miR-370-3p Regulates Adipogenesis through Targeting Mknk1

Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat ac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Peiwen Zhang, Xinrong Li, Shunhua Zhang, Shuang Wu, Qian Xiao, Yang Gu, Xinyu Guo, Xutao Lin, Lei Chen, Ye Zhao, Lili Niu, Guoqing Tang, Yanzhi Jiang, Linyuan Shen, Li Zhu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	miR-370-3p adipogenesis proliferation differentiation fatty acid composition Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/891a4fa04c42468a9c3e346097821501
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:891a4fa04c42468a9c3e346097821501
record_format	dspace
spelling	oai:doaj.org-article:891a4fa04c42468a9c3e3460978215012021-11-25T18:28:30ZmiR-370-3p Regulates Adipogenesis through Targeting Mknk110.3390/molecules262269261420-3049https://doaj.org/article/891a4fa04c42468a9c3e3460978215012021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6926https://doaj.org/toc/1420-3049Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1.Peiwen ZhangXinrong LiShunhua ZhangShuang WuQian XiaoYang GuXinyu GuoXutao LinLei ChenYe ZhaoLili NiuGuoqing TangYanzhi JiangLinyuan ShenLi ZhuMDPI AGarticlemiR-370-3padipogenesisproliferationdifferentiationfatty acid compositionOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6926, p 6926 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-370-3p adipogenesis proliferation differentiation fatty acid composition Organic chemistry QD241-441
spellingShingle	miR-370-3p adipogenesis proliferation differentiation fatty acid composition Organic chemistry QD241-441 Peiwen Zhang Xinrong Li Shunhua Zhang Shuang Wu Qian Xiao Yang Gu Xinyu Guo Xutao Lin Lei Chen Ye Zhao Lili Niu Guoqing Tang Yanzhi Jiang Linyuan Shen Li Zhu miR-370-3p Regulates Adipogenesis through Targeting Mknk1
description	Excessive fat accumulation can lead to obesity, diabetes, hyperlipidemia, atherosclerosis, and other diseases. MicroRNAs are a class of microRNAs that regulate gene expression and are highly conserved in function among species. microRNAs have been shown to act as regulatory factors to inhibit fat accumulation in the body. We found that miR-370-3p was expressed at lower levels in the fat mass of mice on a high-fat diet than in mice on a normal control diet. Furthermore, our data showed that the overexpression of miR-370-3p significantly suppressed the mRNA expression levels of adipogenic markers. Thus, miR-370-3p overexpression reduced lipid accumulation. Conversely, the inhibition of miR-370-3p suppressed 3T3-L1 preadipocyte proliferation and promoted preadipocyte differentiation. In addition, Mknk1, a target gene of miR-370-3p, plays an opposing role in preadipocyte proliferation and differentiation. Moreover, consistent results from in vitro as well as in vivo experiments suggest that the inhibition of fat accumulation by miR-370-3p may result from the inhibition of saturated fatty acids that promote the accumulation of polyunsaturated fatty acids. In conclusion, these results suggest that miR-370-3p plays an important role in adipogenesis and fatty acid metabolism through the regulation of Mknk1.
format	article
author	Peiwen Zhang Xinrong Li Shunhua Zhang Shuang Wu Qian Xiao Yang Gu Xinyu Guo Xutao Lin Lei Chen Ye Zhao Lili Niu Guoqing Tang Yanzhi Jiang Linyuan Shen Li Zhu
author_facet	Peiwen Zhang Xinrong Li Shunhua Zhang Shuang Wu Qian Xiao Yang Gu Xinyu Guo Xutao Lin Lei Chen Ye Zhao Lili Niu Guoqing Tang Yanzhi Jiang Linyuan Shen Li Zhu
author_sort	Peiwen Zhang
title	miR-370-3p Regulates Adipogenesis through Targeting Mknk1
title_short	miR-370-3p Regulates Adipogenesis through Targeting Mknk1
title_full	miR-370-3p Regulates Adipogenesis through Targeting Mknk1
title_fullStr	miR-370-3p Regulates Adipogenesis through Targeting Mknk1
title_full_unstemmed	miR-370-3p Regulates Adipogenesis through Targeting Mknk1
title_sort	mir-370-3p regulates adipogenesis through targeting mknk1
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/891a4fa04c42468a9c3e346097821501
work_keys_str_mv	AT peiwenzhang mir3703pregulatesadipogenesisthroughtargetingmknk1 AT xinrongli mir3703pregulatesadipogenesisthroughtargetingmknk1 AT shunhuazhang mir3703pregulatesadipogenesisthroughtargetingmknk1 AT shuangwu mir3703pregulatesadipogenesisthroughtargetingmknk1 AT qianxiao mir3703pregulatesadipogenesisthroughtargetingmknk1 AT yanggu mir3703pregulatesadipogenesisthroughtargetingmknk1 AT xinyuguo mir3703pregulatesadipogenesisthroughtargetingmknk1 AT xutaolin mir3703pregulatesadipogenesisthroughtargetingmknk1 AT leichen mir3703pregulatesadipogenesisthroughtargetingmknk1 AT yezhao mir3703pregulatesadipogenesisthroughtargetingmknk1 AT liliniu mir3703pregulatesadipogenesisthroughtargetingmknk1 AT guoqingtang mir3703pregulatesadipogenesisthroughtargetingmknk1 AT yanzhijiang mir3703pregulatesadipogenesisthroughtargetingmknk1 AT linyuanshen mir3703pregulatesadipogenesisthroughtargetingmknk1 AT lizhu mir3703pregulatesadipogenesisthroughtargetingmknk1
_version_	1718411088397074432

miR-370-3p Regulates Adipogenesis through Targeting Mknk1

Ejemplares similares