Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells.
Intestinal microfold (M) cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathw...
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2013
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oai:doaj.org-article:88f2443e33b84649b846b4d2a4e288212021-11-18T05:37:50ZDectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells.1544-91731545-788510.1371/journal.pbio.1001658https://doaj.org/article/88f2443e33b84649b846b4d2a4e288212013-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24068891/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Intestinal microfold (M) cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathway for delivery of secretory IgA (SIgA) to the gut-associated lymphoid tissue. However, although the consequences of SIgA uptake by M cells are now well known and described, the mechanisms whereby SIgA is selectively bound and taken up remain poorly understood. Here we first demonstrate that both the Cα1 region and glycosylation, more particularly sialic acid residues, are involved in M cell-mediated reverse transcytosis. Second, we found that SIgA is taken up by M cells via the Dectin-1 receptor, with the possible involvement of Siglec-5 acting as a co-receptor. Third, we establish that transcytosed SIgA is taken up by mucosal CX3CR1⁺ dendritic cells (DCs) via the DC-SIGN receptor. Fourth, we show that mucosal and systemic antibody responses against the HIV p24-SIgA complexes administered orally is strictly dependent on the expression of Dectin-1. Having deciphered the mechanisms leading to specific targeting of SIgA-based Ag complexes paves the way to the use of such a vehicle for mucosal vaccination against various infectious diseases.Nicolas RochereauDaniel DrocourtEric PerouzelVincent PavotPierre RedelinghuysGordon D BrownGerard TirabyXavier RoblinBernard VerrierChristian GeninBlaise CorthésyStéphane PaulPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 9, p e1001658 (2013) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Biology (General) QH301-705.5 |
| spellingShingle |
Biology (General) QH301-705.5 Nicolas Rochereau Daniel Drocourt Eric Perouzel Vincent Pavot Pierre Redelinghuys Gordon D Brown Gerard Tiraby Xavier Roblin Bernard Verrier Christian Genin Blaise Corthésy Stéphane Paul Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| description |
Intestinal microfold (M) cells possess a high transcytosis capacity and are able to transport a broad range of materials including particulate antigens, soluble macromolecules, and pathogens from the intestinal lumen to inductive sites of the mucosal immune system. M cells are also the primary pathway for delivery of secretory IgA (SIgA) to the gut-associated lymphoid tissue. However, although the consequences of SIgA uptake by M cells are now well known and described, the mechanisms whereby SIgA is selectively bound and taken up remain poorly understood. Here we first demonstrate that both the Cα1 region and glycosylation, more particularly sialic acid residues, are involved in M cell-mediated reverse transcytosis. Second, we found that SIgA is taken up by M cells via the Dectin-1 receptor, with the possible involvement of Siglec-5 acting as a co-receptor. Third, we establish that transcytosed SIgA is taken up by mucosal CX3CR1⁺ dendritic cells (DCs) via the DC-SIGN receptor. Fourth, we show that mucosal and systemic antibody responses against the HIV p24-SIgA complexes administered orally is strictly dependent on the expression of Dectin-1. Having deciphered the mechanisms leading to specific targeting of SIgA-based Ag complexes paves the way to the use of such a vehicle for mucosal vaccination against various infectious diseases. |
| format |
article |
| author |
Nicolas Rochereau Daniel Drocourt Eric Perouzel Vincent Pavot Pierre Redelinghuys Gordon D Brown Gerard Tiraby Xavier Roblin Bernard Verrier Christian Genin Blaise Corthésy Stéphane Paul |
| author_facet |
Nicolas Rochereau Daniel Drocourt Eric Perouzel Vincent Pavot Pierre Redelinghuys Gordon D Brown Gerard Tiraby Xavier Roblin Bernard Verrier Christian Genin Blaise Corthésy Stéphane Paul |
| author_sort |
Nicolas Rochereau |
| title |
Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| title_short |
Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| title_full |
Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| title_fullStr |
Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| title_full_unstemmed |
Dectin-1 is essential for reverse transcytosis of glycosylated SIgA-antigen complexes by intestinal M cells. |
| title_sort |
dectin-1 is essential for reverse transcytosis of glycosylated siga-antigen complexes by intestinal m cells. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/88f2443e33b84649b846b4d2a4e28821 |
| work_keys_str_mv |
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| _version_ |
1718424861417668608 |