The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing

Longwei Lv,1 Yunsong Liu,1 Ping Zhang,1 Xiangsong Bai,1 Xiaohan Ma,1 Yuejun Wang,1 Hongyi Li,2 Li Wang,3 Yongsheng Zhou1 1Department of Prosthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, National...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lv L, Liu Y, Zhang P, Bai X, Ma X, Wang Y, Li H, Wang L, Zhou Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	TiO2 nanotubes RNA sequencing osteogenic differentiation epigenetics histone modification non-coding RNA Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/88fb9ec18ce044faba5d3d87f4da540a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:88fb9ec18ce044faba5d3d87f4da540a
record_format	dspace
spelling	oai:doaj.org-article:88fb9ec18ce044faba5d3d87f4da540a2021-12-02T07:56:33ZThe epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing1178-2013https://doaj.org/article/88fb9ec18ce044faba5d3d87f4da540a2018-09-01T00:00:00Zhttps://www.dovepress.com/the-epigenetic-mechanisms-of-nanotopography-guided-osteogenic-differen-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Longwei Lv,1 Yunsong Liu,1 Ping Zhang,1 Xiangsong Bai,1 Xiaohan Ma,1 Yuejun Wang,1 Hongyi Li,2 Li Wang,3 Yongsheng Zhou1 1Department of Prosthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, National Clinical Research Center for Oral Disease, Beijing Key Laboratory of Digital Stomatology, Beijing, People’s Republic of China; 2The key Laboratory of Advanced Functional Materials, Ministry of Education of China, College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, China; 3State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, People’s Republic of China Background: Nanotopography directs stem cell fate; however, the underlying mechanisms, especially those at the epigenetic level, remain vague. The TiO2-nanotube array, a classical example of nanotopography, is a good model to investigate topography–cell interactions because of its good controllability and easy manufacturing process. Previously, we found that a TiO2-nanotube array with an optimal diameter promoted osteogenic differentiation of human adipose-tissue-derived stem cells (hASCs). Methods: We used RNA sequencing and bioinformatics to reveal the overall gene expression profile of hASCs on TiO2-nanotube arrays. Results: Bioinformatics analyses revealed that the epigenetic regulatory network plays an important role in TiO2-nanotube-guided osteogenic differentiation. Changes in cell adhesion and cytoskeletal reorganization are linked to epigenetic alterations, including upregulation of KDM4E and downregulation of histone deacetylases. Meanwhile, microRNAs, including miR-24-1-5p, miR-24–3 p, miR-154–3 p, miR-154–5 p, miR-433–5 p, miR-589–3 p, and miR-589–5 p were downregulated, whereas miR-186–5 p and miR-770–5 p were upregulated. Long non-coding RNAs, including LINC00941, LINC01279, and ZFAS1, were downregulated in this process. Conclusion: Using next-generation sequencing, we illustrated the overall picture of the regulatory mechanisms of TiO2 nanotubes, thus providing a basis for future clinical applications of nanotopography in the field of bone tissue engineering. Our results offer insights into material-based nanomedicine and epigenetic therapy. Keywords: TiO2 nanotubes, RNA sequencing, osteogenic differentiation, epigenetics, histone modification, non-coding RNALv LLiu YZhang PBai XMa XWang YLi HWang LZhou YDove Medical PressarticleTiO2 nanotubesRNA sequencingosteogenic differentiationepigeneticshistone modificationnon-coding RNAMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5605-5623 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	TiO2 nanotubes RNA sequencing osteogenic differentiation epigenetics histone modification non-coding RNA Medicine (General) R5-920
spellingShingle	TiO2 nanotubes RNA sequencing osteogenic differentiation epigenetics histone modification non-coding RNA Medicine (General) R5-920 Lv L Liu Y Zhang P Bai X Ma X Wang Y Li H Wang L Zhou Y The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
description	Longwei Lv,1 Yunsong Liu,1 Ping Zhang,1 Xiangsong Bai,1 Xiaohan Ma,1 Yuejun Wang,1 Hongyi Li,2 Li Wang,3 Yongsheng Zhou1 1Department of Prosthodontics, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, National Clinical Research Center for Oral Disease, Beijing Key Laboratory of Digital Stomatology, Beijing, People’s Republic of China; 2The key Laboratory of Advanced Functional Materials, Ministry of Education of China, College of Materials Science and Engineering, Beijing University of Technology, Beijing 100124, China; 3State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, People’s Republic of China Background: Nanotopography directs stem cell fate; however, the underlying mechanisms, especially those at the epigenetic level, remain vague. The TiO2-nanotube array, a classical example of nanotopography, is a good model to investigate topography–cell interactions because of its good controllability and easy manufacturing process. Previously, we found that a TiO2-nanotube array with an optimal diameter promoted osteogenic differentiation of human adipose-tissue-derived stem cells (hASCs). Methods: We used RNA sequencing and bioinformatics to reveal the overall gene expression profile of hASCs on TiO2-nanotube arrays. Results: Bioinformatics analyses revealed that the epigenetic regulatory network plays an important role in TiO2-nanotube-guided osteogenic differentiation. Changes in cell adhesion and cytoskeletal reorganization are linked to epigenetic alterations, including upregulation of KDM4E and downregulation of histone deacetylases. Meanwhile, microRNAs, including miR-24-1-5p, miR-24–3 p, miR-154–3 p, miR-154–5 p, miR-433–5 p, miR-589–3 p, and miR-589–5 p were downregulated, whereas miR-186–5 p and miR-770–5 p were upregulated. Long non-coding RNAs, including LINC00941, LINC01279, and ZFAS1, were downregulated in this process. Conclusion: Using next-generation sequencing, we illustrated the overall picture of the regulatory mechanisms of TiO2 nanotubes, thus providing a basis for future clinical applications of nanotopography in the field of bone tissue engineering. Our results offer insights into material-based nanomedicine and epigenetic therapy. Keywords: TiO2 nanotubes, RNA sequencing, osteogenic differentiation, epigenetics, histone modification, non-coding RNA
format	article
author	Lv L Liu Y Zhang P Bai X Ma X Wang Y Li H Wang L Zhou Y
author_facet	Lv L Liu Y Zhang P Bai X Ma X Wang Y Li H Wang L Zhou Y
author_sort	Lv L
title	The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
title_short	The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
title_full	The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
title_fullStr	The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
title_full_unstemmed	The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
title_sort	epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/88fb9ec18ce044faba5d3d87f4da540a
work_keys_str_mv	AT lvl theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT liuy theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT zhangp theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT baix theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT max theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT wangy theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT lih theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT wangl theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT zhouy theepigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT lvl epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT liuy epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT zhangp epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT baix epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT max epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT wangy epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT lih epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT wangl epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing AT zhouy epigeneticmechanismsofnanotopographyguidedosteogenicdifferentiationofmesenchymalstemcellsviahighthroughputtranscriptomesequencing
_version_	1718399113003794432

The epigenetic mechanisms of nanotopography-guided osteogenic differentiation of mesenchymal stem cells via high-throughput transcriptome sequencing

Ejemplares similares