A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening
Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells requires new tools such as tetramers for rapid cell s...
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Nature Portfolio
2021
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oai:doaj.org-article:890ce0d044f14d9ebfb2c8235af6b09b2021-12-02T15:09:06ZA novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening10.1038/s41598-021-96560-92045-2322https://doaj.org/article/890ce0d044f14d9ebfb2c8235af6b09b2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96560-9https://doaj.org/toc/2045-2322Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells requires new tools such as tetramers for rapid cell staining or sorting, as well as for the identification of new peptides capable to bind to the HLA-E pocket. To this aim, we have developed an optimal photosensitive peptide to generate stable HLA-E/pUV complexes allowing high-throughput production of new HLA-E/peptide complexes by peptide exchange. We characterized the UV exchange by ELISA and improved the peptide exchange readout using size exclusion chromatography. This novel approach for complex quantification is indeed very important to perform tetramerization of MHC/peptide complexes with the high quality required for detection of specific T cells. Our approach allows the rapid screening of peptides capable of binding to the non-classical human HLA-E allele, paving the way for the development of new therapeutic approaches based on the detection of HLA-E-restricted T cells.Juliette VaursGaël DouchinKlara EchasserieauRomain OgerNicolas JouandAgnès FortunLeslie HesnardMikaël CroyalFrédéric PecorariNadine GervoisKarine BernardeauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Juliette Vaurs Gaël Douchin Klara Echasserieau Romain Oger Nicolas Jouand Agnès Fortun Leslie Hesnard Mikaël Croyal Frédéric Pecorari Nadine Gervois Karine Bernardeau A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| description |
Abstract Over the past two decades, there has been a great interest in the study of HLA-E-restricted αβ T cells during bacterial and viral infections, including recently SARS-CoV-2 infection. Phenotyping of these specific HLA-E-restricted T cells requires new tools such as tetramers for rapid cell staining or sorting, as well as for the identification of new peptides capable to bind to the HLA-E pocket. To this aim, we have developed an optimal photosensitive peptide to generate stable HLA-E/pUV complexes allowing high-throughput production of new HLA-E/peptide complexes by peptide exchange. We characterized the UV exchange by ELISA and improved the peptide exchange readout using size exclusion chromatography. This novel approach for complex quantification is indeed very important to perform tetramerization of MHC/peptide complexes with the high quality required for detection of specific T cells. Our approach allows the rapid screening of peptides capable of binding to the non-classical human HLA-E allele, paving the way for the development of new therapeutic approaches based on the detection of HLA-E-restricted T cells. |
| format |
article |
| author |
Juliette Vaurs Gaël Douchin Klara Echasserieau Romain Oger Nicolas Jouand Agnès Fortun Leslie Hesnard Mikaël Croyal Frédéric Pecorari Nadine Gervois Karine Bernardeau |
| author_facet |
Juliette Vaurs Gaël Douchin Klara Echasserieau Romain Oger Nicolas Jouand Agnès Fortun Leslie Hesnard Mikaël Croyal Frédéric Pecorari Nadine Gervois Karine Bernardeau |
| author_sort |
Juliette Vaurs |
| title |
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| title_short |
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| title_full |
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| title_fullStr |
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| title_full_unstemmed |
A novel and efficient approach to high-throughput production of HLA-E/peptide monomer for T-cell epitope screening |
| title_sort |
novel and efficient approach to high-throughput production of hla-e/peptide monomer for t-cell epitope screening |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/890ce0d044f14d9ebfb2c8235af6b09b |
| work_keys_str_mv |
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