Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence

Abstract The capsular polysaccharide (CPS) of Streptococcus suis defines various serotypes based on its composition and structure. Though serotype switching has been suggested to occur between S. suis strains, its impact on pathogenicity and virulence remains unknown. Herein, we experimentally gener...

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Autores principales:	Masatoshi Okura, Jean-Philippe Auger, Tomoyuki Shibahara, Guillaume Goyette-Desjardins, Marie-Rose Van Calsteren, Fumito Maruyama, Mikihiko Kawai, Makoto Osaki, Mariela Segura, Marcelo Gottschalk, Daisuke Takamatsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8909718f37b5419f856f233e9867f9ea
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spelling	oai:doaj.org-article:8909718f37b5419f856f233e9867f9ea2021-12-02T16:35:55ZCapsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence10.1038/s41598-021-85882-32045-2322https://doaj.org/article/8909718f37b5419f856f233e9867f9ea2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85882-3https://doaj.org/toc/2045-2322Abstract The capsular polysaccharide (CPS) of Streptococcus suis defines various serotypes based on its composition and structure. Though serotype switching has been suggested to occur between S. suis strains, its impact on pathogenicity and virulence remains unknown. Herein, we experimentally generated S. suis serotype-switched mutants from a serotype 2 strain that express the serotype 3, 4, 7, 8, 9, or 14 CPS. The effects of serotype switching were then investigated with regards to classical properties conferred by presence of the serotype 2 CPS, including adhesion to/invasion of epithelial cells, resistance to phagocytosis by macrophages, killing by whole blood, dendritic cell-derived pro-inflammatory mediator production and virulence using mouse and porcine infection models. Results demonstrated that these properties on host cell interactions were differentially modulated depending on the switched serotypes, although some different mutations other than loci of CPS-related genes were found in each the serotype-switched mutant. Among the serotype-switched mutants, the mutant expressing the serotype 8 CPS was hyper-virulent, whereas mutants expressing the serotype 3 or 4 CPSs had reduced virulence. By contrast, switching to serotype 7, 9, or 14 CPSs had little to no effect. These findings suggest that serotype switching can drastically alter S. suis virulence and host cell interactions.Masatoshi OkuraJean-Philippe AugerTomoyuki ShibaharaGuillaume Goyette-DesjardinsMarie-Rose Van CalsterenFumito MaruyamaMikihiko KawaiMakoto OsakiMariela SeguraMarcelo GottschalkDaisuke TakamatsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masatoshi Okura Jean-Philippe Auger Tomoyuki Shibahara Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Fumito Maruyama Mikihiko Kawai Makoto Osaki Mariela Segura Marcelo Gottschalk Daisuke Takamatsu Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
description	Abstract The capsular polysaccharide (CPS) of Streptococcus suis defines various serotypes based on its composition and structure. Though serotype switching has been suggested to occur between S. suis strains, its impact on pathogenicity and virulence remains unknown. Herein, we experimentally generated S. suis serotype-switched mutants from a serotype 2 strain that express the serotype 3, 4, 7, 8, 9, or 14 CPS. The effects of serotype switching were then investigated with regards to classical properties conferred by presence of the serotype 2 CPS, including adhesion to/invasion of epithelial cells, resistance to phagocytosis by macrophages, killing by whole blood, dendritic cell-derived pro-inflammatory mediator production and virulence using mouse and porcine infection models. Results demonstrated that these properties on host cell interactions were differentially modulated depending on the switched serotypes, although some different mutations other than loci of CPS-related genes were found in each the serotype-switched mutant. Among the serotype-switched mutants, the mutant expressing the serotype 8 CPS was hyper-virulent, whereas mutants expressing the serotype 3 or 4 CPSs had reduced virulence. By contrast, switching to serotype 7, 9, or 14 CPSs had little to no effect. These findings suggest that serotype switching can drastically alter S. suis virulence and host cell interactions.
format	article
author	Masatoshi Okura Jean-Philippe Auger Tomoyuki Shibahara Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Fumito Maruyama Mikihiko Kawai Makoto Osaki Mariela Segura Marcelo Gottschalk Daisuke Takamatsu
author_facet	Masatoshi Okura Jean-Philippe Auger Tomoyuki Shibahara Guillaume Goyette-Desjardins Marie-Rose Van Calsteren Fumito Maruyama Mikihiko Kawai Makoto Osaki Mariela Segura Marcelo Gottschalk Daisuke Takamatsu
author_sort	Masatoshi Okura
title	Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
title_short	Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
title_full	Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
title_fullStr	Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
title_full_unstemmed	Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence
title_sort	capsular polysaccharide switching in streptococcus suis modulates host cell interactions and virulence
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8909718f37b5419f856f233e9867f9ea
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Capsular polysaccharide switching in Streptococcus suis modulates host cell interactions and virulence

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