	Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
title_fullStr	Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
title_full_unstemmed	Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii
title_sort	transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish procambarus clarkii
publisher	Pensoft Publishers
publishDate	2021
url	https://doaj.org/article/88ef2d969ed9487993356fa369f1b164
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Transcriptional identification of genes light-interacting in the extraretinal photoreceptors of the crayfish Procambarus clarkii

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