Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns

Modeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D...

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Autores principales:	Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Biotechnology TP248.13-248.65 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/88fd895a1fbb40f4a1f1b233ee036835
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spelling	oai:doaj.org-article:88fd895a1fbb40f4a1f1b233ee0368352021-12-02T16:19:39ZThree-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns10.1038/s41526-017-0011-22373-8065https://doaj.org/article/88fd895a1fbb40f4a1f1b233ee0368352017-02-01T00:00:00Zhttps://doi.org/10.1038/s41526-017-0011-2https://doaj.org/toc/2373-8065Modeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D) intestinal co-culture model containing multiple differentiated epithelial cell types and phagocytic macrophages with antibacterial function to study infection by multiple pathovars of Salmonella. This study is the first to show that these pathovars (known to possess different host adaptations, antibiotic resistance profiles and disease phenotypes), display markedly different colonization and intracellular co-localization patterns using this physiologically relevant new 3-D intestinal co-culture model. This advanced model, that integrates a key immune cell type important for Salmonella infection, offers a powerful new tool in understanding enteric pathogenesis and may lead to unexpected pathogenesis mechanisms and therapeutic targets that have been previously unobserved or unappreciated using other intestinal cell culture models.Jennifer BarrilaJiseon YangAurélie CrabbéShameema F. SarkerYulong LiuC. Mark OttMayra A. Nelman-GonzalezSimon J. ClemettSeth D. NydamRebecca J. ForsythRichard R. DavisBrian E. CrucianHeather QuiriarteKenneth L. RolandKaren BrennemanClarence SamsChristine LoscherCheryl A. NickersonNature PortfolioarticleBiotechnologyTP248.13-248.65PhysiologyQP1-981ENnpj Microgravity, Vol 3, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnology TP248.13-248.65 Physiology QP1-981
spellingShingle	Biotechnology TP248.13-248.65 Physiology QP1-981 Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
description	Modeling intestinal infection with NASA biotechnology: A new 3-D intestinal co-culture model with macrophages to study enteric infection Using spaceflight analog bioreactor technology, Cheryl Nickerson at Arizona State University and collaborators developed and validated a new three-dimensional (3-D) intestinal co-culture model containing multiple differentiated epithelial cell types and phagocytic macrophages with antibacterial function to study infection by multiple pathovars of Salmonella. This study is the first to show that these pathovars (known to possess different host adaptations, antibiotic resistance profiles and disease phenotypes), display markedly different colonization and intracellular co-localization patterns using this physiologically relevant new 3-D intestinal co-culture model. This advanced model, that integrates a key immune cell type important for Salmonella infection, offers a powerful new tool in understanding enteric pathogenesis and may lead to unexpected pathogenesis mechanisms and therapeutic targets that have been previously unobserved or unappreciated using other intestinal cell culture models.
format	article
author	Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson
author_facet	Jennifer Barrila Jiseon Yang Aurélie Crabbé Shameema F. Sarker Yulong Liu C. Mark Ott Mayra A. Nelman-Gonzalez Simon J. Clemett Seth D. Nydam Rebecca J. Forsyth Richard R. Davis Brian E. Crucian Heather Quiriarte Kenneth L. Roland Karen Brenneman Clarence Sams Christine Loscher Cheryl A. Nickerson
author_sort	Jennifer Barrila
title	Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
title_short	Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
title_full	Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
title_fullStr	Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
title_full_unstemmed	Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns
title_sort	three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study salmonella enterica colonization patterns
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/88fd895a1fbb40f4a1f1b233ee036835
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Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns

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