Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results

Background: The screening ofTrypanosoma cruzi-infected blood donors using two serological techniques frequently leads to conflicting results. This fact prompted us to evaluate the diagnostic performance of four “in-house” immunodiagnostic tests and two commercially available enzyme-linked immunosorb...

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Autores principales:	Márcia M Ferreira-Silva, Gilberto de Araújo Pereira, Virmondes Rodrigues-Júnior, Wendell SF Meira, Fernando V Basques, Dante Mário Langhi-Júnior, Marcelo Romanelli, Eufrosina S Umezawa, Norival Késper-Júnior, Francisco Louzada-Neto, José O. Bordin, Helio Moraes-Souza
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Trypanosoma cruzi Chagas disease Immunodiagnosis Blood donors Inconclusive results TESA-blot Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/890295d9f59c4a53aa6599c5f2000e52
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spelling	oai:doaj.org-article:890295d9f59c4a53aa6599c5f2000e522021-11-04T04:36:58ZChagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results2531-137910.1016/j.htct.2020.06.016https://doaj.org/article/890295d9f59c4a53aa6599c5f2000e522021-10-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2531137920301188https://doaj.org/toc/2531-1379Background: The screening ofTrypanosoma cruzi-infected blood donors using two serological techniques frequently leads to conflicting results. This fact prompted us to evaluate the diagnostic performance of four “in-house” immunodiagnostic tests and two commercially available enzyme-linked immunosorbent assays (ELISAs). Material and Methods: One hundred and seventy-nine blood donors, whose screening for Chagas disease was doubtful, underwent three in-house ELISAs, one in-house immunoblotting test (TESA-blot), and two commercial ELISAs (bioMérieux and Wiener) in an attempt to define the presence or absence of infection. Simultaneously, 29 donors with previous positive results from three conventional serological tests and 30 donors with constant negative results were evaluated. Results: The ELISA-Wiener showed the highest rate in sensitivity (98.92%) and the ELISA-bioMérieux, the highest specificity (99.45%), followed by the TESA-blot, which showed superior performance, with lower false-negative (2.18%) and false-positive (1.12%) rates. In series, the combination composed of the TESA-blot and ELISA-bioMérieux showed slightly superior performance, with trifunctional protein deficiency (TFP) = 0.01%. Conclusion: Our study confirms the high sensitivity and specificity of commercial kits. To confirm the presence or absence of T. cruzi infection, the combination of TESA-blot and ELISA-bioMérieux may be suggested as the best alternative. Individually, the TESA-blot performed the closest to the gold standard; however, it is not commercially available.Márcia M Ferreira-SilvaGilberto de Araújo PereiraVirmondes Rodrigues-JúniorWendell SF MeiraFernando V BasquesDante Mário Langhi-JúniorMarcelo RomanelliEufrosina S UmezawaNorival Késper-JúniorFrancisco Louzada-NetoJosé O. BordinHelio Moraes-SouzaElsevierarticleTrypanosoma cruziChagas diseaseImmunodiagnosisBlood donorsInconclusive resultsTESA-blotDiseases of the blood and blood-forming organsRC633-647.5ENHematology, Transfusion and Cell Therapy, Vol 43, Iss 4, Pp 410-416 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Trypanosoma cruzi Chagas disease Immunodiagnosis Blood donors Inconclusive results TESA-blot Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	Trypanosoma cruzi Chagas disease Immunodiagnosis Blood donors Inconclusive results TESA-blot Diseases of the blood and blood-forming organs RC633-647.5 Márcia M Ferreira-Silva Gilberto de Araújo Pereira Virmondes Rodrigues-Júnior Wendell SF Meira Fernando V Basques Dante Mário Langhi-Júnior Marcelo Romanelli Eufrosina S Umezawa Norival Késper-Júnior Francisco Louzada-Neto José O. Bordin Helio Moraes-Souza Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
description	Background: The screening ofTrypanosoma cruzi-infected blood donors using two serological techniques frequently leads to conflicting results. This fact prompted us to evaluate the diagnostic performance of four “in-house” immunodiagnostic tests and two commercially available enzyme-linked immunosorbent assays (ELISAs). Material and Methods: One hundred and seventy-nine blood donors, whose screening for Chagas disease was doubtful, underwent three in-house ELISAs, one in-house immunoblotting test (TESA-blot), and two commercial ELISAs (bioMérieux and Wiener) in an attempt to define the presence or absence of infection. Simultaneously, 29 donors with previous positive results from three conventional serological tests and 30 donors with constant negative results were evaluated. Results: The ELISA-Wiener showed the highest rate in sensitivity (98.92%) and the ELISA-bioMérieux, the highest specificity (99.45%), followed by the TESA-blot, which showed superior performance, with lower false-negative (2.18%) and false-positive (1.12%) rates. In series, the combination composed of the TESA-blot and ELISA-bioMérieux showed slightly superior performance, with trifunctional protein deficiency (TFP) = 0.01%. Conclusion: Our study confirms the high sensitivity and specificity of commercial kits. To confirm the presence or absence of T. cruzi infection, the combination of TESA-blot and ELISA-bioMérieux may be suggested as the best alternative. Individually, the TESA-blot performed the closest to the gold standard; however, it is not commercially available.
format	article
author	Márcia M Ferreira-Silva Gilberto de Araújo Pereira Virmondes Rodrigues-Júnior Wendell SF Meira Fernando V Basques Dante Mário Langhi-Júnior Marcelo Romanelli Eufrosina S Umezawa Norival Késper-Júnior Francisco Louzada-Neto José O. Bordin Helio Moraes-Souza
author_facet	Márcia M Ferreira-Silva Gilberto de Araújo Pereira Virmondes Rodrigues-Júnior Wendell SF Meira Fernando V Basques Dante Mário Langhi-Júnior Marcelo Romanelli Eufrosina S Umezawa Norival Késper-Júnior Francisco Louzada-Neto José O. Bordin Helio Moraes-Souza
author_sort	Márcia M Ferreira-Silva
title	Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
title_short	Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
title_full	Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
title_fullStr	Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
title_full_unstemmed	Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results
title_sort	chagas disease: performance analysis of immunodiagnostic tests anti-trypanosoma cruzi in blood donors with inconclusive screening results
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/890295d9f59c4a53aa6599c5f2000e52
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Chagas disease: Performance analysis of immunodiagnostic tests anti-Trypanosoma cruzi in blood donors with inconclusive screening results

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