Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida

Abstract QseC, a histidine sensor kinase of the QseBC two-component system, acts as a global regulator of bacterial stress resistance, biofilm formation, and virulence. The function of QseC in some bacteria is well understood, but not in Pasteurella multocida. We found that deleting qseC in P. multo...

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Autores principales:	Yang Yang, Pei Hu, Lixu Gao, Xiang Yuan, Philip R. Hardwidge, Tian Li, Pan Li, Fang He, Yuanyi Peng, Nengzhang Li
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Quorum sensing gene Deletion Virulence Vaccine Cross-protection Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/88f55860a44741e7b75a2156bfa40179
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spelling	oai:doaj.org-article:88f55860a44741e7b75a2156bfa401792021-11-21T12:04:33ZDeleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida10.1186/s13567-021-01009-61297-9716https://doaj.org/article/88f55860a44741e7b75a2156bfa401792021-11-01T00:00:00Zhttps://doi.org/10.1186/s13567-021-01009-6https://doaj.org/toc/1297-9716Abstract QseC, a histidine sensor kinase of the QseBC two-component system, acts as a global regulator of bacterial stress resistance, biofilm formation, and virulence. The function of QseC in some bacteria is well understood, but not in Pasteurella multocida. We found that deleting qseC in P. multocida serotype A:L3 significantly down-regulated bacterial virulence. The mutant had significantly reduced capsule production but increased resistance to oxidative stress and osmotic pressure. Deleting qseC led to a significant increase in qseB expression. Transcriptome sequencing analysis showed that 1245 genes were regulated by qseC, primarily those genes involved in capsule and LPS biosynthesis and export, biofilm formation, and iron uptake/utilization, as well as several immuno-protection related genes including ompA, ptfA, plpB, vacJ, and sodA. In addition to presenting strong immune protection against P. multocida serotypes A:L1 and A:L3 infection, live ΔqseC also exhibited protection against P. multocida serotype B:L2 and serotype F:L3 infection in a mouse model. The results indicate that QseC regulates capsular production and virulence in P. multocida. Furthermore, the qseC mutant can be used as an attenuated vaccine against P. multocida strains of multiple serotypes.Yang YangPei HuLixu GaoXiang YuanPhilip R. HardwidgeTian LiPan LiFang HeYuanyi PengNengzhang LiBMCarticleQuorum sensing geneDeletionVirulenceVaccineCross-protectionVeterinary medicineSF600-1100ENVeterinary Research, Vol 52, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Quorum sensing gene Deletion Virulence Vaccine Cross-protection Veterinary medicine SF600-1100
spellingShingle	Quorum sensing gene Deletion Virulence Vaccine Cross-protection Veterinary medicine SF600-1100 Yang Yang Pei Hu Lixu Gao Xiang Yuan Philip R. Hardwidge Tian Li Pan Li Fang He Yuanyi Peng Nengzhang Li Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
description	Abstract QseC, a histidine sensor kinase of the QseBC two-component system, acts as a global regulator of bacterial stress resistance, biofilm formation, and virulence. The function of QseC in some bacteria is well understood, but not in Pasteurella multocida. We found that deleting qseC in P. multocida serotype A:L3 significantly down-regulated bacterial virulence. The mutant had significantly reduced capsule production but increased resistance to oxidative stress and osmotic pressure. Deleting qseC led to a significant increase in qseB expression. Transcriptome sequencing analysis showed that 1245 genes were regulated by qseC, primarily those genes involved in capsule and LPS biosynthesis and export, biofilm formation, and iron uptake/utilization, as well as several immuno-protection related genes including ompA, ptfA, plpB, vacJ, and sodA. In addition to presenting strong immune protection against P. multocida serotypes A:L1 and A:L3 infection, live ΔqseC also exhibited protection against P. multocida serotype B:L2 and serotype F:L3 infection in a mouse model. The results indicate that QseC regulates capsular production and virulence in P. multocida. Furthermore, the qseC mutant can be used as an attenuated vaccine against P. multocida strains of multiple serotypes.
format	article
author	Yang Yang Pei Hu Lixu Gao Xiang Yuan Philip R. Hardwidge Tian Li Pan Li Fang He Yuanyi Peng Nengzhang Li
author_facet	Yang Yang Pei Hu Lixu Gao Xiang Yuan Philip R. Hardwidge Tian Li Pan Li Fang He Yuanyi Peng Nengzhang Li
author_sort	Yang Yang
title	Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
title_short	Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
title_full	Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
title_fullStr	Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
title_full_unstemmed	Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida
title_sort	deleting qsec downregulates virulence and promotes cross-protection in pasteurella multocida
publisher	BMC
publishDate	2021
url	https://doaj.org/article/88f55860a44741e7b75a2156bfa40179
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Deleting qseC downregulates virulence and promotes cross-protection in Pasteurella multocida

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