Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats

Introduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using...

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Autores principales:	Arief Adi Saputro, Taufiqurrachman Nasihun, Atina Hussaana
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2021
Materias:	propolis extract (cmce method) tnf-α levels crp levels Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/892e310d5500457dab8b2dbb51eda973
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spelling	oai:doaj.org-article:892e310d5500457dab8b2dbb51eda9732021-11-25T13:16:54ZPropolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats2085-15452339-093X10.30659/sainsmed.v12i1.5467https://doaj.org/article/892e310d5500457dab8b2dbb51eda9732021-04-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/5467https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using continuous multi-stage countercurrent Extraction (CMCE propolis extract) contains active balsamic, caffeic acid phenetyl ester (CAPE), and flavonoids capable of affecting pro-inflammatory cytokines. Objective: to investigate the effect of CMCE propolis extract on TNF-α and CRP levels in CCl4-induced liver damage. Methods: In this study using a post test only control group design, 28 male Wistar rats were divided into four groups. The control group (C-G) was given CCl4 0.4 ml/200 g on day 14. The P3-G, P7-G, P14-G groups were treated with 3.6 mg/200 g, 7.2 mg/200 g, 14.4 mg/200 g CMCE propolis extract for 14 days and given CCl4 0.4 ml/g on day 14. The TNF-α and CRP levels were assessed using the ELISA kit one-way ANOVA and Post Hoc test (LSD) were applied for the statistical analysis. Results: TNF-α level in P14-G group (3.26 ± 0.93), P7-G (6.17+0.78), and P3-G (10.4±1.34) were significantly lower than that of C-G (17.29+1.56) (p<0.05). CRP levels in P14-G group (0.61±0.09), P7-G (1.38+0.48), and P3-G (12.01±0.65) were significantly lower than those of C-G (2.95+0.668) (p<0.05). The greatest reduction in TNF-α & CRP level was found in the group given propolis at a dose of 14.4 mg/200 g. Conclusion: The administration of CMCE propolis extract reduces TNF-α & CRP levels in CCl4-induced liver damage.Arief Adi SaputroTaufiqurrachman NasihunAtina HussaanaUniversitas Sultan Agung Semarangarticlepropolis extract (cmce method)tnf-α levelscrp levelsMedicine (General)R5-920ENSains Medika, Vol 12, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis extract (cmce method) tnf-α levels crp levels Medicine (General) R5-920
spellingShingle	propolis extract (cmce method) tnf-α levels crp levels Medicine (General) R5-920 Arief Adi Saputro Taufiqurrachman Nasihun Atina Hussaana Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
description	Introduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-α), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using continuous multi-stage countercurrent Extraction (CMCE propolis extract) contains active balsamic, caffeic acid phenetyl ester (CAPE), and flavonoids capable of affecting pro-inflammatory cytokines. Objective: to investigate the effect of CMCE propolis extract on TNF-α and CRP levels in CCl4-induced liver damage. Methods: In this study using a post test only control group design, 28 male Wistar rats were divided into four groups. The control group (C-G) was given CCl4 0.4 ml/200 g on day 14. The P3-G, P7-G, P14-G groups were treated with 3.6 mg/200 g, 7.2 mg/200 g, 14.4 mg/200 g CMCE propolis extract for 14 days and given CCl4 0.4 ml/g on day 14. The TNF-α and CRP levels were assessed using the ELISA kit one-way ANOVA and Post Hoc test (LSD) were applied for the statistical analysis. Results: TNF-α level in P14-G group (3.26 ± 0.93), P7-G (6.17+0.78), and P3-G (10.4±1.34) were significantly lower than that of C-G (17.29+1.56) (p<0.05). CRP levels in P14-G group (0.61±0.09), P7-G (1.38+0.48), and P3-G (12.01±0.65) were significantly lower than those of C-G (2.95+0.668) (p<0.05). The greatest reduction in TNF-α & CRP level was found in the group given propolis at a dose of 14.4 mg/200 g. Conclusion: The administration of CMCE propolis extract reduces TNF-α & CRP levels in CCl4-induced liver damage.
format	article
author	Arief Adi Saputro Taufiqurrachman Nasihun Atina Hussaana
author_facet	Arief Adi Saputro Taufiqurrachman Nasihun Atina Hussaana
author_sort	Arief Adi Saputro
title	Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
title_short	Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
title_full	Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
title_fullStr	Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
title_full_unstemmed	Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats
title_sort	propolis extracted using cmce decreases tnf–α and c-reactive protein level of ccl4- induced liver damage in rats
publisher	Universitas Sultan Agung Semarang
publishDate	2021
url	https://doaj.org/article/892e310d5500457dab8b2dbb51eda973
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Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats

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