Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula

Background: Jianpi-Qushi-Heluo formula (JQHF) has been used to treat idiopathic membranous nephropathy (IMN) in hospitals for many years. Purpose: Elucidating the protective effect and exploring the potential mechanism of JQHF against IMN. Methods: Passive Heymann nephritis (PHN) was induced in rats...

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Autores principales:	Xin-Hui Wang, Ya-Nan Yang, Ying Liang, Rui Lang, Qin Zeng, Lei Yan, Ren-Huan Yu, Chong-Ming Wu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Idiopathic membranous nephropathy (IMN) Passive Heymann nephritis (PHN) Jianpi Qushi Heluo formula (JQHF) Gut microbiota Subdoligranulum variabile Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8913f1e8b09f4e7f9dff7382c4399366
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spelling	oai:doaj.org-article:8913f1e8b09f4e7f9dff7382c43993662021-12-02T04:59:08ZStructural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula0753-332210.1016/j.biopha.2021.112475https://doaj.org/article/8913f1e8b09f4e7f9dff7382c43993662022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221012610https://doaj.org/toc/0753-3322Background: Jianpi-Qushi-Heluo formula (JQHF) has been used to treat idiopathic membranous nephropathy (IMN) in hospitals for many years. Purpose: Elucidating the protective effect and exploring the potential mechanism of JQHF against IMN. Methods: Passive Heymann nephritis (PHN) was induced in rats by a single tail vein injection of anti-Fx1A antiserum. Then, the animals were treated with JQHF at 16.2 g/kg or 32.4 g/kg, with benzepril (10 mg/kg) as a positive control. Renal function was evaluated by biochemical measurements and pathological testing. Fecal samples were collected before and after treatment to analyze the gut microbiota composition by shotgun whole metagenome sequencing. Results: JQHF exhibited potent efficacy in ameliorating PHN at both doses, as revealed by decreasing the deposition of IgG and C5b-9, relieving podocyte injury, and reducing glomerular and tubular cell apoptosis. The lower dose was corresponding to the clinical dosage and showed better therapeutic effects than the higher dose. Metagenomic analysis showed that gavage with 16.2 g/kg of JQHF shifted the structure of the gut microbiota in PHN rats and significantly increased the relative abundances of Prevotella copri, Lactobacillus vaginalis and Subdoligranulum variabile. Particularly, S. variabile was strongly negatively correlated with serum levels of TC and TG, the deposition of IgG and C5b-9, and apoptosis of glomerular cells. Conclusions: The JQHF is an effective agent for the treatment of experimental PHN. The PHN-allevating effect of JQHF is associated with specific alternation of gut microbiota.Xin-Hui WangYa-Nan YangYing LiangRui LangQin ZengLei YanRen-Huan YuChong-Ming WuElsevierarticleIdiopathic membranous nephropathy (IMN)Passive Heymann nephritis (PHN)Jianpi Qushi Heluo formula (JQHF)Gut microbiotaSubdoligranulum variabileTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112475- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Idiopathic membranous nephropathy (IMN) Passive Heymann nephritis (PHN) Jianpi Qushi Heluo formula (JQHF) Gut microbiota Subdoligranulum variabile Therapeutics. Pharmacology RM1-950
spellingShingle	Idiopathic membranous nephropathy (IMN) Passive Heymann nephritis (PHN) Jianpi Qushi Heluo formula (JQHF) Gut microbiota Subdoligranulum variabile Therapeutics. Pharmacology RM1-950 Xin-Hui Wang Ya-Nan Yang Ying Liang Rui Lang Qin Zeng Lei Yan Ren-Huan Yu Chong-Ming Wu Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
description	Background: Jianpi-Qushi-Heluo formula (JQHF) has been used to treat idiopathic membranous nephropathy (IMN) in hospitals for many years. Purpose: Elucidating the protective effect and exploring the potential mechanism of JQHF against IMN. Methods: Passive Heymann nephritis (PHN) was induced in rats by a single tail vein injection of anti-Fx1A antiserum. Then, the animals were treated with JQHF at 16.2 g/kg or 32.4 g/kg, with benzepril (10 mg/kg) as a positive control. Renal function was evaluated by biochemical measurements and pathological testing. Fecal samples were collected before and after treatment to analyze the gut microbiota composition by shotgun whole metagenome sequencing. Results: JQHF exhibited potent efficacy in ameliorating PHN at both doses, as revealed by decreasing the deposition of IgG and C5b-9, relieving podocyte injury, and reducing glomerular and tubular cell apoptosis. The lower dose was corresponding to the clinical dosage and showed better therapeutic effects than the higher dose. Metagenomic analysis showed that gavage with 16.2 g/kg of JQHF shifted the structure of the gut microbiota in PHN rats and significantly increased the relative abundances of Prevotella copri, Lactobacillus vaginalis and Subdoligranulum variabile. Particularly, S. variabile was strongly negatively correlated with serum levels of TC and TG, the deposition of IgG and C5b-9, and apoptosis of glomerular cells. Conclusions: The JQHF is an effective agent for the treatment of experimental PHN. The PHN-allevating effect of JQHF is associated with specific alternation of gut microbiota.
format	article
author	Xin-Hui Wang Ya-Nan Yang Ying Liang Rui Lang Qin Zeng Lei Yan Ren-Huan Yu Chong-Ming Wu
author_facet	Xin-Hui Wang Ya-Nan Yang Ying Liang Rui Lang Qin Zeng Lei Yan Ren-Huan Yu Chong-Ming Wu
author_sort	Xin-Hui Wang
title	Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
title_short	Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
title_full	Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
title_fullStr	Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
title_full_unstemmed	Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula
title_sort	structural modulation of gut microbiota during alleviation of experimental passive heymann nephritis in rats by a traditional chinese herbal formula
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/8913f1e8b09f4e7f9dff7382c4399366
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_version_	1718400900775542784

Structural modulation of gut microbiota during alleviation of experimental passive Heymann nephritis in rats by a traditional Chinese herbal formula

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